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The map label for this gene is dnaA [H]

Identifier: 22127981

GI number: 22127981

Start: 4565119

End: 4566519

Strand: Reverse

Name: dnaA [H]

Synonym: y4113

Alternate gene names: 22127981

Gene position: 4566519-4565119 (Counterclockwise)

Preceding gene: 22127985

Following gene: 22127980

Centisome position: 99.26

GC content: 48.04

Gene sequence:

>1401_bases
GTGGAGTCCGCCGTGTCACTTTCGCTTTGGCAGCAGTGTCTTGCCCGATTGCAGGATGAGTTACCTGCCACAGAATTTAG
TATGTGGATACGCCCCCTACAGGCGGAACTGAGTGACAATACTCTGGCGCTTTACGCACCTAACCGTTTTGTACTGGACT
GGGTCCGTGATAAGTACTTAAACAATATCAATGGCTTACTTAATGATTTTTGTGGCACCGAGGTCCCTTTACTGCGTTTT
GAGGTAGGGAGTAAACCTGCGGCAAGAGCACACAATAATCCAGTTACAGCCAGCGTGAGTGCGCCTGTTGCTCCGGTTAC
CCGCAGTGCACCTATGCGCCCGAGCTGGGACAACTCACCTGCTCAGCCTGAACTTTCTTATCGTTCGAATGTGAATCCAA
AACACACATTTGATAACTTTGTTGAAGGTAAATCTAACCAGTTGGCCCGCGCTGCCGCCCGTCAGGTAGCCGATAACCCA
GGTGGTGCCTATAACCCGTTATTTCTTTATGGCGGAACAGGCTTGGGTAAAACCCATCTGCTACACGCGGTGGGGAATGG
CATTATGGCCCGTAAAGCCAATGCTAAAGTGGTCTATATGCATTCCGAGCGTTTTGTGCAAGATATGGTCAAAGCCTTGC
AAAATAATGCTATCGAAGAGTTTAAACGCTATTATCGTTCGGTTGATGCTTTATTAATCGATGATATTCAATTTTTTGCC
AATAAAGAGCGTTCTCAAGAAGAGTTTTTCCACACCTTTAATGCGTTGCTAGAGGGTAATCAGCAGATCATTTTGACCTC
AGATCGCTATCCAAAAGAGATTAACGGGGTGGAAGATCGTCTGAAGTCGCGTTTTGGTTGGGGGCTGACGGTCGCCATTG
AGCCACCAGAGCTGGAAACGCGTGTAGCGATCCTAATGAAGAAAGCGGATGAAAATGATATTCGCTTACCAGGAGAGGTC
GCGTTCTTTATTGCCAAGCGTCTGCGTTCCAATGTGCGTGAATTGGAAGGGGCATTGAACCGTGTGATCGCCAATGCCAA
TTTTACTGGCCGCGCTATCACGATTGATTTTGTTCGCGAAGCCCTGCGTGATTTGTTGGCGTTGCAAGAAAAGCTGGTCA
CTATCGATAATATTCAAAAGACTGTTGCTGAATATTATAAAATTAAGGTCGCTGACCTGCTCTCCAAACGGCGTTCCCGT
TCGGTTGCCCGCCCTCGCCAAATGGCTATGGCATTGGCAAAAGAGCTAACCAACCACAGCCTGCCTGAAATCGGCGATGC
GTTTGGTGGCCGCGACCATACCACGGTGTTGCACGCTTGCCGGAAGATTGAGCAATTGCGTGAGGAAAGTCACGACATCA
AAGAAGATTTCTCTAACTTAATCAGAACATTATCCTCCTAG

Upstream 100 bases:

>100_bases
AGTTCGCATGTCACCTTGGCTTGCTACAGGCCATATGGCGTGTGTCAAAACGATGAGTTACAAAATATCCTGATTTCTTT
CTTTTATTGATCTTGTTCGA

Downstream 100 bases:

>100_bases
CGCTATGAAATTTATCATTGAACGTGAGCATCTGCTAAAACCACTGCAACAGGTCAGTAGCCCGCTGGGTGGACGCCCTA
CGTTGCCTATTTTGGGTAAC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MESAVSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNINGLLNDFCGTEVPLLRF
EVGSKPAARAHNNPVTASVSAPVAPVTRSAPMRPSWDNSPAQPELSYRSNVNPKHTFDNFVEGKSNQLARAAARQVADNP
GGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKANAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFA
NKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEV
AFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSR
SVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS

Sequences:

>Translated_466_residues
MESAVSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNINGLLNDFCGTEVPLLRF
EVGSKPAARAHNNPVTASVSAPVAPVTRSAPMRPSWDNSPAQPELSYRSNVNPKHTFDNFVEGKSNQLARAAARQVADNP
GGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKANAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFA
NKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEV
AFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSR
SVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS
>Mature_466_residues
MESAVSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNINGLLNDFCGTEVPLLRF
EVGSKPAARAHNNPVTASVSAPVAPVTRSAPMRPSWDNSPAQPELSYRSNVNPKHTFDNFVEGKSNQLARAAARQVADNP
GGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKANAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFA
NKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEV
AFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSR
SVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=468, Percent_Identity=91.6666666666667, Blast_Score=872, Evalue=0.0,
Organism=Escherichia coli, GI226510964, Length=249, Percent_Identity=28.1124497991968, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 52563; Mature: 52563

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESAVSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
NNINGLLNDFCGTEVPLLRFEVGSKPAARAHNNPVTASVSAPVAPVTRSAPMRPSWDNSP
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
AQPELSYRSNVNPKHTFDNFVEGKSNQLARAAARQVADNPGGAYNPLFLYGGTGLGKTHL
CCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHH
LHAVGNGIMARKANAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFA
HHHHCCCCEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELET
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCHHHHHHHHCCCCEEEEECCCCHHH
RVAILMKKADENDIRLPGEVAFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVRE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHH
ALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSRSVARPRQMAMALAKELTNHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
LPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MESAVSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
NNINGLLNDFCGTEVPLLRFEVGSKPAARAHNNPVTASVSAPVAPVTRSAPMRPSWDNSP
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
AQPELSYRSNVNPKHTFDNFVEGKSNQLARAAARQVADNPGGAYNPLFLYGGTGLGKTHL
CCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHH
LHAVGNGIMARKANAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFA
HHHHCCCCEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELET
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCHHHHHHHHCCCCEEEEECCCCHHH
RVAILMKKADENDIRLPGEVAFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVRE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHH
ALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSRSVARPRQMAMALAKELTNHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
LPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA