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The map label for this gene is prfA [H]

Identifier: 221632126

GI number: 221632126

Start: 101883

End: 102974

Strand: Reverse

Name: prfA [H]

Synonym: trd_0091

Alternate gene names: 221632126

Gene position: 102974-101883 (Counterclockwise)

Preceding gene: 221632127

Following gene: 221632125

Centisome position: 5.14

GC content: 61.63

Gene sequence:

>1092_bases
GTGACGACTCAGACGGTATCCGTACTCGACAAACTCGCCGAACTCGAGCAGCGCTTCGATGAGGTGGAACGACAGCTCGC
CGATCCGGACGTCGCCACCGATCCTCAGCGCGTGGCACAACTGGCACGCGAGCGAGCGGAACTCGAGGAGATCGTCGCGC
TGTACCGCCAGCTCAAGGATATCGACCGGCAAGTCGCTCAAGCGGAAGAACTCCTGGAGGCCGAGGATCCCGATCTGGTG
GAACTGGCTGCCGAGGAGATCGACCGGCTGCGCGAGTACCGCGAGACGATCTATCAGGAGCTGCGGCGTCGTCTCGTCCC
GCGCGATCCGAACGACGAGAAAGACGTCATCGTGGAAATCCGAGCGGGTACTGGAGGCGAAGAGGCTGCGCTCTTCGCGG
CCGATCTTTTCCGCATGTATACCCGCTACGCGGAGCGACAGGGTTGGAAGGTGGAGGTGCTCTCCAGTAGCCCGACCGAA
CTCGGAGGATTCAAGGAGATCATCTTCGAGGTGCGGGGCAAGGGGGCCTACAGCCACTTCAAGCACGAGAGCGGTGTCCA
CCGCGTTCAGCGCGTTCCGATCACCGAATCGTCCGGCCGCATCCATACCTCGACGGCGACCGTCGCCGTGCTTCCCGAAG
CAGACGAGGTCGAAGTGCACATCGACGAAGACGACTTGCGCATCGAAGTCTTCCGTTCCAGTGGACACGGTGGACAGAGC
GTCAATACGACCGACTCAGCCGTGCGGATCACGCACCTTCCGACTGGAATCGTCGTGAGCTGCCAAGACGAGCGGTCGCA
GCTCAAGAATCGTCTCAAGGCGATGGCGGTCCTCCGGGCACGGCTCTACGAACTCCAACGACGCAAGCTCGAAGAGGAGC
GGACCGGGTTGCGCCGGCAGCAGGTCGGCACAGGTGAACGGGCAGAAAAGATCCGCACGTACAACTTCCCCCAAGACCGT
GTGACCGATCATCGTCTCAAGCTGACGGTCAACAACCTGCCAGCCGTACTGGACGGCGAGATCGATATCTTCATTCAGGA
ACTGCAAGCGCTGGAAATGGCCGAACGCCTGCGAGCAGCTGGCATCGACTGA

Upstream 100 bases:

>100_bases
CACGGGCTGGCGCCACGATCAGCGAGGTCGCCACACCAGAGGAAGCAGTCAACCAGGTCCTCGCTGTCCTCAGCGTTCAA
CCCACCGGAGGAGCAGAGCT

Downstream 100 bases:

>100_bases
GCGGGCCCGTGCTCACGTTGCGCGACGTCTTGCGCCGAGCGACCGAGCGGCTGCGCCAGAGCGGCAGTCCCACGCCGCGG
CTGGATGCCGAAGTGCTCCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTTQTVSVLDKLAELEQRFDEVERQLADPDVATDPQRVAQLARERAELEEIVALYRQLKDIDRQVAQAEELLEAEDPDLV
ELAAEEIDRLREYRETIYQELRRRLVPRDPNDEKDVIVEIRAGTGGEEAALFAADLFRMYTRYAERQGWKVEVLSSSPTE
LGGFKEIIFEVRGKGAYSHFKHESGVHRVQRVPITESSGRIHTSTATVAVLPEADEVEVHIDEDDLRIEVFRSSGHGGQS
VNTTDSAVRITHLPTGIVVSCQDERSQLKNRLKAMAVLRARLYELQRRKLEEERTGLRRQQVGTGERAEKIRTYNFPQDR
VTDHRLKLTVNNLPAVLDGEIDIFIQELQALEMAERLRAAGID

Sequences:

>Translated_363_residues
MTTQTVSVLDKLAELEQRFDEVERQLADPDVATDPQRVAQLARERAELEEIVALYRQLKDIDRQVAQAEELLEAEDPDLV
ELAAEEIDRLREYRETIYQELRRRLVPRDPNDEKDVIVEIRAGTGGEEAALFAADLFRMYTRYAERQGWKVEVLSSSPTE
LGGFKEIIFEVRGKGAYSHFKHESGVHRVQRVPITESSGRIHTSTATVAVLPEADEVEVHIDEDDLRIEVFRSSGHGGQS
VNTTDSAVRITHLPTGIVVSCQDERSQLKNRLKAMAVLRARLYELQRRKLEEERTGLRRQQVGTGERAEKIRTYNFPQDR
VTDHRLKLTVNNLPAVLDGEIDIFIQELQALEMAERLRAAGID
>Mature_362_residues
TTQTVSVLDKLAELEQRFDEVERQLADPDVATDPQRVAQLARERAELEEIVALYRQLKDIDRQVAQAEELLEAEDPDLVE
LAAEEIDRLREYRETIYQELRRRLVPRDPNDEKDVIVEIRAGTGGEEAALFAADLFRMYTRYAERQGWKVEVLSSSPTEL
GGFKEIIFEVRGKGAYSHFKHESGVHRVQRVPITESSGRIHTSTATVAVLPEADEVEVHIDEDDLRIEVFRSSGHGGQSV
NTTDSAVRITHLPTGIVVSCQDERSQLKNRLKAMAVLRARLYELQRRKLEEERTGLRRQQVGTGERAEKIRTYNFPQDRV
TDHRLKLTVNNLPAVLDGEIDIFIQELQALEMAERLRAAGID

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=305, Percent_Identity=47.8688524590164, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI34577120, Length=348, Percent_Identity=40.2298850574713, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI166795305, Length=206, Percent_Identity=47.0873786407767, Blast_Score=191, Evalue=9e-49,
Organism=Escherichia coli, GI1787462, Length=353, Percent_Identity=49.5750708215297, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=40, Blast_Score=232, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17542784, Length=286, Percent_Identity=41.2587412587413, Blast_Score=197, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=348, Percent_Identity=40.5172413793103, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI19921226, Length=336, Percent_Identity=43.75, Blast_Score=261, Evalue=8e-70,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41433; Mature: 41302

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQTVSVLDKLAELEQRFDEVERQLADPDVATDPQRVAQLARERAELEEIVALYRQLKD
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IDRQVAQAEELLEAEDPDLVELAAEEIDRLREYRETIYQELRRRLVPRDPNDEKDVIVEI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
RAGTGGEEAALFAADLFRMYTRYAERQGWKVEVLSSSPTELGGFKEIIFEVRGKGAYSHF
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHHHH
KHESGVHRVQRVPITESSGRIHTSTATVAVLPEADEVEVHIDEDDLRIEVFRSSGHGGQS
HHHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCC
VNTTDSAVRITHLPTGIVVSCQDERSQLKNRLKAMAVLRARLYELQRRKLEEERTGLRRQ
CCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
QVGTGERAEKIRTYNFPQDRVTDHRLKLTVNNLPAVLDGEIDIFIQELQALEMAERLRAA
HCCCCHHHHHHHCCCCCHHHCCCCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHC
GID
CCC
>Mature Secondary Structure 
TTQTVSVLDKLAELEQRFDEVERQLADPDVATDPQRVAQLARERAELEEIVALYRQLKD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IDRQVAQAEELLEAEDPDLVELAAEEIDRLREYRETIYQELRRRLVPRDPNDEKDVIVEI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
RAGTGGEEAALFAADLFRMYTRYAERQGWKVEVLSSSPTELGGFKEIIFEVRGKGAYSHF
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHHHH
KHESGVHRVQRVPITESSGRIHTSTATVAVLPEADEVEVHIDEDDLRIEVFRSSGHGGQS
HHHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCC
VNTTDSAVRITHLPTGIVVSCQDERSQLKNRLKAMAVLRARLYELQRRKLEEERTGLRRQ
CCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
QVGTGERAEKIRTYNFPQDRVTDHRLKLTVNNLPAVLDGEIDIFIQELQALEMAERLRAA
HCCCCHHHHHHHCCCCCHHHCCCCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHC
GID
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA