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The map label for this gene is rstA [H]

Identifier: 22126027

GI number: 22126027

Start: 2363830

End: 2364630

Strand: Direct

Name: rstA [H]

Synonym: y2139

Alternate gene names: 22126027

Gene position: 2363830-2364630 (Clockwise)

Preceding gene: 22126025

Following gene: 22126028

Centisome position: 51.38

GC content: 47.57

Gene sequence:

>801_bases
TTGTCATTAACTGCACGCTATTCTGAGCCCAGCTATTTTTATAAGCAGGAAGACATGAGCAAAATTGTTTTTGTGGAAGA
CGACCCAGAGGTCGGAAAGCTGATAGCGGCTTACCTTGGCAAGCATGACATTGATGTATTTGTTGAGCCACGTGGTGATA
CCGCACAAGCCGTTATTGAACAGCAACAGCCCGATCTGGTGTTGCTCGATATCATGTTACCTGGGAAAGATGGCATGACC
CTGTGCCGTGACCTACGGCCCCACTATGATGGCCCAATAGTGTTGCTGACATCGCTGGACAGCGATATGAACCATATTCT
CTCCCTAGAGATGGGGGCAAATGACTATATTCTAAAAACGACACCGCCAGCCGTGCTGCTCGCCCGCTTACGGCTACATT
TGCGCCAACATAATCAACGGTTACGCCAACAGACTCCCTTGCAAGCCAAAGAGACAATAAGCTCCCCACCCACGGGTTAC
AATGCGATTCATTTTGGGCTGCTATGTATTGACCCAGTTAACCGGCAAGTCAATTTGGATGATGAAGAAATTTCCTTATC
AACTTCTGACTTTGATCTTTTATGGGAATTGGCGACACATGCTGGAATCATTATGGCTCGCGAGGCGTTATTGAAAAATT
TACGCGGGATCAGCTATGACGGTATGGACCGAAGTATAGATGTCGCTATCTCTCGCTTACGCCGCAAACTCTATGACAAT
GCACTGGACCCATTCCGGATCAAAACGGTGCGTAACAAAGGTTATTTGTTTGCGCCCAATGCCTGGGAGTCCAAGCAGTA
A

Upstream 100 bases:

>100_bases
TCGACGACATTAATAAACCGTTTAATAAGTAATGGTATTATCGAGTGTCTGCCCTTGTTACGGCCTGTCTTGTTACTGCT
AGTTTTGCTATTAACTAGTC

Downstream 100 bases:

>100_bases
TGAGAAAGCTATTTATTCAGTTTTTTTTGTTGTTATTCGTTTGCTTCATGGTGATGGCGATGCTGGTTGGCCTGGTTTAT
AAGGTCACCGCTGAGCGCGC

Product: DNA-binding transcriptional regulator RstA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSLTARYSEPSYFYKQEDMSKIVFVEDDPEVGKLIAAYLGKHDIDVFVEPRGDTAQAVIEQQQPDLVLLDIMLPGKDGMT
LCRDLRPHYDGPIVLLTSLDSDMNHILSLEMGANDYILKTTPPAVLLARLRLHLRQHNQRLRQQTPLQAKETISSPPTGY
NAIHFGLLCIDPVNRQVNLDDEEISLSTSDFDLLWELATHAGIIMAREALLKNLRGISYDGMDRSIDVAISRLRRKLYDN
ALDPFRIKTVRNKGYLFAPNAWESKQ

Sequences:

>Translated_266_residues
MSLTARYSEPSYFYKQEDMSKIVFVEDDPEVGKLIAAYLGKHDIDVFVEPRGDTAQAVIEQQQPDLVLLDIMLPGKDGMT
LCRDLRPHYDGPIVLLTSLDSDMNHILSLEMGANDYILKTTPPAVLLARLRLHLRQHNQRLRQQTPLQAKETISSPPTGY
NAIHFGLLCIDPVNRQVNLDDEEISLSTSDFDLLWELATHAGIIMAREALLKNLRGISYDGMDRSIDVAISRLRRKLYDN
ALDPFRIKTVRNKGYLFAPNAWESKQ
>Mature_265_residues
SLTARYSEPSYFYKQEDMSKIVFVEDDPEVGKLIAAYLGKHDIDVFVEPRGDTAQAVIEQQQPDLVLLDIMLPGKDGMTL
CRDLRPHYDGPIVLLTSLDSDMNHILSLEMGANDYILKTTPPAVLLARLRLHLRQHNQRLRQQTPLQAKETISSPPTGYN
AIHFGLLCIDPVNRQVNLDDEEISLSTSDFDLLWELATHAGIIMAREALLKNLRGISYDGMDRSIDVAISRLRRKLYDNA
LDPFRIKTVRNKGYLFAPNAWESKQ

Specific function: Member of the two-component regulatory system rstB/rstA [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693140, Length=245, Percent_Identity=73.0612244897959, Blast_Score=338, Evalue=2e-94,
Organism=Escherichia coli, GI1789809, Length=245, Percent_Identity=33.469387755102, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1788394, Length=245, Percent_Identity=34.6938775510204, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI87082012, Length=244, Percent_Identity=33.6065573770492, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI2367329, Length=240, Percent_Identity=31.25, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786599, Length=241, Percent_Identity=29.4605809128631, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787229, Length=241, Percent_Identity=31.1203319502075, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786784, Length=238, Percent_Identity=31.0924369747899, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790863, Length=238, Percent_Identity=29.4117647058824, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786911, Length=239, Percent_Identity=26.3598326359833, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790860, Length=235, Percent_Identity=28.936170212766, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1790552, Length=243, Percent_Identity=30.0411522633745, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1789402, Length=239, Percent_Identity=26.3598326359833, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787375, Length=196, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 30221; Mature: 30090

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTARYSEPSYFYKQEDMSKIVFVEDDPEVGKLIAAYLGKHDIDVFVEPRGDTAQAVIE
CCCCCCCCCCCCEEECCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
QQQPDLVLLDIMLPGKDGMTLCRDLRPHYDGPIVLLTSLDSDMNHILSLEMGANDYILKT
CCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCEEEEEECCCCCHHEEEEEECCCCEEEEC
TPPAVLLARLRLHLRQHNQRLRQQTPLQAKETISSPPTGYNAIHFGLLCIDPVNRQVNLD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCCEECCC
DEEISLSTSDFDLLWELATHAGIIMAREALLKNLRGISYDGMDRSIDVAISRLRRKLYDN
HHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
ALDPFRIKTVRNKGYLFAPNAWESKQ
CCCCEEEEEECCCCEEECCCCCCCCC
>Mature Secondary Structure 
SLTARYSEPSYFYKQEDMSKIVFVEDDPEVGKLIAAYLGKHDIDVFVEPRGDTAQAVIE
CCCCCCCCCCCEEECCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
QQQPDLVLLDIMLPGKDGMTLCRDLRPHYDGPIVLLTSLDSDMNHILSLEMGANDYILKT
CCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCEEEEEECCCCCHHEEEEEECCCCEEEEC
TPPAVLLARLRLHLRQHNQRLRQQTPLQAKETISSPPTGYNAIHFGLLCIDPVNRQVNLD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCCEECCC
DEEISLSTSDFDLLWELATHAGIIMAREALLKNLRGISYDGMDRSIDVAISRLRRKLYDN
HHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
ALDPFRIKTVRNKGYLFAPNAWESKQ
CCCCEEEEEECCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1495392 [H]