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The map label for this gene is kefB

Identifier: 22127842

GI number: 22127842

Start: 4429888

End: 4431696

Strand: Direct

Name: kefB

Synonym: y3972

Alternate gene names: 22127842

Gene position: 4429888-4431696 (Clockwise)

Preceding gene: 22127841

Following gene: 22127843

Centisome position: 96.29

GC content: 51.41

Gene sequence:

>1809_bases
ATGGAGGGCACAGGGCTACTTACCGCTGTTCTAGTGTTTTTATTCGCTGCTGTCGTTGCGGTCCCTATCGCCCAACGCTT
AGGTATTGGTGCGGTACTGGGTTACCTGATCGCGGGTATTGCCATTGGCCCGTGGGGGCTAGGATTTATTCGCGATGTTG
ACGAAATTCTACATTTTTCTGAGCTAGGCGTCGTCTTCCTGATGTTTATCATTGGGTTGGAGTTGAATCCCGCAAAACTT
TGGCAATTAAGGCGATCTATTTTTGGTGTCGGTGCTGGTCAAGTCGTGATAACGGCCGCGGTACTGGGCGCATTGCTTTA
TTTTACCCAGTTCGCCTGGCAGGCAGCGGTTATCGGTGGCGTAGGTTTGGCGATGTCGTCTACCGCCATGGCATTGCAAC
TGATGCGGGAAAAGGGGATGAACCGCAATGAAGGCGGCCAGCTCGGTTTTTCCGTTCTACTGTTCCAGGATATGGCGGTG
ATCCCCGCGTTAGCCTTGATTCCTATTCTGGCGGGTAACGAAGGGGGAGCCAATGACTGGGTCAAAATTGGCCTCAAAAT
TGCGGCTTTTGCCGGTATGTTGATTGGGGGCCGTTATTTGTTGCGCCCGTTGTTCCGCTACATTGTCGCCTCTGGGGTAC
GTGAGGTTTTTACCGCTGCGGCATTGCTGGTGGTGCTGGGGTCCGCGCTGTTTATGGATGCCCTGGGGCTATCGATGGCA
TTGGGGACATTTATCGCCGGGATATTACTGGCTGAAAGTGAGTTTCAGCACGAATTGGAAATCGCTATCGAGCCGTTTAA
AGGGCTCTTGCTCGGTTTGTTTTTTATTTCTGTCGGCATGGCATTGGACCTCGGCGTGCTGTTTACCCATTTACTGGATG
TACTGCTGGGCGTCTTGGCACTGGTCTTTATCAAAAGTGCCATCTTGTATGGTTTGGCGCGGGTGTTTGGCTTGCGGCGT
TCGGTCCGTTTGCAATTTGCCGGTGTGCTCAGCCAAGGCGGCGAATTTGCTTTCGTCCTGTTTTCCGCGGCGTTCTCTCA
GCGTGTGTTGAATGCAGAACAACTGGCATTGCTATTGGTGGTCGTCACCTTATCGATGATGACTACGCCGTTGCTGATGC
AGGTTATTGACCGTATTTTGGTTCGTCGCTACAACGCGCAGGAAGAGAGTGATGAGAAACCCTTTGTTGAAGATAATGAC
CCACAGGTGATTATTGTCGGCTTCGGGCGATTTGGTCAGGTGATTGGCCGTTTATTGATGGCGAACAAAATGCGCATTAC
CGTACTGGAACGTGATGTCAGCGCGGTCAGTATGATGCGCAAATATGGCTACAAAGTTTACTATGGTGACGCGACCGAGT
TGGAACTGCTACGAGCCGCTGGCGCAGAAAAAGCCAAAGCCATTGTTATCACCTGCAATGAGCCGGAAGACACCATGGCG
CTGGTGCATTTATGCCAGCAACACTTCCCTAATCTGCACATTCTTGCTCGCGCCAGAGGGCGTGTCGAAGCCCATGAACT
GCTCCAAAACGGGGTCAAAGACTTCACGCGTGAAACCTTCTCCAGTGCGCTGGAACTGGGGCGTAAGACATTATTGGAAC
TGGGCATGCATCCACATCAGGCCTACCGGGCGCAACAACATTTTCGTCGCTTGGACATGCGAATGCTACGTGAGCTGATG
CCACAACATCATGGCGATGTGGCACAAATTTCCAGAATTAAAGAAGCTCGCCGTGAGTTGGAAGATATTTTTCAACGAGA
AATGCTACATGAAAGCCGGCAGTTAGATGGGTGGGATGAATACGAATAA

Upstream 100 bases:

>100_bases
TATTTACTGGGCCAGACGCCAAAAGCCGGAAACACTCGCCAGTCACGCACAAGCTTATGTGCAATGGCTGCAGTCACCGC
TCACGAGAGGACTCTGATTT

Downstream 100 bases:

>100_bases
TGTCGAGGACAAACGATGACTATCACTCTTAATAAGACAGTGACACGTAAAAGATTTATTGCCGGAGCCGTCTGCCCGCA
ATGTAATGCCTTGGATACGC

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MEGTGLLTAVLVFLFAAVVAVPIAQRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPAKL
WQLRRSIFGVGAGQVVITAAVLGALLYFTQFAWQAAVIGGVGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAV
IPALALIPILAGNEGGANDWVKIGLKIAAFAGMLIGGRYLLRPLFRYIVASGVREVFTAAALLVVLGSALFMDALGLSMA
LGTFIAGILLAESEFQHELEIAIEPFKGLLLGLFFISVGMALDLGVLFTHLLDVLLGVLALVFIKSAILYGLARVFGLRR
SVRLQFAGVLSQGGEFAFVLFSAAFSQRVLNAEQLALLLVVVTLSMMTTPLLMQVIDRILVRRYNAQEESDEKPFVEDND
PQVIIVGFGRFGQVIGRLLMANKMRITVLERDVSAVSMMRKYGYKVYYGDATELELLRAAGAEKAKAIVITCNEPEDTMA
LVHLCQQHFPNLHILARARGRVEAHELLQNGVKDFTRETFSSALELGRKTLLELGMHPHQAYRAQQHFRRLDMRMLRELM
PQHHGDVAQISRIKEARRELEDIFQREMLHESRQLDGWDEYE

Sequences:

>Translated_602_residues
MEGTGLLTAVLVFLFAAVVAVPIAQRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPAKL
WQLRRSIFGVGAGQVVITAAVLGALLYFTQFAWQAAVIGGVGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAV
IPALALIPILAGNEGGANDWVKIGLKIAAFAGMLIGGRYLLRPLFRYIVASGVREVFTAAALLVVLGSALFMDALGLSMA
LGTFIAGILLAESEFQHELEIAIEPFKGLLLGLFFISVGMALDLGVLFTHLLDVLLGVLALVFIKSAILYGLARVFGLRR
SVRLQFAGVLSQGGEFAFVLFSAAFSQRVLNAEQLALLLVVVTLSMMTTPLLMQVIDRILVRRYNAQEESDEKPFVEDND
PQVIIVGFGRFGQVIGRLLMANKMRITVLERDVSAVSMMRKYGYKVYYGDATELELLRAAGAEKAKAIVITCNEPEDTMA
LVHLCQQHFPNLHILARARGRVEAHELLQNGVKDFTRETFSSALELGRKTLLELGMHPHQAYRAQQHFRRLDMRMLRELM
PQHHGDVAQISRIKEARRELEDIFQREMLHESRQLDGWDEYE
>Mature_602_residues
MEGTGLLTAVLVFLFAAVVAVPIAQRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPAKL
WQLRRSIFGVGAGQVVITAAVLGALLYFTQFAWQAAVIGGVGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAV
IPALALIPILAGNEGGANDWVKIGLKIAAFAGMLIGGRYLLRPLFRYIVASGVREVFTAAALLVVLGSALFMDALGLSMA
LGTFIAGILLAESEFQHELEIAIEPFKGLLLGLFFISVGMALDLGVLFTHLLDVLLGVLALVFIKSAILYGLARVFGLRR
SVRLQFAGVLSQGGEFAFVLFSAAFSQRVLNAEQLALLLVVVTLSMMTTPLLMQVIDRILVRRYNAQEESDEKPFVEDND
PQVIIVGFGRFGQVIGRLLMANKMRITVLERDVSAVSMMRKYGYKVYYGDATELELLRAAGAEKAKAIVITCNEPEDTMA
LVHLCQQHFPNLHILARARGRVEAHELLQNGVKDFTRETFSSALELGRKTLLELGMHPHQAYRAQQHFRRLDMRMLRELM
PQHHGDVAQISRIKEARRELEDIFQREMLHESRQLDGWDEYE

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain

Homologues:

Organism=Homo sapiens, GI157388921, Length=398, Percent_Identity=27.1356783919598, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1789749, Length=602, Percent_Identity=77.9069767441861, Blast_Score=923, Evalue=0.0,
Organism=Escherichia coli, GI1786232, Length=600, Percent_Identity=43.3333333333333, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1786685, Length=545, Percent_Identity=29.9082568807339, Blast_Score=182, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KEFB_YERPA (Q1C2S9)

Other databases:

- EMBL:   CP000308
- RefSeq:   YP_653188.1
- ProteinModelPortal:   Q1C2S9
- SMR:   Q1C2S9
- STRING:   Q1C2S9
- GeneID:   4118472
- GenomeReviews:   CP000308_GR
- KEGG:   ypa:YPA_3281
- eggNOG:   COG0475
- HOGENOM:   HBG428809
- OMA:   LRELMPV
- ProtClustDB:   PRK03659
- BioCyc:   YPES360102:YPA_3281-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01412
- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00932

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N

EC number: NA

Molecular weight: Translated: 66329; Mature: 66329

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS51201 RCK_N

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10aaac70)-; HASH(0x12a83e18)-; HASH(0x13372ea8)-; HASH(0x133b13ec)-; HASH(0x10812bfc)-; HASH(0x12e0b75c)-; HASH(0x12d8cc08)-; HASH(0x13079710)-; HASH(0x13449d70)-; HASH(0x1260e668)-; HASH(0x133bf714)-; HASH(0x123e6b80)-; HASH(0x133ba5e8)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CH
>Mature Secondary Structure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CH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA