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The map label for this gene is outO [H]

Identifier: 22127071

GI number: 22127071

Start: 3515572

End: 3516486

Strand: Reverse

Name: outO [H]

Synonym: y3195

Alternate gene names: 22127071

Gene position: 3516486-3515572 (Counterclockwise)

Preceding gene: 22127072

Following gene: 22127069

Centisome position: 76.43

GC content: 35.74

Gene sequence:

>915_bases
ATGATGATGATGGGTATGTTGCAGGAAAAATTATATTCTTTAAATGAACCACTCGGTCTGATAGAGGTAAACATGATGGA
TATACAGGTTTTTTTTGTGAGCTATTTAATTTTTGGGGCCATGGTAGGCAGCTTTCTTAATGTTTTAATCTATAGATTGC
CTATTATGCTGGCTAATTTATCATCACGATCTGAGTCACACGGTGAAGAAATTAAGATGCGTTCTCATTTGAGAAATATT
AATTTATTTCAACCCGGCTCTTTTTGCCATCATTGCAATGAGTCAATACCAATAAAATATAATATACCGATACTCGGTTG
GATTTTTCTGCGTGGTGCCAGTCGTTGTTGTAACAAGAAGATAAGCACCCGCTATCTATTTATTGAAGTATTAGCTGTCA
TTCAAACACTCTTGGTTCTTATGATTTTTAAAGAGGACTTACTTATCTGCACTAGCTTGGTGCTAATTTGGTCACTTACG
GCATTGGCATTTATAGATTTCGATACCTACCTCCTTCCTGATTGTATGACAATACCTTTGCTGTGGCTTGGTCTTCTTAT
CAATATAGATACGGTTTTTGCACCACTAACCTCTGCAGTACTGGGTGCTGTCAGTGGATACCTTTTTCTTTGGCTATCAT
ATTGGTTGTTTAAAATAGTAAGAGGTGTTGATGGAATGGGCTATGGTGATTTTAAATTAATGGCCGCATTAGGTGCTTGG
TTTGGTGTGAGTGCAGTACCTTTTTTAATATTGTTCTCTTCCTTTTTTGGTCTTGTCGCATATGCTATTTTTTATTTTTT
TGATAAAAAAGATAATGGAAAAGAGATTAATTACATCGCATTTGGCCCTTATATTTCATTGGCGGGTGTTCTATACCTGT
TTCTTGGGAGTCATGTGACTAATTTATTTTCTTAA

Upstream 100 bases:

>100_bases
ATAAATTCAGCTAAATTCTCAAATTTACTTGATGCTATCATTGATGTGTCAAATAAATATGATATTAATGTATCTAGTGC
TGATATATACCTTCACCAAG

Downstream 100 bases:

>100_bases
TAAATGGGGTTAATAGCTCCTCTAACTCCAGACACTTAGTATTTTTAGCAGTCAAATCTGATTTGATATCTTGATATCTT
GATATCTTGATATAGAGATT

Product: prepilin peptidase

Products: NA

Alternate protein names: Pectic enzymes secretion protein outO; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MMMMGMLQEKLYSLNEPLGLIEVNMMDIQVFFVSYLIFGAMVGSFLNVLIYRLPIMLANLSSRSESHGEEIKMRSHLRNI
NLFQPGSFCHHCNESIPIKYNIPILGWIFLRGASRCCNKKISTRYLFIEVLAVIQTLLVLMIFKEDLLICTSLVLIWSLT
ALAFIDFDTYLLPDCMTIPLLWLGLLINIDTVFAPLTSAVLGAVSGYLFLWLSYWLFKIVRGVDGMGYGDFKLMAALGAW
FGVSAVPFLILFSSFFGLVAYAIFYFFDKKDNGKEINYIAFGPYISLAGVLYLFLGSHVTNLFS

Sequences:

>Translated_304_residues
MMMMGMLQEKLYSLNEPLGLIEVNMMDIQVFFVSYLIFGAMVGSFLNVLIYRLPIMLANLSSRSESHGEEIKMRSHLRNI
NLFQPGSFCHHCNESIPIKYNIPILGWIFLRGASRCCNKKISTRYLFIEVLAVIQTLLVLMIFKEDLLICTSLVLIWSLT
ALAFIDFDTYLLPDCMTIPLLWLGLLINIDTVFAPLTSAVLGAVSGYLFLWLSYWLFKIVRGVDGMGYGDFKLMAALGAW
FGVSAVPFLILFSSFFGLVAYAIFYFFDKKDNGKEINYIAFGPYISLAGVLYLFLGSHVTNLFS
>Mature_304_residues
MMMMGMLQEKLYSLNEPLGLIEVNMMDIQVFFVSYLIFGAMVGSFLNVLIYRLPIMLANLSSRSESHGEEIKMRSHLRNI
NLFQPGSFCHHCNESIPIKYNIPILGWIFLRGASRCCNKKISTRYLFIEVLAVIQTLLVLMIFKEDLLICTSLVLIWSLT
ALAFIDFDTYLLPDCMTIPLLWLGLLINIDTVFAPLTSAVLGAVSGYLFLWLSYWLFKIVRGVDGMGYGDFKLMAALGAW
FGVSAVPFLILFSSFFGLVAYAIFYFFDKKDNGKEINYIAFGPYISLAGVLYLFLGSHVTNLFS

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=259, Percent_Identity=37.0656370656371, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789732, Length=133, Percent_Identity=45.1127819548872, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 34430; Mature: 34430

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMMGMLQEKLYSLNEPLGLIEVNMMDIQVFFVSYLIFGAMVGSFLNVLIYRLPIMLANL
CCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSRSESHGEEIKMRSHLRNINLFQPGSFCHHCNESIPIKYNIPILGWIFLRGASRCCNKK
HHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEECCHHHHHHHHHHHHHHHCCH
ISTRYLFIEVLAVIQTLLVLMIFKEDLLICTSLVLIWSLTALAFIDFDTYLLPDCMTIPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHH
LWLGLLINIDTVFAPLTSAVLGAVSGYLFLWLSYWLFKIVRGVDGMGYGDFKLMAALGAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
FGVSAVPFLILFSSFFGLVAYAIFYFFDKKDNGKEINYIAFGPYISLAGVLYLFLGSHVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
NLFS
HHCC
>Mature Secondary Structure
MMMMGMLQEKLYSLNEPLGLIEVNMMDIQVFFVSYLIFGAMVGSFLNVLIYRLPIMLANL
CCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSRSESHGEEIKMRSHLRNINLFQPGSFCHHCNESIPIKYNIPILGWIFLRGASRCCNKK
HHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEECCHHHHHHHHHHHHHHHCCH
ISTRYLFIEVLAVIQTLLVLMIFKEDLLICTSLVLIWSLTALAFIDFDTYLLPDCMTIPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHH
LWLGLLINIDTVFAPLTSAVLGAVSGYLFLWLSYWLFKIVRGVDGMGYGDFKLMAALGAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
FGVSAVPFLILFSSFFGLVAYAIFYFFDKKDNGKEINYIAFGPYISLAGVLYLFLGSHVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
NLFS
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8326859; 8065262 [H]