Click here to switch to the map view.

The map label for this gene is artJ [H]

Identifier: 22126721

GI number: 22126721

Start: 3144507

End: 3145265

Strand: Direct

Name: artJ [H]

Synonym: y2844

Alternate gene names: 22126721

Gene position: 3144507-3145265 (Clockwise)

Preceding gene: 22126710

Following gene: 22126727

Centisome position: 68.35

GC content: 44.53

Gene sequence:

>759_bases
TTGAAAAATTCAGACAGGAGCTTAAGCATGAAAAAATTACTATTGGCAACGTTATTAAGTGGCATGGCTTTCAGCGCTAC
CGCCGCAGAGACCCTCCGTTTTGCAGCCTCAGCCACCTACCCGCCATTTGAATCTATGGATGCCAATAACGAAATCGTTG
GCTTTGATATGGATCTGGCAAAAGCACTGTGTAAACAAATGGAAGCCAACTGTACTTTCACTAATCAGGCTTTTGATAGC
CTGATCCCAGCGCTTAAATTCAAACGTTATGATGCCGTTATCTCTGGTATGGATATCACGCCAGAACGCAGCAAGCAGGT
GGCATTCACTCAGCCTTACTACGCTAACTCCGCGATTGTTATCGCCCCGAAAGGTAAATTCAGCACTTTTGCTGACCTGA
AAGGCAAAAAGATTGGGATGGAGAATGGCACTACCCATCAGAAATATTTACAGGATAAACATCCTGAAATTCAGACCGTG
TCTTATGACAGCTACCAGAATGCCATCATTGATCTGAAAAATGGCCGTATTGATGGTGTGTTTGGTGATACTGCGGTAGT
GAATGAATGGCTGAAAACCAATCCTAATTTAGCCTCTGTCGGCGAGCATGTGACCGACCCACAATATTTCGGCACCGGTT
TAGGTATTGCTGTGCGCCCGGATAACATCGCCCTACTGACAAAATTGAATAAAGCCATTGATGCGGTGAAAGCTGATGGT
ACTTATCAAGCGATCAATGATAAGTGGTTCCCTCAATAA

Upstream 100 bases:

>100_bases
AAATTCATAAAATGGCAATGTAAATCCCATCAGACAGGGGCTTTTTTGGCAAAAAAAACAAACCAGATACAATAAAATCA
ATAACTAATAACATATTCTA

Downstream 100 bases:

>100_bases
TCCCCTTCATATTTGACGTTGCAGGGGGATGCGCTTGTTAGCTGCCGACCTGCAACGCCAGTGACTTTAACGTTTTAACG
CTTTGACTTTGGCTCACTGC

Product: arginine 3rd transport system periplasmic binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKNSDRSLSMKKLLLATLLSGMAFSATAAETLRFAASATYPPFESMDANNEIVGFDMDLAKALCKQMEANCTFTNQAFDS
LIPALKFKRYDAVISGMDITPERSKQVAFTQPYYANSAIVIAPKGKFSTFADLKGKKIGMENGTTHQKYLQDKHPEIQTV
SYDSYQNAIIDLKNGRIDGVFGDTAVVNEWLKTNPNLASVGEHVTDPQYFGTGLGIAVRPDNIALLTKLNKAIDAVKADG
TYQAINDKWFPQ

Sequences:

>Translated_252_residues
MKNSDRSLSMKKLLLATLLSGMAFSATAAETLRFAASATYPPFESMDANNEIVGFDMDLAKALCKQMEANCTFTNQAFDS
LIPALKFKRYDAVISGMDITPERSKQVAFTQPYYANSAIVIAPKGKFSTFADLKGKKIGMENGTTHQKYLQDKHPEIQTV
SYDSYQNAIIDLKNGRIDGVFGDTAVVNEWLKTNPNLASVGEHVTDPQYFGTGLGIAVRPDNIALLTKLNKAIDAVKADG
TYQAINDKWFPQ
>Mature_252_residues
MKNSDRSLSMKKLLLATLLSGMAFSATAAETLRFAASATYPPFESMDANNEIVGFDMDLAKALCKQMEANCTFTNQAFDS
LIPALKFKRYDAVISGMDITPERSKQVAFTQPYYANSAIVIAPKGKFSTFADLKGKKIGMENGTTHQKYLQDKHPEIQTV
SYDSYQNAIIDLKNGRIDGVFGDTAVVNEWLKTNPNLASVGEHVTDPQYFGTGLGIAVRPDNIALLTKLNKAIDAVKADG
TYQAINDKWFPQ

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=243, Percent_Identity=78.6008230452675, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1787088, Length=241, Percent_Identity=68.0497925311203, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=36.7588932806324, Blast_Score=167, Evalue=5e-43,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=38.8646288209607, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1788228, Length=252, Percent_Identity=30.952380952381, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787031, Length=247, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=8e-24,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27685; Mature: 27685

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSDRSLSMKKLLLATLLSGMAFSATAAETLRFAASATYPPFESMDANNEIVGFDMDLA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHH
KALCKQMEANCTFTNQAFDSLIPALKFKRYDAVISGMDITPERSKQVAFTQPYYANSAIV
HHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEECCCEEE
IAPKGKFSTFADLKGKKIGMENGTTHQKYLQDKHPEIQTVSYDSYQNAIIDLKNGRIDGV
EECCCCCCHHHHCCCCEECCCCCCCHHHHHHCCCCCEEEEECCCCCCEEEEECCCEECCE
FGDTAVVNEWLKTNPNLASVGEHVTDPQYFGTGLGIAVRPDNIALLTKLNKAIDAVKADG
ECCHHHHHHHHHCCCCHHHHCCCCCCCHHHCCCCEEEECCCCEEEHHHHHHHHHHHCCCC
TYQAINDKWFPQ
CEEECCCCCCCC
>Mature Secondary Structure
MKNSDRSLSMKKLLLATLLSGMAFSATAAETLRFAASATYPPFESMDANNEIVGFDMDLA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHH
KALCKQMEANCTFTNQAFDSLIPALKFKRYDAVISGMDITPERSKQVAFTQPYYANSAIV
HHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEECCCEEE
IAPKGKFSTFADLKGKKIGMENGTTHQKYLQDKHPEIQTVSYDSYQNAIIDLKNGRIDGV
EECCCCCCHHHHCCCCEECCCCCCCHHHHHHCCCCCEEEEECCCCCCEEEEECCCEECCE
FGDTAVVNEWLKTNPNLASVGEHVTDPQYFGTGLGIAVRPDNIALLTKLNKAIDAVKADG
ECCHHHHHHHHHCCCCHHHHCCCCCCCHHHCCCCEEEECCCCEEEHHHHHHHHHHHCCCC
TYQAINDKWFPQ
CEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]