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The map label for this gene is sseA [H]

Identifier: 22126872

GI number: 22126872

Start: 3302986

End: 3303840

Strand: Direct

Name: sseA [H]

Synonym: y2995

Alternate gene names: 22126872

Gene position: 3302986-3303840 (Clockwise)

Preceding gene: 22126866

Following gene: 22126887

Centisome position: 71.79

GC content: 51.11

Gene sequence:

>855_bases
ATGAACTCAGATTTTCTGGTTACACCGCAATGGCTTGCAGCACATGCTAATGATGCCAATATAGTTATTTTGGATGCCAG
AATGTCGCCTCCCGGAGTGGTCCCCAAACGTAATATTCAGGCTGAGTTTGAACAGGGCCATATTCCCGGCGCGGTCTATT
TTGATATTGATGCAATAGCAGATCACAGTACCGGCTTACCCCATATGCTGCCCTCTCCTCAGCTATTCAGTGAAATGGCA
GGGCAGTTAGGCATCACTGAACAACACACGGTGGTGATTTATGATGAGGGTAATCTGTTTTCTGCACCACGCGTGTGGTG
GACGTTCCGCACATTTGGCGCTAAAAATGTGCGTATTCTGGCAAGTGGTCTGAGCGGGTGGCAACAAGCAGGCTATAAGC
TGGAAAGTGGTCCTGCACACCCTACACCACAAACCTTCAATGTCACTTTCAATGCCGCCGCCGTTTCTAGTGTCGATGAG
GTACTGGCGGTTCTGGGTAATAATGAGGTGCAAATATTGGATGCTCGGCCATCAGCAAGATATAGAGCACAAGAGCCGGA
ACCACGCCCTGGTTTGCGTCTTGGGCGCATCCCTGGCAGCATCAATATACCGTGGGGGACCATGGTGGAGAATGGTCATC
TGAAGTCACCACAGGCATTGGCAGAAATATTTGCGGCTCAAGGGGTGGATTTAACTAAGCCCATTATTACCAGTTGTGGT
TCAGGGGTCACCGCAGCGGTAGTGGTACTGGGTCTAGCGGCGGTGAATGCACGGTCAGTGTCACTCTATGATGGCTCTTG
GGCTGAATGGGGGGCGTCAAACTCTCTACCAATAGACGCAACGCCTCTGGCGTGA

Upstream 100 bases:

>100_bases
ATAAGTCGGGGTTTAATTACAATTATTTCGTTATATAGTGAGTCACCCGATGACGTTATCCTCGGAATTACCCCCTTATA
ACTTTGATAGGTAATATGTT

Downstream 100 bases:

>100_bases
TACCAAAGGCTGGCTCACCACCAGCCACTGACATGACTCATTGTTCATTGTTCATTGTTCATTGTTCATTGTTCATTGTT
CATTGCTCATTGCTCATTGC

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNSDFLVTPQWLAAHANDANIVILDARMSPPGVVPKRNIQAEFEQGHIPGAVYFDIDAIADHSTGLPHMLPSPQLFSEMA
GQLGITEQHTVVIYDEGNLFSAPRVWWTFRTFGAKNVRILASGLSGWQQAGYKLESGPAHPTPQTFNVTFNAAAVSSVDE
VLAVLGNNEVQILDARPSARYRAQEPEPRPGLRLGRIPGSINIPWGTMVENGHLKSPQALAEIFAAQGVDLTKPIITSCG
SGVTAAVVVLGLAAVNARSVSLYDGSWAEWGASNSLPIDATPLA

Sequences:

>Translated_284_residues
MNSDFLVTPQWLAAHANDANIVILDARMSPPGVVPKRNIQAEFEQGHIPGAVYFDIDAIADHSTGLPHMLPSPQLFSEMA
GQLGITEQHTVVIYDEGNLFSAPRVWWTFRTFGAKNVRILASGLSGWQQAGYKLESGPAHPTPQTFNVTFNAAAVSSVDE
VLAVLGNNEVQILDARPSARYRAQEPEPRPGLRLGRIPGSINIPWGTMVENGHLKSPQALAEIFAAQGVDLTKPIITSCG
SGVTAAVVVLGLAAVNARSVSLYDGSWAEWGASNSLPIDATPLA
>Mature_284_residues
MNSDFLVTPQWLAAHANDANIVILDARMSPPGVVPKRNIQAEFEQGHIPGAVYFDIDAIADHSTGLPHMLPSPQLFSEMA
GQLGITEQHTVVIYDEGNLFSAPRVWWTFRTFGAKNVRILASGLSGWQQAGYKLESGPAHPTPQTFNVTFNAAAVSSVDE
VLAVLGNNEVQILDARPSARYRAQEPEPRPGLRLGRIPGSINIPWGTMVENGHLKSPQALAEIFAAQGVDLTKPIITSCG
SGVTAAVVVLGLAAVNARSVSLYDGSWAEWGASNSLPIDATPLA

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=272, Percent_Identity=40.4411764705882, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI61835204, Length=272, Percent_Identity=40.4411764705882, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI194473681, Length=272, Percent_Identity=40.4411764705882, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI17402865, Length=273, Percent_Identity=36.996336996337, Blast_Score=192, Evalue=2e-49,
Organism=Escherichia coli, GI87082121, Length=279, Percent_Identity=50.5376344086022, Blast_Score=297, Evalue=7e-82,
Organism=Caenorhabditis elegans, GI71997283, Length=306, Percent_Identity=25.1633986928105, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17561888, Length=287, Percent_Identity=25.0871080139373, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17559150, Length=253, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543836, Length=296, Percent_Identity=25.3378378378378, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543838, Length=296, Percent_Identity=25.3378378378378, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115534702, Length=207, Percent_Identity=26.5700483091787, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324825, Length=243, Percent_Identity=30.4526748971193, Blast_Score=135, Evalue=8e-33,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30324; Mature: 30324

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSDFLVTPQWLAAHANDANIVILDARMSPPGVVPKRNIQAEFEQGHIPGAVYFDIDAIA
CCCCEEECCHHHEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEHHHC
DHSTGLPHMLPSPQLFSEMAGQLGITEQHTVVIYDEGNLFSAPRVWWTFRTFGAKNVRIL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEECCCEEEEEEEECCCCCEEEE
ASGLSGWQQAGYKLESGPAHPTPQTFNVTFNAAAVSSVDEVLAVLGNNEVQILDARPSAR
EECCCHHHHCCEEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCCC
YRAQEPEPRPGLRLGRIPGSINIPWGTMVENGHLKSPQALAEIFAAQGVDLTKPIITSCG
CCCCCCCCCCCCEEECCCCEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCC
SGVTAAVVVLGLAAVNARSVSLYDGSWAEWGASNSLPIDATPLA
CCHHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSDFLVTPQWLAAHANDANIVILDARMSPPGVVPKRNIQAEFEQGHIPGAVYFDIDAIA
CCCCEEECCHHHEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEHHHC
DHSTGLPHMLPSPQLFSEMAGQLGITEQHTVVIYDEGNLFSAPRVWWTFRTFGAKNVRIL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEECCCEEEEEEEECCCCCEEEE
ASGLSGWQQAGYKLESGPAHPTPQTFNVTFNAAAVSSVDEVLAVLGNNEVQILDARPSAR
EECCCHHHHCCEEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCCC
YRAQEPEPRPGLRLGRIPGSINIPWGTMVENGHLKSPQALAEIFAAQGVDLTKPIITSCG
CCCCCCCCCCCCEEECCCCEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCC
SGVTAAVVVLGLAAVNARSVSLYDGSWAEWGASNSLPIDATPLA
CCHHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]