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The map label for this gene is hisF [H]

Identifier: 22126507

GI number: 22126507

Start: 2890313

End: 2891089

Strand: Direct

Name: hisF [H]

Synonym: y2627

Alternate gene names: 22126507

Gene position: 2890313-2891089 (Clockwise)

Preceding gene: 22126506

Following gene: 22126508

Centisome position: 62.82

GC content: 50.71

Gene sequence:

>777_bases
ATGCTGGCAAAACGTATAATTCCTTGTCTGGATGTGAAAGACGGCCAGGTCGTTAAAGGTGTTCAGTTCCGCAATCATGA
GATCATCGGCGATATCGTGCCTTTGGCGCAGCGCTATGCTCAAGAAGGGGCTGATGAGTTGGTGTTTTACGATATCACTG
CCTCATCCGATGGCCGTGTCGTCGATAAAAGCTGGGTAGCGCGGGTCGCAGAAGTGATCGATATCCCTTTCTGTGTGGCC
GGGGGGATCAAAAGTGTCGAAGATGCAAGCCAGATCCTGACATTCGGTGCGGATAAAATCTCAATCAACTCTCCCGCATT
GGCAGACCCAACACTCATTACCCGCTTGGCTGACCGCTATGGCGTACAATGCATCGTGGTGGGAATTGATACCTGGTATG
ACACCGAAAGCGACAGCTATCAGGTTTACCAGTTTACCGGTGATGAGAAGCGTACCAAAGCCACCACGTGGCAAACCGAA
GATTGGGTCAAAGAGGTCCAGTTACGGGGAGCCGGTGAGATTGTATTAAATATGATGAATCAGGACGGCGTCCGTAACGG
CTATGATTTGCGTCAGCTTCAACAGATGCGTGCTATTTGCCACGTTCCGTTAATCGCCTCTGGCGGTGCCGGGACACCGG
ATCACTTCCTTGAAGCCTTCCGCGACGCGGATGTTGATGGCGCGCTGGCGGCATCGGTATTCCATAAACAAATCATTAAT
ATCGGCGAATTGAAAAAGTATTTGTCTGAACAGGGCGTGGAGATAAGAGTGTGTTAA

Upstream 100 bases:

>100_bases
GTCTGGATGATATCGCCCGCTTACGCGGCAGCGGTGTACAGGGCGTGATTGTTGGCCGTGCGTTACTGGACGGCAAGTTT
AACGTGAAGGAGGCTATCGC

Downstream 100 bases:

>100_bases
CAGAACAACAAATCAACCAATTGGATTGGGAAAAAGTCGATCACCTGATGCCAGCCATCGTTCAGCATGCCGTATCCGGC
GAAGTTCTGATGATGGGGTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKSVEDASQILTFGADKISINSPALADPTLITRLADRYGVQCIVVGIDTWYDTESDSYQVYQFTGDEKRTKATTWQTE
DWVKEVQLRGAGEIVLNMMNQDGVRNGYDLRQLQQMRAICHVPLIASGGAGTPDHFLEAFRDADVDGALAASVFHKQIIN
IGELKKYLSEQGVEIRVC

Sequences:

>Translated_258_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKSVEDASQILTFGADKISINSPALADPTLITRLADRYGVQCIVVGIDTWYDTESDSYQVYQFTGDEKRTKATTWQTE
DWVKEVQLRGAGEIVLNMMNQDGVRNGYDLRQLQQMRAICHVPLIASGGAGTPDHFLEAFRDADVDGALAASVFHKQIIN
IGELKKYLSEQGVEIRVC
>Mature_258_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKSVEDASQILTFGADKISINSPALADPTLITRLADRYGVQCIVVGIDTWYDTESDSYQVYQFTGDEKRTKATTWQTE
DWVKEVQLRGAGEIVLNMMNQDGVRNGYDLRQLQQMRAICHVPLIASGGAGTPDHFLEAFRDADVDGALAASVFHKQIIN
IGELKKYLSEQGVEIRVC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=86.046511627907, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI87082028, Length=234, Percent_Identity=24.7863247863248, Blast_Score=67, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6319725, Length=317, Percent_Identity=30.9148264984227, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28511; Mature: 28511

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIKSVEDASQILTFGADKISINSPALADPTLITRLADRY
ECHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWYDTESDSYQVYQFTGDEKRTKATTWQTEDWVKEVQLRGAGEIVLNMMN
CCEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
QDGVRNGYDLRQLQQMRAICHVPLIASGGAGTPDHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHC
IGELKKYLSEQGVEIRVC
HHHHHHHHHHCCCEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIKSVEDASQILTFGADKISINSPALADPTLITRLADRY
ECHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWYDTESDSYQVYQFTGDEKRTKATTWQTEDWVKEVQLRGAGEIVLNMMN
CCEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
QDGVRNGYDLRQLQQMRAICHVPLIASGGAGTPDHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHC
IGELKKYLSEQGVEIRVC
HHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA