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The map label for this gene is helD [H]

Identifier: 22126608

GI number: 22126608

Start: 3012749

End: 3014803

Strand: Reverse

Name: helD [H]

Synonym: y2730

Alternate gene names: 22126608

Gene position: 3014803-3012749 (Counterclockwise)

Preceding gene: 22126611

Following gene: 22126605

Centisome position: 65.53

GC content: 48.37

Gene sequence:

>2055_bases
ATGGAACTTAAAGCGACATCTTTTGGCAAACATCTGTCTCAGCATCCCTATAACCGGGTCCGCTTGCTGAATGCAGGCAT
CGAAGTCTGCGGTGATAAACATCAATACCTAATCCCTTTTAACCAACTGGTTAGTATTCAGTGTAAACGAGGTATCGTTT
GGGGCGAGCTTGAATTTGAGTTGCCGCAGCAGAAAGTTGTACGGTTGCACGGTACTGAATGGCAGGAAACACAACATTTC
TATCATTACTTGCTAGGGGTATGGCAGCAATGGAGTGAAGAAATGAGCCAGGTTTGTGCGGAGGTGCTGCATAAACAGGT
GGAGTCAATTAAGCGAATTGAACAGCAGGATAAATGGTTTAAACGCAGTGAATTAGGCCAGATACAAGTCATGATCCGCG
AGGCATTCAGTGCCTTACCAATGCCAGTGGAACGCTTAGCGGCGTTTGAATCCTGTCGAGCAGATTATGAATGCTGCCTG
CGTTGGTTACAGCAAGGTCGTCACAGTGTTGAACGATGTAATCAGCAATGGACAGCCCGTATGTTGGAGGAGTATCAAGA
TTTTTTCCAAAATGTAGAAACTTCGCCCCTGAATGCCTCACAAAGCCGGGCGGTGGTTAACGGTGAAGATTCAGTTTTGG
TGTTAGCCGGCGCGGGTAGCGGTAAAACATCGGTGCTGGTGGCGCGTGCGGGCTGGTTGCTACGTCGTAATGAGGCACTA
CCTGAGCAGATTTTATTGCTGGCATTTGGTCGCCAGGCGGCCGATGAAATGAATAGCCGTATTAAACAACGCTTGGGCGT
GGATAGCATCCAAGCTAAAACTTTTCATGCTCTGGCATTACATATTATCCAGCAAGGTAGCCGCAAGACACCGGTTATCA
GCAAGCTTGAGTCTGATAGCCAGGCCAGAAGGGCGTTGTTAATTCAACATTGGCAGCAACAGTGTAGCGAGAAGAAAGCA
CAGGCCAAAGGGTGGCGAGAGTGGCTAACTGATGAGTTGGAATGGGACGTTGCTGACGGCGATTTCTGGCAGGACAAAAG
GTTGGCTGCGAGGCTGGCAAGTCGCCTTGAACGTTGGGTAGGGTTGATGCGTATGTACGGTGGCAGTCAGGCTGAAATGA
TTGAGCAAGCGAATGACGAGGTGCGTGACCTGTTTCAAAAGCGTATCCGTTTAATGGCACCGTTGCTAAAAGCCTGGAAA
ACGGCATTGAAAGATGAGGGTGCCGTCGATTTTTCTGGGTTGATTCATCAGGCGGTTAACTTGCTGGAGAAAGGGCGCTT
TGTCAGCCCATGGAAGCATGTTTTGGTTGATGAGTTCCAGGATATCTCACCACAAAGGGCGATGTTACTCGCGGCGCTGC
GTAAACAGAATAAGCAAACTTGCCTGTTTGCGGTTGGCGATGATTGGCAGGCTATTTACCGCTTCAGTGGTGCTGAACTT
TCGCTGACAACGGCTTTTAGCGAGCATTTTGGTGAAGGTGCTGAATGTGCACTGGATACGACTTACCGCTTTAATGATCG
GATTGGTGATATTGCGAACCGGTTTATTCAGCAAAACCCTTATCAACTGAAGAAACCACTTAATAGCTTGAGTAAGGGTA
ATAAAAAGTCGGTCATGATTTTACCCGATGAGCAGTTGGAGGCCCTATTAGATAAATTAAGCGGCTTTGTTAAGGACGGC
GAACGTATCCTAATATTGGCGCGTTACCATCATTTACGCCCCGAAGTTTTACAAAAAGCGGCGACGCGGTGGCCAAAGTT
GACTATCGATTTTATGACCATCCATGCCAGCAAAGGGCAGCAGGCTGATTATGTCATTATCGTTGGCTTACATGAGGGTA
ATGATGGGTTCCCAGCCGCGGCGCGGGAATCTGTACTGGAAGATGTGTTGCTACCTCCTCCCGAAGATTTCCCTGATGCT
GAAGAGCGGCGTTTGCTTTATGTCGCAATAACGCGTGCTAAGCACCAAGTGTGGTTACTACAAGATACGGCACGGCCTTC
TATTTTTGTCTCTCAACTCAGTGAATTAGGTGCCCTGGTACAACGTAAGCCCTAG

Upstream 100 bases:

>100_bases
TGTTACCAACTATCGGATACCTGTTGTTTAAACTCAATTATTTTATTTACACTCCATCATGTTACTTCAATTCACTTGAT
ATGGGTTATGGCGCGCAGAT

Downstream 100 bases:

>100_bases
CAAACATAATAAAGCGATGCTCAATACTAAACCCGGTGGTTGAACCACCGGGTGAGTTACCTATTTTAGGTCCAGGCGCT
GTTGCATGTAACGGTCATAA

Product: DNA helicase IV

Products: NA

Alternate protein names: 75 kDa helicase [H]

Number of amino acids: Translated: 684; Mature: 684

Protein sequence:

>684_residues
MELKATSFGKHLSQHPYNRVRLLNAGIEVCGDKHQYLIPFNQLVSIQCKRGIVWGELEFELPQQKVVRLHGTEWQETQHF
YHYLLGVWQQWSEEMSQVCAEVLHKQVESIKRIEQQDKWFKRSELGQIQVMIREAFSALPMPVERLAAFESCRADYECCL
RWLQQGRHSVERCNQQWTARMLEEYQDFFQNVETSPLNASQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRNEAL
PEQILLLAFGRQAADEMNSRIKQRLGVDSIQAKTFHALALHIIQQGSRKTPVISKLESDSQARRALLIQHWQQQCSEKKA
QAKGWREWLTDELEWDVADGDFWQDKRLAARLASRLERWVGLMRMYGGSQAEMIEQANDEVRDLFQKRIRLMAPLLKAWK
TALKDEGAVDFSGLIHQAVNLLEKGRFVSPWKHVLVDEFQDISPQRAMLLAALRKQNKQTCLFAVGDDWQAIYRFSGAEL
SLTTAFSEHFGEGAECALDTTYRFNDRIGDIANRFIQQNPYQLKKPLNSLSKGNKKSVMILPDEQLEALLDKLSGFVKDG
ERILILARYHHLRPEVLQKAATRWPKLTIDFMTIHASKGQQADYVIIVGLHEGNDGFPAAARESVLEDVLLPPPEDFPDA
EERRLLYVAITRAKHQVWLLQDTARPSIFVSQLSELGALVQRKP

Sequences:

>Translated_684_residues
MELKATSFGKHLSQHPYNRVRLLNAGIEVCGDKHQYLIPFNQLVSIQCKRGIVWGELEFELPQQKVVRLHGTEWQETQHF
YHYLLGVWQQWSEEMSQVCAEVLHKQVESIKRIEQQDKWFKRSELGQIQVMIREAFSALPMPVERLAAFESCRADYECCL
RWLQQGRHSVERCNQQWTARMLEEYQDFFQNVETSPLNASQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRNEAL
PEQILLLAFGRQAADEMNSRIKQRLGVDSIQAKTFHALALHIIQQGSRKTPVISKLESDSQARRALLIQHWQQQCSEKKA
QAKGWREWLTDELEWDVADGDFWQDKRLAARLASRLERWVGLMRMYGGSQAEMIEQANDEVRDLFQKRIRLMAPLLKAWK
TALKDEGAVDFSGLIHQAVNLLEKGRFVSPWKHVLVDEFQDISPQRAMLLAALRKQNKQTCLFAVGDDWQAIYRFSGAEL
SLTTAFSEHFGEGAECALDTTYRFNDRIGDIANRFIQQNPYQLKKPLNSLSKGNKKSVMILPDEQLEALLDKLSGFVKDG
ERILILARYHHLRPEVLQKAATRWPKLTIDFMTIHASKGQQADYVIIVGLHEGNDGFPAAARESVLEDVLLPPPEDFPDA
EERRLLYVAITRAKHQVWLLQDTARPSIFVSQLSELGALVQRKP
>Mature_684_residues
MELKATSFGKHLSQHPYNRVRLLNAGIEVCGDKHQYLIPFNQLVSIQCKRGIVWGELEFELPQQKVVRLHGTEWQETQHF
YHYLLGVWQQWSEEMSQVCAEVLHKQVESIKRIEQQDKWFKRSELGQIQVMIREAFSALPMPVERLAAFESCRADYECCL
RWLQQGRHSVERCNQQWTARMLEEYQDFFQNVETSPLNASQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRNEAL
PEQILLLAFGRQAADEMNSRIKQRLGVDSIQAKTFHALALHIIQQGSRKTPVISKLESDSQARRALLIQHWQQQCSEKKA
QAKGWREWLTDELEWDVADGDFWQDKRLAARLASRLERWVGLMRMYGGSQAEMIEQANDEVRDLFQKRIRLMAPLLKAWK
TALKDEGAVDFSGLIHQAVNLLEKGRFVSPWKHVLVDEFQDISPQRAMLLAALRKQNKQTCLFAVGDDWQAIYRFSGAEL
SLTTAFSEHFGEGAECALDTTYRFNDRIGDIANRFIQQNPYQLKKPLNSLSKGNKKSVMILPDEQLEALLDKLSGFVKDG
ERILILARYHHLRPEVLQKAATRWPKLTIDFMTIHASKGQQADYVIIVGLHEGNDGFPAAARESVLEDVLLPPPEDFPDA
EERRLLYVAITRAKHQVWLLQDTARPSIFVSQLSELGALVQRKP

Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787196, Length=684, Percent_Identity=68.7134502923977, Blast_Score=989, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=338, Percent_Identity=28.4023668639053, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000212
- InterPro:   IPR014016
- InterPro:   IPR022161
- InterPro:   IPR003661 [H]

Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78455; Mature: 78455

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKATSFGKHLSQHPYNRVRLLNAGIEVCGDKHQYLIPFNQLVSIQCKRGIVWGELEFE
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCEEEEEEEEC
LPQQKVVRLHGTEWQETQHFYHYLLGVWQQWSEEMSQVCAEVLHKQVESIKRIEQQDKWF
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRSELGQIQVMIREAFSALPMPVERLAAFESCRADYECCLRWLQQGRHSVERCNQQWTAR
HHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MLEEYQDFFQNVETSPLNASQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRNEAL
HHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCEEEEEHHCHHHHCCCCC
PEQILLLAFGRQAADEMNSRIKQRLGVDSIQAKTFHALALHIIQQGSRKTPVISKLESDS
HHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHH
QARRALLIQHWQQQCSEKKAQAKGWREWLTDELEWDVADGDFWQDKRLAARLASRLERWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCEECCCCCCCHHHHHHHHHHHHHHHHH
GLMRMYGGSQAEMIEQANDEVRDLFQKRIRLMAPLLKAWKTALKDEGAVDFSGLIHQAVN
HHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LLEKGRFVSPWKHVLVDEFQDISPQRAMLLAALRKQNKQTCLFAVGDDWQAIYRFSGAEL
HHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCE
SLTTAFSEHFGEGAECALDTTYRFNDRIGDIANRFIQQNPYQLKKPLNSLSKGNKKSVMI
EEEHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEE
LPDEQLEALLDKLSGFVKDGERILILARYHHLRPEVLQKAATRWPKLTIDFMTIHASKGQ
ECHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCHHHHHHHHHCCCCEEEEEEEEECCCCC
QADYVIIVGLHEGNDGFPAAARESVLEDVLLPPPEDFPDAEERRLLYVAITRAKHQVWLL
CCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCEEEEEEEECCCCEEEEE
QDTARPSIFVSQLSELGALVQRKP
ECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MELKATSFGKHLSQHPYNRVRLLNAGIEVCGDKHQYLIPFNQLVSIQCKRGIVWGELEFE
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCEEEEEEEEC
LPQQKVVRLHGTEWQETQHFYHYLLGVWQQWSEEMSQVCAEVLHKQVESIKRIEQQDKWF
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRSELGQIQVMIREAFSALPMPVERLAAFESCRADYECCLRWLQQGRHSVERCNQQWTAR
HHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MLEEYQDFFQNVETSPLNASQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRNEAL
HHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCEEEEEHHCHHHHCCCCC
PEQILLLAFGRQAADEMNSRIKQRLGVDSIQAKTFHALALHIIQQGSRKTPVISKLESDS
HHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHH
QARRALLIQHWQQQCSEKKAQAKGWREWLTDELEWDVADGDFWQDKRLAARLASRLERWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCEECCCCCCCHHHHHHHHHHHHHHHHH
GLMRMYGGSQAEMIEQANDEVRDLFQKRIRLMAPLLKAWKTALKDEGAVDFSGLIHQAVN
HHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LLEKGRFVSPWKHVLVDEFQDISPQRAMLLAALRKQNKQTCLFAVGDDWQAIYRFSGAEL
HHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCE
SLTTAFSEHFGEGAECALDTTYRFNDRIGDIANRFIQQNPYQLKKPLNSLSKGNKKSVMI
EEEHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEE
LPDEQLEALLDKLSGFVKDGERILILARYHHLRPEVLQKAATRWPKLTIDFMTIHASKGQ
ECHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCHHHHHHHHHCCCCEEEEEEEEECCCCC
QADYVIIVGLHEGNDGFPAAARESVLEDVLLPPPEDFPDAEERRLLYVAITRAKHQVWLL
CCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCEEEEEEEECCCCEEEEE
QDTARPSIFVSQLSELGALVQRKP
ECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2542273; 8905232; 9278503 [H]