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The map label for this gene is amiC [H]

Identifier: 22127037

GI number: 22127037

Start: 3472172

End: 3473440

Strand: Reverse

Name: amiC [H]

Synonym: y3161

Alternate gene names: 22127037

Gene position: 3473440-3472172 (Counterclockwise)

Preceding gene: 22127039

Following gene: 22127035

Centisome position: 75.5

GC content: 46.41

Gene sequence:

>1269_bases
TTGTTACTTTGGAGTTACATGGCAGATTCAAATCATAATTCTGGTCGTCGCCGTCTATTACAAGGTGCCGCAGCAGCTTG
GATGCTGAGTATAAGCCGGGTTGGATTTGCCGCATCTTCACATATTGTTGCTGTCCGGGTGTGGCCCTCTTCTACTTACA
CCCGAGTCACATTAGAGTCAAATACACCACTGAAGTACCGCCAATTCGCCTTAACTCATCCCGACCGTATTGTTGTTGAT
ATTGAAGGCGTACAGCTAAATAGCGTATTGAAAGAGATCAGCCGGCAAGTTCAATCCGCTGACCCTTATCTCAAACAGGC
GCGGGTAGGGCAGTTCGATAAAAATACTGTTCGTTTGGTATTGGAACTGAAGCAGAGTATCAGCCCTCAATTATTTACCC
TGAAACCTTTCGCTGAATTCCGTAATCGCTTAGTGGTCGATCTGTACCCTGCGGAGGGGGGCACATCGGCAGAAGATGAT
CCGCTGCTGGCCCTATTGGAAGATTACAATAAAGGTAATGTGGATCGAACCTTGCCACCAGAAACACCCAAAGCAGGTAA
AGCAGGGCGTGACAGACCCATTATTATCATGTTGGACCCAGGACATGGCGGGGAAGATCCTGGGGCTATCGGCAAAAATA
AAACCAAGGAAAAAGACATTGTGCTGCAAATTGCTCGTCGGTTACGGGCGCTTATTCAGAAAGAAGCCAATATGCGGGTG
TTTATGACTCGTAATGAAGATGTCTTTATCCCGCTGAAAGTTCGGGTGGCAAAAGCACGTAAGCTTCGTGCGGATTTATT
CATTTCGATTCATGCCGATGCTTTTACCAATCGGGCGGCTAGCGGCTCCTCGGTCTTTGCTTTATCGACCAAAGGAGCCA
CCAGTACTGCCGCTCGTTTTCTGGCTCAGACACAAAATGAGGCCGATCAGATTGGTGGGGTCAGCAAAAGTGGTGATCCT
TATCTTGACCATACCATTATTGATTTATTGCAAACCGCAACTATCAACGATAGCTTGAAGTTTGGTAAAGAAGTACTGAG
TCGGATGAGTAAAATCAACAAATTGCACAAAAATCGTGTCGATCAAGCAGGATTTGCGGTACTAAAAGCACCTGATATCC
CGTCAATTCTGGTAGAAACTGCGTTCATCAGTAATTTGGAAGAAGAGCGGAAATTACGTACCAGCCGTTTTCAGCAACAG
ATAGCGGAATCTATTTTTGCTGGTATCAAAGCGTACTTTGCTAATGGCGGGGCAATGGCGCGATTGTGA

Upstream 100 bases:

>100_bases
ACTGAGTCACATAAAGATGTTTTTCTGATGACAGCGTTAAATTGATTCGTTAAAGTTTTTCCTCATAATTTTTTTGGTTT
GTTTTTTCATTAGATTTTAG

Downstream 100 bases:

>100_bases
TGGATGTTTCTGTAAAATAATGGGTGTTTCTGTCAAAAATAGAGTGACTATAAATAGCGTGGTTATAGTTCAATACACGA
GGACGAGGCATCCTACCAGG

Product: N-acetylmuramoyl-L-alanine amidase AmiC precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MLLWSYMADSNHNSGRRRLLQGAAAAWMLSISRVGFAASSHIVAVRVWPSSTYTRVTLESNTPLKYRQFALTHPDRIVVD
IEGVQLNSVLKEISRQVQSADPYLKQARVGQFDKNTVRLVLELKQSISPQLFTLKPFAEFRNRLVVDLYPAEGGTSAEDD
PLLALLEDYNKGNVDRTLPPETPKAGKAGRDRPIIIMLDPGHGGEDPGAIGKNKTKEKDIVLQIARRLRALIQKEANMRV
FMTRNEDVFIPLKVRVAKARKLRADLFISIHADAFTNRAASGSSVFALSTKGATSTAARFLAQTQNEADQIGGVSKSGDP
YLDHTIIDLLQTATINDSLKFGKEVLSRMSKINKLHKNRVDQAGFAVLKAPDIPSILVETAFISNLEEERKLRTSRFQQQ
IAESIFAGIKAYFANGGAMARL

Sequences:

>Translated_422_residues
MLLWSYMADSNHNSGRRRLLQGAAAAWMLSISRVGFAASSHIVAVRVWPSSTYTRVTLESNTPLKYRQFALTHPDRIVVD
IEGVQLNSVLKEISRQVQSADPYLKQARVGQFDKNTVRLVLELKQSISPQLFTLKPFAEFRNRLVVDLYPAEGGTSAEDD
PLLALLEDYNKGNVDRTLPPETPKAGKAGRDRPIIIMLDPGHGGEDPGAIGKNKTKEKDIVLQIARRLRALIQKEANMRV
FMTRNEDVFIPLKVRVAKARKLRADLFISIHADAFTNRAASGSSVFALSTKGATSTAARFLAQTQNEADQIGGVSKSGDP
YLDHTIIDLLQTATINDSLKFGKEVLSRMSKINKLHKNRVDQAGFAVLKAPDIPSILVETAFISNLEEERKLRTSRFQQQ
IAESIFAGIKAYFANGGAMARL
>Mature_422_residues
MLLWSYMADSNHNSGRRRLLQGAAAAWMLSISRVGFAASSHIVAVRVWPSSTYTRVTLESNTPLKYRQFALTHPDRIVVD
IEGVQLNSVLKEISRQVQSADPYLKQARVGQFDKNTVRLVLELKQSISPQLFTLKPFAEFRNRLVVDLYPAEGGTSAEDD
PLLALLEDYNKGNVDRTLPPETPKAGKAGRDRPIIIMLDPGHGGEDPGAIGKNKTKEKDIVLQIARRLRALIQKEANMRV
FMTRNEDVFIPLKVRVAKARKLRADLFISIHADAFTNRAASGSSVFALSTKGATSTAARFLAQTQNEADQIGGVSKSGDP
YLDHTIIDLLQTATINDSLKFGKEVLSRMSKINKLHKNRVDQAGFAVLKAPDIPSILVETAFISNLEEERKLRTSRFQQQ
IAESIFAGIKAYFANGGAMARL

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=419, Percent_Identity=70.4057279236277, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1788776, Length=232, Percent_Identity=48.2758620689655, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=41.7777777777778, Blast_Score=190, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46727; Mature: 46727

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLWSYMADSNHNSGRRRLLQGAAAAWMLSISRVGFAASSHIVAVRVWPSSTYTRVTLES
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECC
NTPLKYRQFALTHPDRIVVDIEGVQLNSVLKEISRQVQSADPYLKQARVGQFDKNTVRLV
CCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHH
LELKQSISPQLFTLKPFAEFRNRLVVDLYPAEGGTSAEDDPLLALLEDYNKGNVDRTLPP
HHHHHHCCCCEEEECHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
ETPKAGKAGRDRPIIIMLDPGHGGEDPGAIGKNKTKEKDIVLQIARRLRALIQKEANMRV
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
FMTRNEDVFIPLKVRVAKARKLRADLFISIHADAFTNRAASGSSVFALSTKGATSTAARF
EEECCCCEEEEEEEEEHHHHHHEEEEEEEEECCHHHCCCCCCCEEEEEECCCCHHHHHHH
LAQTQNEADQIGGVSKSGDPYLDHTIIDLLQTATINDSLKFGKEVLSRMSKINKLHKNRV
HHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
DQAGFAVLKAPDIPSILVETAFISNLEEERKLRTSRFQQQIAESIFAGIKAYFANGGAMA
CHHCEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RL
CC
>Mature Secondary Structure
MLLWSYMADSNHNSGRRRLLQGAAAAWMLSISRVGFAASSHIVAVRVWPSSTYTRVTLES
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECC
NTPLKYRQFALTHPDRIVVDIEGVQLNSVLKEISRQVQSADPYLKQARVGQFDKNTVRLV
CCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHH
LELKQSISPQLFTLKPFAEFRNRLVVDLYPAEGGTSAEDDPLLALLEDYNKGNVDRTLPP
HHHHHHCCCCEEEECHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
ETPKAGKAGRDRPIIIMLDPGHGGEDPGAIGKNKTKEKDIVLQIARRLRALIQKEANMRV
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
FMTRNEDVFIPLKVRVAKARKLRADLFISIHADAFTNRAASGSSVFALSTKGATSTAARF
EEECCCCEEEEEEEEEHHHHHHEEEEEEEEECCHHHCCCCCCCEEEEEECCCCHHHHHHH
LAQTQNEADQIGGVSKSGDPYLDHTIIDLLQTATINDSLKFGKEVLSRMSKINKLHKNRV
HHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
DQAGFAVLKAPDIPSILVETAFISNLEEERKLRTSRFQQQIAESIFAGIKAYFANGGAMA
CHHCEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]