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The map label for this gene is yfkE [H]

Identifier: 221632245

GI number: 221632245

Start: 218177

End: 219505

Strand: Reverse

Name: yfkE [H]

Synonym: trd_0211

Alternate gene names: 221632245

Gene position: 219505-218177 (Counterclockwise)

Preceding gene: 221632246

Following gene: 221632244

Centisome position: 10.96

GC content: 65.16

Gene sequence:

>1329_bases
ATGCGCCGGTGGCTCGCGGGGCTGCTTCTGGTTCCACTCGCGCTCGAGTTCCTGCACGCGCCGCCGCTCCCAGCCCTCTT
GGCTTCGGCCGCGGTGCTCGTCCCGCTCGCTGCTCTGCTCGGTCGAGCAACGGAGGAAGCAGCGGCGCACACCGGCCCGC
TGGTCGGGGGACTACTCGATTCGACGCTCGGCAAGGCTGCCGAGCTGATCATCGCGTTGGCGGCCTTGCGGGCGGACCTT
CACGAGTTGGTGAAGGCCTCGCTTGCTGGTTCCATCCTCCTGCTCATACTCGGAGCCTGTCTTCTCGTCGGCGGACTCCG
TCATGGTCTCCAGCAATTCGATGCCCAGCTTGCCGGAATCAGCGCGCCGATGATGATGCTCGCCGTCGTGCTTCTGCTCG
CCCCCGGGCTTTTCACCTTGGGACCGACCCCCGTGCAGGGTGAGGCGGTCGAGTGGATGAGCGTCGGCCTCGCCCTCGTC
GTCGCCGGGCTTTACGCCCTCTCTTTGCTCGCCACTCTGCGCCTTCCAGGGACGGTTACCGAGCATGAGCACACTGCATG
GCCACTGCGGCGAGCAGCCCTCGTCCTCGCCGGTGCAACGATCGGCCTCCTGTTCATGAGCGAGGTTCTCGTCGGCGCAG
TCGAGCCGGTCGTCGCAGCGACTGGCATCTTCGCGTTCTTCGGCGGAGTCGTGCTGGTACTGCTCGTCGAAAGTGTTGCG
GAGCATTTCGTCGCGGTGCAGGTGGCGCATCGGAACCGCATGGACTCCAGTCTGGGAATCGGCTATGGATCGACCTTGCA
AATCGCGCTCGCCGTGATGTCCATTCTCGTTTTCGCTCGTCTCGTGCCGAGCCGGCCGCTATCGCTCGTCTTCGATGTCT
CTGAACTCGTCGCCCTGCTCGGCGCAGCGCTGGTGTCGACACTCATCGCGCTCGCACTGGCTGGAAGGAGTGATCCTGCC
TGCGGTTTACGCTGGCCTGGCCGTGGGCTGTTCCTTCTTGCCATGCTCGCTGCTCTTCGAGTACCGGGCCAGGGCACCGT
CTGCGTGTCTGACCCGTATCGGTCGGCACGTGTGTCGACTCGGGTTCCCGACACGTCTTCGTACCGGGAGCTCCCGGTTT
CTCATTCGCGCCTCGAGCTTGGAGCGCGCACCGGTTGGACGAGTATAGGTGAAGCGGATCGACCCTTCGGCGCAGCATGG
GCGAGCAGCCAGTCGATTGTCCGAGCCGCCAGGCACCATCGGAGGAGGGAGCGATGGTCGAGGTTCTCGCCGACGAGCAG
ACTGCCCAGACACTCCTGCGAGACTTGCACGACGTCGGCGCACTGTTGA

Upstream 100 bases:

>100_bases
TGCACTCGTGCGAGCTCTGCTCGGCGGCGGCACGAGCTTTTCCGTCCAACCGCTCCCCGCCTACCGGACGCGCGAAGCTG
TCCTCGCCTGAGGGGGGATC

Downstream 100 bases:

>100_bases
CAGGGCATTTTCTCCTCGCCTCCGGCCGTCACAGTGATCGCTATATCGAAAAGTTCGCTCTGCTCCGCTGGCCGCGCCGC
GTGGAAGAGATGTGTCGCGA

Product: vacuolar cation/proton exchanger 1a

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MRRWLAGLLLVPLALEFLHAPPLPALLASAAVLVPLAALLGRATEEAAAHTGPLVGGLLDSTLGKAAELIIALAALRADL
HELVKASLAGSILLLILGACLLVGGLRHGLQQFDAQLAGISAPMMMLAVVLLLAPGLFTLGPTPVQGEAVEWMSVGLALV
VAGLYALSLLATLRLPGTVTEHEHTAWPLRRAALVLAGATIGLLFMSEVLVGAVEPVVAATGIFAFFGGVVLVLLVESVA
EHFVAVQVAHRNRMDSSLGIGYGSTLQIALAVMSILVFARLVPSRPLSLVFDVSELVALLGAALVSTLIALALAGRSDPA
CGLRWPGRGLFLLAMLAALRVPGQGTVCVSDPYRSARVSTRVPDTSSYRELPVSHSRLELGARTGWTSIGEADRPFGAAW
ASSQSIVRAARHHRRRERWSRFSPTSRLPRHSCETCTTSAHC

Sequences:

>Translated_442_residues
MRRWLAGLLLVPLALEFLHAPPLPALLASAAVLVPLAALLGRATEEAAAHTGPLVGGLLDSTLGKAAELIIALAALRADL
HELVKASLAGSILLLILGACLLVGGLRHGLQQFDAQLAGISAPMMMLAVVLLLAPGLFTLGPTPVQGEAVEWMSVGLALV
VAGLYALSLLATLRLPGTVTEHEHTAWPLRRAALVLAGATIGLLFMSEVLVGAVEPVVAATGIFAFFGGVVLVLLVESVA
EHFVAVQVAHRNRMDSSLGIGYGSTLQIALAVMSILVFARLVPSRPLSLVFDVSELVALLGAALVSTLIALALAGRSDPA
CGLRWPGRGLFLLAMLAALRVPGQGTVCVSDPYRSARVSTRVPDTSSYRELPVSHSRLELGARTGWTSIGEADRPFGAAW
ASSQSIVRAARHHRRRERWSRFSPTSRLPRHSCETCTTSAHC
>Mature_442_residues
MRRWLAGLLLVPLALEFLHAPPLPALLASAAVLVPLAALLGRATEEAAAHTGPLVGGLLDSTLGKAAELIIALAALRADL
HELVKASLAGSILLLILGACLLVGGLRHGLQQFDAQLAGISAPMMMLAVVLLLAPGLFTLGPTPVQGEAVEWMSVGLALV
VAGLYALSLLATLRLPGTVTEHEHTAWPLRRAALVLAGATIGLLFMSEVLVGAVEPVVAATGIFAFFGGVVLVLLVESVA
EHFVAVQVAHRNRMDSSLGIGYGSTLQIALAVMSILVFARLVPSRPLSLVFDVSELVALLGAALVSTLIALALAGRSDPA
CGLRWPGRGLFLLAMLAALRVPGQGTVCVSDPYRSARVSTRVPDTSSYRELPVSHSRLELGARTGWTSIGEADRPFGAAW
ASSQSIVRAARHHRRRERWSRFSPTSRLPRHSCETCTTSAHC

Specific function: Unknown

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320075, Length=367, Percent_Identity=30.5177111716621, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004798
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 46415; Mature: 46415

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRWLAGLLLVPLALEFLHAPPLPALLASAAVLVPLAALLGRATEEAAAHTGPLVGGLLD
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHH
STLGKAAELIIALAALRADLHELVKASLAGSILLLILGACLLVGGLRHGLQQFDAQLAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SAPMMMLAVVLLLAPGLFTLGPTPVQGEAVEWMSVGLALVVAGLYALSLLATLRLPGTVT
CHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EHEHTAWPLRRAALVLAGATIGLLFMSEVLVGAVEPVVAATGIFAFFGGVVLVLLVESVA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHFVAVQVAHRNRMDSSLGIGYGSTLQIALAVMSILVFARLVPSRPLSLVFDVSELVALL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GAALVSTLIALALAGRSDPACGLRWPGRGLFLLAMLAALRVPGQGTVCVSDPYRSARVST
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCC
RVPDTSSYRELPVSHSRLELGARTGWTSIGEADRPFGAAWASSQSIVRAARHHRRRERWS
CCCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RFSPTSRLPRHSCETCTTSAHC
CCCCHHCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MRRWLAGLLLVPLALEFLHAPPLPALLASAAVLVPLAALLGRATEEAAAHTGPLVGGLLD
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHH
STLGKAAELIIALAALRADLHELVKASLAGSILLLILGACLLVGGLRHGLQQFDAQLAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SAPMMMLAVVLLLAPGLFTLGPTPVQGEAVEWMSVGLALVVAGLYALSLLATLRLPGTVT
CHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EHEHTAWPLRRAALVLAGATIGLLFMSEVLVGAVEPVVAATGIFAFFGGVVLVLLVESVA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHFVAVQVAHRNRMDSSLGIGYGSTLQIALAVMSILVFARLVPSRPLSLVFDVSELVALL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GAALVSTLIALALAGRSDPACGLRWPGRGLFLLAMLAALRVPGQGTVCVSDPYRSARVST
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCC
RVPDTSSYRELPVSHSRLELGARTGWTSIGEADRPFGAAWASSQSIVRAARHHRRRERWS
CCCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RFSPTSRLPRHSCETCTTSAHC
CCCCHHCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]