Click here to switch to the map view.

The map label for this gene is purU [H]

Identifier: 22126038

GI number: 22126038

Start: 2377153

End: 2377905

Strand: Direct

Name: purU [H]

Synonym: y2150

Alternate gene names: 22126038

Gene position: 2377153-2377905 (Clockwise)

Preceding gene: 22126037

Following gene: 161484800

Centisome position: 51.67

GC content: 44.36

Gene sequence:

>753_bases
ATGAATTTTGTTGATCACCTGACTGGTCGCTTCTTTATGCGCACCGAGCTTGAGGGGATTTTTAACGATACGACATTACT
GGCAGATCTTGACGATGCATTACCTGAAGGCACTAATCGCGAGTTACACGTTGCTGGGCGCCGCCGCATCATCATTATGG
TAACGAAAGAAGCACATTGCCTTGGCGACTTATTAATGAAGAGTGCTTATGGTGGCTTAGATGTAGAAATTGCGGCAGTC
ATTGGTAACCATGATGCATTGCAAAATTTAGTCGAGCGTTTTGATATCCCATTCCATCTGGTCAGCCATGAAGGCCTCAC
CCGTGAGCAGCATGATCAACAGTTGATTGAGAAAATTGAACAATATCAACCAGATTATGTTGTTCTGGCTAAATATATGC
GGGTGCTAACACCGGCCTTTGTGCAACGTTTCCCTTATCAAATTATTAATATTCATCATTCATTTTTGCCTGCATTTATC
GGTGCTCGGCCATACCACCAAGCGTATGAACGCGGAGTGAAGATTATTGGCGCTACTGCTCATTACGTAAATGATAGCTT
GGATGAAGGCCCAATCATCATGCAGGACGTGATCAATGTGGATCATTCTTATACTGCTGAAGATATGATGCGTGCTGGTC
GTGATGTAGAAAAGAATGTTCTCAGTAGTGCGCTATACCGGAGCCTGTCCATAATTCTGTGTAACTGCCACCGTATTAAA
GGTGATCGCTCAGGCGGTCACCGAACTCGATAA

Upstream 100 bases:

>100_bases
AAAAGAAAGTATTGCGCACTATTTGCCCTGACGCTAAAAGGTTTGATCGCAAAGATCACTAATATCTGCTACAAGCACCA
ATTGAATATCGTACAAAATA

Downstream 100 bases:

>100_bases
TAAAACGGCTCATCGCTAACCGCCAGTTCTGGATCGGCATACTCCATTTTTTTGATGCATCCTTGATCGCCAAATAAACA
ACCTTCCGCACTGAGTCGTC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MNFVDHLTGRFFMRTELEGIFNDTTLLADLDDALPEGTNRELHVAGRRRIIIMVTKEAHCLGDLLMKSAYGGLDVEIAAV
IGNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIEQYQPDYVVLAKYMRVLTPAFVQRFPYQIINIHHSFLPAFI
GARPYHQAYERGVKIIGATAHYVNDSLDEGPIIMQDVINVDHSYTAEDMMRAGRDVEKNVLSSALYRSLSIILCNCHRIK
GDRSGGHRTR

Sequences:

>Translated_250_residues
MNFVDHLTGRFFMRTELEGIFNDTTLLADLDDALPEGTNRELHVAGRRRIIIMVTKEAHCLGDLLMKSAYGGLDVEIAAV
IGNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIEQYQPDYVVLAKYMRVLTPAFVQRFPYQIINIHHSFLPAFI
GARPYHQAYERGVKIIGATAHYVNDSLDEGPIIMQDVINVDHSYTAEDMMRAGRDVEKNVLSSALYRSLSIILCNCHRIK
GDRSGGHRTR
>Mature_250_residues
MNFVDHLTGRFFMRTELEGIFNDTTLLADLDDALPEGTNRELHVAGRRRIIIMVTKEAHCLGDLLMKSAYGGLDVEIAAV
IGNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIEQYQPDYVVLAKYMRVLTPAFVQRFPYQIINIHHSFLPAFI
GARPYHQAYERGVKIIGATAHYVNDSLDEGPIIMQDVINVDHSYTAEDMMRAGRDVEKNVLSSALYRSLSIILCNCHRIK
GDRSGGHRTR

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=182, Percent_Identity=29.6703296703297, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869995, Length=182, Percent_Identity=29.6703296703297, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869993, Length=182, Percent_Identity=29.6703296703297, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787483, Length=228, Percent_Identity=84.6491228070175, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1788846, Length=94, Percent_Identity=44.6808510638298, Blast_Score=91, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=163, Percent_Identity=33.1288343558282, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24582400, Length=159, Percent_Identity=29.559748427673, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 28483; Mature: 28483

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFVDHLTGRFFMRTELEGIFNDTTLLADLDDALPEGTNRELHVAGRRRIIIMVTKEAHC
CCHHHHHHHHHHHHHHHHHHHCCCHHEEECHHHCCCCCCCEEEECCCCEEEEEEECCHHH
LGDLLMKSAYGGLDVEIAAVIGNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIE
HHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHH
QYQPDYVVLAKYMRVLTPAFVQRFPYQIINIHHSFLPAFIGARPYHQAYERGVKIIGATA
HCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHHH
HYVNDSLDEGPIIMQDVINVDHSYTAEDMMRAGRDVEKNVLSSALYRSLSIILCNCHRIK
HHHCCCCCCCCCHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSGGHRTR
CCCCCCCCCC
>Mature Secondary Structure
MNFVDHLTGRFFMRTELEGIFNDTTLLADLDDALPEGTNRELHVAGRRRIIIMVTKEAHC
CCHHHHHHHHHHHHHHHHHHHCCCHHEEECHHHCCCCCCCEEEECCCCEEEEEEECCHHH
LGDLLMKSAYGGLDVEIAAVIGNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIE
HHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHH
QYQPDYVVLAKYMRVLTPAFVQRFPYQIINIHHSFLPAFIGARPYHQAYERGVKIIGATA
HCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHHH
HYVNDSLDEGPIIMQDVINVDHSYTAEDMMRAGRDVEKNVLSSALYRSLSIILCNCHRIK
HHHCCCCCCCCCHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSGGHRTR
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]