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The map label for this gene is ruvC [H]

Identifier: 22126140

GI number: 22126140

Start: 2484094

End: 2484615

Strand: Reverse

Name: ruvC [H]

Synonym: y2254

Alternate gene names: 22126140

Gene position: 2484615-2484094 (Counterclockwise)

Preceding gene: 161484794

Following gene: 161484795

Centisome position: 54.0

GC content: 50.77

Gene sequence:

>522_bases
ATGGCGATCGTATTAGGTATCGACCCCGGTTCTCGTGTTACCGGCTATGGTGTTATCCGCCAGCAAGGTCGTCAACTTAC
GTATCTGGGCAGTGGCTGTATCCGCACTGTGGTTGACGATATGCCTACTCGCTTGAAGTTGATATACGCAGGTGTGACCG
AAATCATTACGCAGTTTCAGCCAGATTTTTTTGCTATCGAACAAGTCTTTATGGCAAAAAATCCGGACTCGGCATTAAAA
CTGGGGCAAGCGCGTGGCGCGGCCATTGTGGCGGCAGTAAACCTCAATTTACCTGTCTCTGAATACGCCGCACGTCAGGT
AAAACAGACGGTTGTAGGGACCGGCGCGGCAGAAAAAAGCCAGGTCCAGCACATGGTGCGCTCCCTGCTTAAATTACCGG
CGAATCCACAAGCGGATGCGGCGGATGCATTAGCTATCGCCATTACACATTGTCATCTGAGCCAAAATACGTTGCGTTTG
GGTAATGACCAAATGACATTATCACGTGGCCGGATACGCTGA

Upstream 100 bases:

>100_bases
ACGCACAGGCAACTTGAAGTAGGACGGGGATATCGGGTGAGGTGCTTTCCCCATCAATACGGCTGCTCAATCAGGTGGCG
AATAAGGTAAATAAACGAGT

Downstream 100 bases:

>100_bases
CCGATCAGGTTTATGCGAACAGGTTAACTGTTATCACAGGCTGGATATCCATCCAGCCTTTTTTATGCTATAAACAGTGC
TGTTTGAGTGATAAACACAA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MAIVLGIDPGSRVTGYGVIRQQGRQLTYLGSGCIRTVVDDMPTRLKLIYAGVTEIITQFQPDFFAIEQVFMAKNPDSALK
LGQARGAAIVAAVNLNLPVSEYAARQVKQTVVGTGAAEKSQVQHMVRSLLKLPANPQADAADALAIAITHCHLSQNTLRL
GNDQMTLSRGRIR

Sequences:

>Translated_173_residues
MAIVLGIDPGSRVTGYGVIRQQGRQLTYLGSGCIRTVVDDMPTRLKLIYAGVTEIITQFQPDFFAIEQVFMAKNPDSALK
LGQARGAAIVAAVNLNLPVSEYAARQVKQTVVGTGAAEKSQVQHMVRSLLKLPANPQADAADALAIAITHCHLSQNTLRL
GNDQMTLSRGRIR
>Mature_172_residues
AIVLGIDPGSRVTGYGVIRQQGRQLTYLGSGCIRTVVDDMPTRLKLIYAGVTEIITQFQPDFFAIEQVFMAKNPDSALKL
GQARGAAIVAAVNLNLPVSEYAARQVKQTVVGTGAAEKSQVQHMVRSLLKLPANPQADAADALAIAITHCHLSQNTLRLG
NDQMTLSRGRIR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=173, Percent_Identity=83.8150289017341, Blast_Score=281, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 18641; Mature: 18510

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVLGIDPGSRVTGYGVIRQQGRQLTYLGSGCIRTVVDDMPTRLKLIYAGVTEIITQFQ
CEEEEECCCCCCEEHHHHHHHCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
PDFFAIEQVFMAKNPDSALKLGQARGAAIVAAVNLNLPVSEYAARQVKQTVVGTGAAEKS
CCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHH
QVQHMVRSLLKLPANPQADAADALAIAITHCHLSQNTLRLGNDQMTLSRGRIR
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCEECCCCCCC
>Mature Secondary Structure 
AIVLGIDPGSRVTGYGVIRQQGRQLTYLGSGCIRTVVDDMPTRLKLIYAGVTEIITQFQ
EEEEECCCCCCEEHHHHHHHCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
PDFFAIEQVFMAKNPDSALKLGQARGAAIVAAVNLNLPVSEYAARQVKQTVVGTGAAEKS
CCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHH
QVQHMVRSLLKLPANPQADAADALAIAITHCHLSQNTLRLGNDQMTLSRGRIR
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA