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The map label for this gene is lacY [H]

Identifier: 22126462

GI number: 22126462

Start: 2842618

End: 2843862

Strand: Direct

Name: lacY [H]

Synonym: y2581

Alternate gene names: 22126462

Gene position: 2842618-2843862 (Clockwise)

Preceding gene: 22126457

Following gene: 22126463

Centisome position: 61.79

GC content: 44.5

Gene sequence:

>1245_bases
TTGTTTACAGTCTCCTTTTTTATCATGGCATCGTACTACCCGTTCTTTCCGATCTGGCTGCATGATATTAATAACCTGAG
CAAAACAGACACCGGTATTGTATTCGGTAGCATTTCACTTTTTGCCCTCGCCTTTCAGCCAATTATGGGGCCGCTCTCTG
ACAAGCTTGGATTACGCAAAACCCTAATGTGGATCATTGTTGGATTACTGGTCCTGTTTGCCCCGTTCTTTATTTATGTT
TTCAGCCCATTACTGAAATATAACATCTTCATTGGCGCTATTGTGGGTGGCTGCTATTTGGGCTTTGTATTTACTGGCGG
TTCCCATGCCATTGAAGCCTATATTGAAAAGGTCAGTCGCCACAGTAATTTTGAATACGGCCGGGTAAGAATGTTCGGCT
GTATTGGCTGGGCACTGTGCGCCACCGTGGTTGGAATACTCTACACCGTCAACAACCAATTAATTTTTTGGATGGCATCA
GGCTGCGCCTTAATACTGGCTGTCTTGCTGTTCTTCGCTCGCCCCGACCGACAATCTACGGCCTTTGTTGTCGATACTCT
AGGCGCGAATAAAGCGGTATTTAACTTAAAGAACGCGCTGGCACTCCTCCGGAAACGGGAGTTGTGGTTTTTCGTTATGT
ATATTGTTGGCGTCGCCTGTATTTATGATGTGTTTGATCAGCAATTTGCTAACTTTTTCACTTCATTCTTTGCCACCAAA
CAACAAGGAACTGAAATATTCGGTTTTGTCACTACCGGTGGTGAAATTCTGAATGCAACCGTGATGTTTTTCGCTCCGGT
AATTATTGCGCGTATTGGTAGCAAAAATGCGTTATTGCTGGCAGGTACCATTATGTCAGTCCGTATTTTAGGTTCAGCAT
TTGCCACGACAGCGACCCAAGTGGTGTTCCTGAAAATGTTGCATATGTTTGAGGTTCCTTTCTTACTGGTAGGCTCCTTT
AAATATATTACTCAAGTATTTGAAGTTCGTTTTTCAGCAACGGTGTATCTGATTGGGTTCTGTTTCAGCAAACAGTTATC
CATGATGTTTATGTCAGTATTTGCTGGCCGCATGTATGACAGCATGGGATATCAAGACACGTACATGGTATTAGGCGTTA
TCGTCCTGTCATTTACCCTCATTTCAGCTTTCACGCTCTCTGGGCGCAGCGCGGTAGCAAACCTGGCGTCAAGGCTAAAA
GAGGATCCGTCGGTTACCCCTCCAGTTTCTCAGCCACAATCTTAA

Upstream 100 bases:

>100_bases
TAGCCAGTTCAGCCGCAAGTGCTTTAAGTTTCTTTTCGTCCATAATTTGCCTGTCTCCGTTGTTGGAGTGAAGATATCAA
AAACAGGCAATTACACAATA

Downstream 100 bases:

>100_bases
AAAATTCAATACAGATAACGAGGGTGTCGCCGCCATGCCATCACCTCGAGCAAATTTTCGTTTTCACAGGAGCCATTATG
AATACTACCTTTGTCAGATT

Product: galactoside permease

Products: Proton [Cytoplasm]; lactose [Cytoplasm] [C]

Alternate protein names: Lactose-proton symport [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MFTVSFFIMASYYPFFPIWLHDINNLSKTDTGIVFGSISLFALAFQPIMGPLSDKLGLRKTLMWIIVGLLVLFAPFFIYV
FSPLLKYNIFIGAIVGGCYLGFVFTGGSHAIEAYIEKVSRHSNFEYGRVRMFGCIGWALCATVVGILYTVNNQLIFWMAS
GCALILAVLLFFARPDRQSTAFVVDTLGANKAVFNLKNALALLRKRELWFFVMYIVGVACIYDVFDQQFANFFTSFFATK
QQGTEIFGFVTTGGEILNATVMFFAPVIIARIGSKNALLLAGTIMSVRILGSAFATTATQVVFLKMLHMFEVPFLLVGSF
KYITQVFEVRFSATVYLIGFCFSKQLSMMFMSVFAGRMYDSMGYQDTYMVLGVIVLSFTLISAFTLSGRSAVANLASRLK
EDPSVTPPVSQPQS

Sequences:

>Translated_414_residues
MFTVSFFIMASYYPFFPIWLHDINNLSKTDTGIVFGSISLFALAFQPIMGPLSDKLGLRKTLMWIIVGLLVLFAPFFIYV
FSPLLKYNIFIGAIVGGCYLGFVFTGGSHAIEAYIEKVSRHSNFEYGRVRMFGCIGWALCATVVGILYTVNNQLIFWMAS
GCALILAVLLFFARPDRQSTAFVVDTLGANKAVFNLKNALALLRKRELWFFVMYIVGVACIYDVFDQQFANFFTSFFATK
QQGTEIFGFVTTGGEILNATVMFFAPVIIARIGSKNALLLAGTIMSVRILGSAFATTATQVVFLKMLHMFEVPFLLVGSF
KYITQVFEVRFSATVYLIGFCFSKQLSMMFMSVFAGRMYDSMGYQDTYMVLGVIVLSFTLISAFTLSGRSAVANLASRLK
EDPSVTPPVSQPQS
>Mature_414_residues
MFTVSFFIMASYYPFFPIWLHDINNLSKTDTGIVFGSISLFALAFQPIMGPLSDKLGLRKTLMWIIVGLLVLFAPFFIYV
FSPLLKYNIFIGAIVGGCYLGFVFTGGSHAIEAYIEKVSRHSNFEYGRVRMFGCIGWALCATVVGILYTVNNQLIFWMAS
GCALILAVLLFFARPDRQSTAFVVDTLGANKAVFNLKNALALLRKRELWFFVMYIVGVACIYDVFDQQFANFFTSFFATK
QQGTEIFGFVTTGGEILNATVMFFAPVIIARIGSKNALLLAGTIMSVRILGSAFATTATQVVFLKMLHMFEVPFLLVGSF
KYITQVFEVRFSATVYLIGFCFSKQLSMMFMSVFAGRMYDSMGYQDTYMVLGVIVLSFTLISAFTLSGRSAVANLASRLK
EDPSVTPPVSQPQS

Specific function: Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family [H]

Homologues:

Organism=Escherichia coli, GI1786538, Length=394, Percent_Identity=68.0203045685279, Blast_Score=573, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR000576
- InterPro:   IPR018457
- InterPro:   IPR020846
- InterPro:   IPR016196 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 46068; Mature: 46068

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTVSFFIMASYYPFFPIWLHDINNLSKTDTGIVFGSISLFALAFQPIMGPLSDKLGLRK
CEEEHEEHHHHHCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCHHHHHHHHHH
TLMWIIVGLLVLFAPFFIYVFSPLLKYNIFIGAIVGGCYLGFVFTGGSHAIEAYIEKVSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHH
HSNFEYGRVRMFGCIGWALCATVVGILYTVNNQLIFWMASGCALILAVLLFFARPDRQST
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCCC
AFVVDTLGANKAVFNLKNALALLRKRELWFFVMYIVGVACIYDVFDQQFANFFTSFFATK
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQGTEIFGFVTTGGEILNATVMFFAPVIIARIGSKNALLLAGTIMSVRILGSAFATTATQ
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
VVFLKMLHMFEVPFLLVGSFKYITQVFEVRFSATVYLIGFCFSKQLSMMFMSVFAGRMYD
HHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMGYQDTYMVLGVIVLSFTLISAFTLSGRSAVANLASRLKEDPSVTPPVSQPQS
HCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFTVSFFIMASYYPFFPIWLHDINNLSKTDTGIVFGSISLFALAFQPIMGPLSDKLGLRK
CEEEHEEHHHHHCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCHHHHHHHHHH
TLMWIIVGLLVLFAPFFIYVFSPLLKYNIFIGAIVGGCYLGFVFTGGSHAIEAYIEKVSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHH
HSNFEYGRVRMFGCIGWALCATVVGILYTVNNQLIFWMASGCALILAVLLFFARPDRQST
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCCC
AFVVDTLGANKAVFNLKNALALLRKRELWFFVMYIVGVACIYDVFDQQFANFFTSFFATK
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQGTEIFGFVTTGGEILNATVMFFAPVIIARIGSKNALLLAGTIMSVRILGSAFATTATQ
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
VVFLKMLHMFEVPFLLVGSFKYITQVFEVRFSATVYLIGFCFSKQLSMMFMSVFAGRMYD
HHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMGYQDTYMVLGVIVLSFTLISAFTLSGRSAVANLASRLKEDPSVTPPVSQPQS
HCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; lactose [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactose [Periplasm] = Proton [Cytoplasm] + lactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6444453; 9278503; 2164211; 7578103; 1848449; 1644770; 2203471; 10485888; 12893935 [H]