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The map label for this gene is tag [H]

Identifier: 22127960

GI number: 22127960

Start: 4541328

End: 4541900

Strand: Direct

Name: tag [H]

Synonym: y4091

Alternate gene names: 22127960

Gene position: 4541328-4541900 (Clockwise)

Preceding gene: 22127952

Following gene: 22127961

Centisome position: 98.71

GC content: 47.82

Gene sequence:

>573_bases
ATGAGCTTGCAACGCTGTGGTTGGGTTACCTCAGATCCGCTTTATCTGGCTTACCACGACACCGAATGGGGGGTGCCACT
CAAAGATAGCCGGGCATTGTTTGAAATGATTTGTCTGGAAGGGCAACAAGCGGGGCTGTCATGGATAACCGTGCTCAAAA
AACGGGAGCATTACCGCAAAAGCTTCCATCATTTTGATCCGGTTAAGGTGGCGAAAATGGGGCCTGAAGAGGTAGAAATA
CGGGTACAAGACAGCGGAATTATCCGCCATCGCGGTAAAATCCAAGCCATCATTACCAACGCACAGGCTTATTTAGCGAT
GGAAGCCAACGGCGAAGACTTCTCACGCTTTATCTGGAGTTTTGTTGATGATGAGCCAAAAATTAACCATTGGTGGTGTC
TGGCAGAAGCCCCGGCAACAACCGCCGTCTCTGATGCCATGTCTAAGGCCTTGAAAAAATGCGGATTTAAATTCATTGGA
TCGACGATTTGTTATGCTTTTATGCAGGCCAGCGGCTTGGTGAACGATCACATGGCAAATTGTTTTTGCCACCCAGATAA
TGCAGTGAAATGA

Upstream 100 bases:

>100_bases
ATCATCCTCCGCTATCACAAGCGACACCGATGCTATAACAAGCGACAAACGCTATAACAAACGACAAGCACCATAACAAA
CAACAAAAAGGTAGATAAAT

Downstream 100 bases:

>100_bases
TTCGAGTCTACCAACCCGACGATCTCAATGCCCTGATGCAACTGTGGCTAACCAGTACTATTGACGCGCACCCTTTTGTT
GCCGAGCAATACTGGTATGA

Product: DNA-3-methyladenine glycosylase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MSLQRCGWVTSDPLYLAYHDTEWGVPLKDSRALFEMICLEGQQAGLSWITVLKKREHYRKSFHHFDPVKVAKMGPEEVEI
RVQDSGIIRHRGKIQAIITNAQAYLAMEANGEDFSRFIWSFVDDEPKINHWWCLAEAPATTAVSDAMSKALKKCGFKFIG
STICYAFMQASGLVNDHMANCFCHPDNAVK

Sequences:

>Translated_190_residues
MSLQRCGWVTSDPLYLAYHDTEWGVPLKDSRALFEMICLEGQQAGLSWITVLKKREHYRKSFHHFDPVKVAKMGPEEVEI
RVQDSGIIRHRGKIQAIITNAQAYLAMEANGEDFSRFIWSFVDDEPKINHWWCLAEAPATTAVSDAMSKALKKCGFKFIG
STICYAFMQASGLVNDHMANCFCHPDNAVK
>Mature_189_residues
SLQRCGWVTSDPLYLAYHDTEWGVPLKDSRALFEMICLEGQQAGLSWITVLKKREHYRKSFHHFDPVKVAKMGPEEVEIR
VQDSGIIRHRGKIQAIITNAQAYLAMEANGEDFSRFIWSFVDDEPKINHWWCLAEAPATTAVSDAMSKALKKCGFKFIGS
TICYAFMQASGLVNDHMANCFCHPDNAVK

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=185, Percent_Identity=69.1891891891892, Blast_Score=281, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 21561; Mature: 21430

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQRCGWVTSDPLYLAYHDTEWGVPLKDSRALFEMICLEGQQAGLSWITVLKKREHYRK
CCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SFHHFDPVKVAKMGPEEVEIRVQDSGIIRHRGKIQAIITNAQAYLAMEANGEDFSRFIWS
HHCCCCCCCEECCCCCCEEEEECCCCEEEECCEEEEEEECCEEEEEEECCCCHHHHHHHH
FVDDEPKINHWWCLAEAPATTAVSDAMSKALKKCGFKFIGSTICYAFMQASGLVNDHMAN
HHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHH
CFCHPDNAVK
EEECCCCCCC
>Mature Secondary Structure 
SLQRCGWVTSDPLYLAYHDTEWGVPLKDSRALFEMICLEGQQAGLSWITVLKKREHYRK
CCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SFHHFDPVKVAKMGPEEVEIRVQDSGIIRHRGKIQAIITNAQAYLAMEANGEDFSRFIWS
HHCCCCCCCEECCCCCCEEEEECCCCEEEECCEEEEEEECCEEEEEEECCCCHHHHHHHH
FVDDEPKINHWWCLAEAPATTAVSDAMSKALKKCGFKFIGSTICYAFMQASGLVNDHMAN
HHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHH
CFCHPDNAVK
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]