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The map label for this gene is galR [H]

Identifier: 22127059

GI number: 22127059

Start: 3504295

End: 3505386

Strand: Direct

Name: galR [H]

Synonym: y3183

Alternate gene names: 22127059

Gene position: 3504295-3505386 (Clockwise)

Preceding gene: 22127055

Following gene: 161484765

Centisome position: 76.17

GC content: 51.56

Gene sequence:

>1092_bases
TTGCGGGTAATCTTATTTTCCGCAAAACTGAGTGATGTAAGCGGTTACCCTAGGATCTCTGGAACAAAAATGGCCACTAT
AAAGGATGTTGCCAAGCTGGCGGGTGTTTCCGTCGCAACGGTATCTCGTGTTATCAATCATTCTCCCAAAGCCAGTGAAG
CATCACGCGTGGCGGTGTGCAAGGCGATGGAACAACTGCAATACCACCCGAATGCCAACGCCCGAGCACTGGCGCAACAA
TCGACAGAAACGGTCGGTATGATTGTGTCTGATGTCTCGGACCCTTTCTTCGGTGCGATGGTGAAAGCCGTCGAACAAGT
CGCGTATGCCACCGGTAATTTCCTGTTAATTGGCAACGGTTACCATGATGCCGAAAAAGAACGTCAGGCCATCGAACAAC
TCATTCGCCACCGCTGCGCTGCGCTGGTGGTACATGCCAAAAAATTACCCGATGACGAACTGACGTCATTAATGGAACAA
ATTCCTGGCATGGTGTTAATTAACCGCACCTTACCGGGCTTCGAACCCCGTTGTATCGCATTAGATGACCGCTATGGTGC
CTGGCTGGCAACTCGCCATCTCATCCAGCAGGGGCATAAACGGGTCGCGTTCATTTGCTCCAATCATCAGATTTCCGATG
CGCTTGACCGGATGCAAGGCTATTTGGATGCGTTGAAAGAATTTGATATCCCGGTTGATGAGCGTTTAATTACCTACGGC
ACCCCCGACGAACTCGGCGGTGAGCAGGCAATGACCGATCTACTTGGCCGTGGTAAACACTTCACCGCGGTAAGCTGTTA
TAACGACTCAATGGCGGCCGGGGCGTTATCGGTTCTCAGTGATAACAGTATTGATGTGCCACAGGAGATTTCACTCATCG
GTTTTGATGATGTATTAATCTCCCGTTACCTGCGCCCACGCCTGACGACAATCCGTTACCCAGTCGTTGCCATGTCTACC
CAGGCCGCTGAGTTGGCACTCGCATTAGCCAACAACACACCGTTACCCGAAATTACCAATATGTTCAGCCCAACACTGGT
TCGCCGCCACTCTGTGGCCAGCCCGCCAAGCCTACGGGATGACACTGATTAA

Upstream 100 bases:

>100_bases
AGCCATTTTGGGCGATGGCGCGATAGCAAACAAACGCGGCAAGCCATTCTGCTCATCTCCTTTTCTTTGGTTTTTCTTAC
TAAGTTGCGACCATCCCCCC

Downstream 100 bases:

>100_bases
ACGCGTTCCAGGTCAATCAATTCTTCAATGGTTTGACGGCGGCGGATCAAGCGCGGTTGGCCCTTTTCAAACAGTACTTC
AGGTAATAACGGGCGGCTGT

Product: DNA-binding transcriptional regulator GalR

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MRVILFSAKLSDVSGYPRISGTKMATIKDVAKLAGVSVATVSRVINHSPKASEASRVAVCKAMEQLQYHPNANARALAQQ
STETVGMIVSDVSDPFFGAMVKAVEQVAYATGNFLLIGNGYHDAEKERQAIEQLIRHRCAALVVHAKKLPDDELTSLMEQ
IPGMVLINRTLPGFEPRCIALDDRYGAWLATRHLIQQGHKRVAFICSNHQISDALDRMQGYLDALKEFDIPVDERLITYG
TPDELGGEQAMTDLLGRGKHFTAVSCYNDSMAAGALSVLSDNSIDVPQEISLIGFDDVLISRYLRPRLTTIRYPVVAMST
QAAELALALANNTPLPEITNMFSPTLVRRHSVASPPSLRDDTD

Sequences:

>Translated_363_residues
MRVILFSAKLSDVSGYPRISGTKMATIKDVAKLAGVSVATVSRVINHSPKASEASRVAVCKAMEQLQYHPNANARALAQQ
STETVGMIVSDVSDPFFGAMVKAVEQVAYATGNFLLIGNGYHDAEKERQAIEQLIRHRCAALVVHAKKLPDDELTSLMEQ
IPGMVLINRTLPGFEPRCIALDDRYGAWLATRHLIQQGHKRVAFICSNHQISDALDRMQGYLDALKEFDIPVDERLITYG
TPDELGGEQAMTDLLGRGKHFTAVSCYNDSMAAGALSVLSDNSIDVPQEISLIGFDDVLISRYLRPRLTTIRYPVVAMST
QAAELALALANNTPLPEITNMFSPTLVRRHSVASPPSLRDDTD
>Mature_363_residues
MRVILFSAKLSDVSGYPRISGTKMATIKDVAKLAGVSVATVSRVINHSPKASEASRVAVCKAMEQLQYHPNANARALAQQ
STETVGMIVSDVSDPFFGAMVKAVEQVAYATGNFLLIGNGYHDAEKERQAIEQLIRHRCAALVVHAKKLPDDELTSLMEQ
IPGMVLINRTLPGFEPRCIALDDRYGAWLATRHLIQQGHKRVAFICSNHQISDALDRMQGYLDALKEFDIPVDERLITYG
TPDELGGEQAMTDLLGRGKHFTAVSCYNDSMAAGALSVLSDNSIDVPQEISLIGFDDVLISRYLRPRLTTIRYPVVAMST
QAAELALALANNTPLPEITNMFSPTLVRRHSVASPPSLRDDTD

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=80.7228915662651, Blast_Score=556, Evalue=1e-160,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=56.1933534743202, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=34.7181008902077, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=34.0175953079179, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1790369, Length=303, Percent_Identity=34.3234323432343, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=31.5151515151515, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787906, Length=320, Percent_Identity=29.0625, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=26.5625, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786540, Length=339, Percent_Identity=26.8436578171091, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=24.7678018575851, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI48994940, Length=312, Percent_Identity=22.4358974358974, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790689, Length=298, Percent_Identity=26.510067114094, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786268, Length=275, Percent_Identity=22.9090909090909, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39721; Mature: 39721

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILFSAKLSDVSGYPRISGTKMATIKDVAKLAGVSVATVSRVINHSPKASEASRVAVC
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHH
KAMEQLQYHPNANARALAQQSTETVGMIVSDVSDPFFGAMVKAVEQVAYATGNFLLIGNG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECC
YHDAEKERQAIEQLIRHRCAALVVHAKKLPDDELTSLMEQIPGMVLINRTLPGFEPRCIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEE
LDDRYGAWLATRHLIQQGHKRVAFICSNHQISDALDRMQGYLDALKEFDIPVDERLITYG
ECCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHCEEEECC
TPDELGGEQAMTDLLGRGKHFTAVSCYNDSMAAGALSVLSDNSIDVPQEISLIGFDDVLI
CCHHCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECHHHHHH
SRYLRPRLTTIRYPVVAMSTQAAELALALANNTPLPEITNMFSPTLVRRHSVASPPSLRD
HHHHCCHHHHEECCCEEECCHHHHHHHHHCCCCCCHHHHHHHCHHHHHHCCCCCCCCCCC
DTD
CCC
>Mature Secondary Structure
MRVILFSAKLSDVSGYPRISGTKMATIKDVAKLAGVSVATVSRVINHSPKASEASRVAVC
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHH
KAMEQLQYHPNANARALAQQSTETVGMIVSDVSDPFFGAMVKAVEQVAYATGNFLLIGNG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECC
YHDAEKERQAIEQLIRHRCAALVVHAKKLPDDELTSLMEQIPGMVLINRTLPGFEPRCIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEE
LDDRYGAWLATRHLIQQGHKRVAFICSNHQISDALDRMQGYLDALKEFDIPVDERLITYG
ECCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHCEEEECC
TPDELGGEQAMTDLLGRGKHFTAVSCYNDSMAAGALSVLSDNSIDVPQEISLIGFDDVLI
CCHHCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECHHHHHH
SRYLRPRLTTIRYPVVAMSTQAAELALALANNTPLPEITNMFSPTLVRRHSVASPPSLRD
HHHHCCHHHHEECCCEEECCHHHHHHHHHCCCCCCHHHHHHHCHHHHHHCCCCCCCCCCC
DTD
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6283521; 9278503; 6350601; 8188660; 8982002 [H]