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The map label for this gene is nfo [H]

Identifier: 22126756

GI number: 22126756

Start: 3180005

End: 3180889

Strand: Direct

Name: nfo [H]

Synonym: y2879

Alternate gene names: 22126756

Gene position: 3180005-3180889 (Clockwise)

Preceding gene: 22126755

Following gene: 22126765

Centisome position: 69.12

GC content: 49.38

Gene sequence:

>885_bases
ATGGCCCGGTTATCAAAGGAGAATGGAATGAAATTTGTCGGTGCACATGTCAGCGCAGCGGGTGGTGTAGATCAAGCGGT
AATTAGAGCGCATGAACTTGAGGCCACGGCCTTTGCGCTGTTTACCAAGAATCAACGTCAATGGCGGGCCGCCCCTTTAG
CGGAAGACGTAATTGAGAAATTTAAACTCGCGTGTGAGAAGTACGGCTATACCTCGGCACAAATTCTGCCTCACGATAGT
TACCTGATTAATCTCGGGCACCCAGTCACCGAGGCACTGGAAAAATCCCGTGAAGCCTTTATCGATGAGTTAGTTCGCTG
CCAGCAACTGGGGTTATCATTACTGAACTTCCATCCCGGTAGCCATTTACTGCAAATTGATGAAGACCAATGCTTGGCGC
GGATTGCCGAATCGATCAACATCGCGTTAGACGCCACTGAAGGCGTGACTGCAGTAATTGAAAATACCGCAGGCCAGGGC
AGTAACCTGGGCTTTAAGTTTGAACATTTAGCCGCCATCATTGAAAGAGTGGAAGATAAAAGCCGCGTCGGCGTCTGTAT
TGATACCTGCCATGCTTTCGCCGCTGGCTATGATTTACGGACTGAAGAAGATTGTGAGCACACCTTCGCGGCATTGGGCA
AGATCGTCGGCTTCCAGTATCTGCGTGGGATGCATCTTAATGATGCGAAAAGCGAATTTAACAGCCGGGTTGACCGCCAC
CACAGCCTGGGTGAAGGCAATATTGGCAAAACCGTATTCAGCTATATTATGCGCGACTCACGTTTCGATAATATCCCATT
GATTCTGGAAACGGTGAATATGGATATCTGGGCCGAAGAGATCGCCTGGCTGAAATCACAGGCAGAGATTGAGCCCTCGT
TGTAA

Upstream 100 bases:

>100_bases
AAACCTCACAGGCTAAAACAAACCGTTTAAGGGCCGTATCCGCGGCCCTTATTTCCCTTCACCGCTTATCCCATTCAATC
ATGTCCCTATCAATGCCATA

Downstream 100 bases:

>100_bases
CGTAGAAAATGCCCGCCAGTAAACCTGGCGGGAATTGAACCTGACGGGAATTGAACCTAACGGGAATTGTGTCATCGCGG
ATGCCTTTAATGGCATACTC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MARLSKENGMKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEKFKLACEKYGYTSAQILPHDS
YLINLGHPVTEALEKSREAFIDELVRCQQLGLSLLNFHPGSHLLQIDEDQCLARIAESINIALDATEGVTAVIENTAGQG
SNLGFKFEHLAAIIERVEDKSRVGVCIDTCHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRH
HSLGEGNIGKTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQAEIEPSL

Sequences:

>Translated_294_residues
MARLSKENGMKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEKFKLACEKYGYTSAQILPHDS
YLINLGHPVTEALEKSREAFIDELVRCQQLGLSLLNFHPGSHLLQIDEDQCLARIAESINIALDATEGVTAVIENTAGQG
SNLGFKFEHLAAIIERVEDKSRVGVCIDTCHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRH
HSLGEGNIGKTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQAEIEPSL
>Mature_293_residues
ARLSKENGMKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEKFKLACEKYGYTSAQILPHDSY
LINLGHPVTEALEKSREAFIDELVRCQQLGLSLLNFHPGSHLLQIDEDQCLARIAESINIALDATEGVTAVIENTAGQGS
NLGFKFEHLAAIIERVEDKSRVGVCIDTCHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRHH
SLGEGNIGKTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQAEIEPSL

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=278, Percent_Identity=81.294964028777, Blast_Score=479, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17531193, Length=267, Percent_Identity=45.6928838951311, Blast_Score=274, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6322735, Length=284, Percent_Identity=41.5492957746479, Blast_Score=229, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 32659; Mature: 32528

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLSKENGMKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEK
CCCCCCCCCCEEEECCHHHCCCHHHHHHHHHHHHHHHHEEEECCCHHHHCCCHHHHHHHH
FKLACEKYGYTSAQILPHDSYLINLGHPVTEALEKSREAFIDELVRCQQLGLSLLNFHPG
HHHHHHHCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCC
SHLLQIDEDQCLARIAESINIALDATEGVTAVIENTAGQGSNLGFKFEHLAAIIERVEDK
CCEEEECHHHHHHHHHHHHEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
SRVGVCIDTCHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRH
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
HSLGEGNIGKTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQAEIEPSL
HCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARLSKENGMKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEK
CCCCCCCCCEEEECCHHHCCCHHHHHHHHHHHHHHHHEEEECCCHHHHCCCHHHHHHHH
FKLACEKYGYTSAQILPHDSYLINLGHPVTEALEKSREAFIDELVRCQQLGLSLLNFHPG
HHHHHHHCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCC
SHLLQIDEDQCLARIAESINIALDATEGVTAVIENTAGQGSNLGFKFEHLAAIIERVEDK
CCEEEECHHHHHHHHHHHHEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
SRVGVCIDTCHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRH
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
HSLGEGNIGKTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQAEIEPSL
HCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA