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The map label for this gene is bioD [H]

Identifier: 22126905

GI number: 22126905

Start: 3336044

End: 3336781

Strand: Reverse

Name: bioD [H]

Synonym: y3028

Alternate gene names: 22126905

Gene position: 3336781-3336044 (Counterclockwise)

Preceding gene: 22126906

Following gene: 22126903

Centisome position: 72.53

GC content: 53.93

Gene sequence:

>738_bases
ATGGGGTTATCTATCGTGACTAAACGTTGGTTTATCACCGGTACCGATACTGATGTCGGTAAAACCGTCGCCAGTTGTGC
ATTACTGCAAGCGGCCACCGCTCAGGGGTACCGTACTGCTGGCTATAAACCGGTAGCATCCGGTAGCCAGATGACCGCTG
ACGGCTTGCGCAATAGTGATGCCCTGGCGTTGCAGGCGAATAGCAGCCAGAGGCTCGGTTACTCACAAGTGAATCCGTTC
ACTTTTTTGGAAGCGACATCACCGCATATTGCCAGTGAAAGTGAAGGGAGGGCGATCCCTTTGACGGCGTTATCACAGGG
TTTACGTCAACTGGAACCCTCCGCTGACTGGATATTGATAGAAGGGGCCGGCGGCTGGTTTACCCCGCTTTCTCCGCAGG
CCACATTTGCCGATTGGGTACAACAGGAACAATTGCCGGTCATTATGGTGGTTGGGGTGAAACTGGGGTGTATCAACCAT
GCGTTGCTCACGGCACAGGCGATTCAGCACGCCGGGCTGACACTGGCCGGTTGGGTTGCCAATGAGGTGACACCGGCAGG
GAGACGGCAGGCCGAATATCAGGCCACATTGACCCGGATGATAACCGCGCCATTGCTGGGAATTATTCCCTATCTTTCTG
ATATAGAAGAAAATCCGGTGACAACACGCCGTGATCTTGGGCACTACTTGGATTTGACGGTATTACGTGCAGCAGAACGG
GAGGCTGTCAACATGTGA

Upstream 100 bases:

>100_bases
GGGCGTCAGGCTGGGTTACAGGGGCGTCAACGTCTGGCGGCGCTACAACGGGCCTATGTCATGCAATCTGGCGGCTATCC
ACTGAGTTATCATATGGTTT

Downstream 100 bases:

>100_bases
TACATATATGGACGCCGCTACAAGCATGTGATGTGGCTTGCAATCAGAGGCCATCAGGTGCGATTAACCTTATGTAGTTA
ATCTACCCGAGTATGGTTAA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MGLSIVTKRWFITGTDTDVGKTVASCALLQAATAQGYRTAGYKPVASGSQMTADGLRNSDALALQANSSQRLGYSQVNPF
TFLEATSPHIASESEGRAIPLTALSQGLRQLEPSADWILIEGAGGWFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINH
ALLTAQAIQHAGLTLAGWVANEVTPAGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAER
EAVNM

Sequences:

>Translated_245_residues
MGLSIVTKRWFITGTDTDVGKTVASCALLQAATAQGYRTAGYKPVASGSQMTADGLRNSDALALQANSSQRLGYSQVNPF
TFLEATSPHIASESEGRAIPLTALSQGLRQLEPSADWILIEGAGGWFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINH
ALLTAQAIQHAGLTLAGWVANEVTPAGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAER
EAVNM
>Mature_244_residues
GLSIVTKRWFITGTDTDVGKTVASCALLQAATAQGYRTAGYKPVASGSQMTADGLRNSDALALQANSSQRLGYSQVNPFT
FLEATSPHIASESEGRAIPLTALSQGLRQLEPSADWILIEGAGGWFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINHA
LLTAQAIQHAGLTLAGWVANEVTPAGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAERE
AVNM

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=230, Percent_Identity=70.8695652173913, Blast_Score=330, Evalue=7e-92,
Organism=Escherichia coli, GI87081944, Length=230, Percent_Identity=49.5652173913044, Blast_Score=210, Evalue=8e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 26290; Mature: 26159

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLSIVTKRWFITGTDTDVGKTVASCALLQAATAQGYRTAGYKPVASGSQMTADGLRNSD
CCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
ALALQANSSQRLGYSQVNPFTFLEATSPHIASESEGRAIPLTALSQGLRQLEPSADWILI
EEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEE
EGAGGWFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINHALLTAQAIQHAGLTLAGWVA
ECCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEHHH
NEVTPAGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAER
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
EAVNM
HHCCC
>Mature Secondary Structure 
GLSIVTKRWFITGTDTDVGKTVASCALLQAATAQGYRTAGYKPVASGSQMTADGLRNSD
CCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
ALALQANSSQRLGYSQVNPFTFLEATSPHIASESEGRAIPLTALSQGLRQLEPSADWILI
EEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEE
EGAGGWFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINHALLTAQAIQHAGLTLAGWVA
ECCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEHHH
NEVTPAGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAER
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
EAVNM
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]