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The map label for this gene is tyrS [H]

Identifier: 22125858

GI number: 22125858

Start: 2165848

End: 2167134

Strand: Direct

Name: tyrS [H]

Synonym: y1966

Alternate gene names: 22125858

Gene position: 2165848-2167134 (Clockwise)

Preceding gene: 22125857

Following gene: 22125859

Centisome position: 47.08

GC content: 48.8

Gene sequence:

>1287_bases
ATGGAGTCATTGATGACTAGCAGCAACCTGATTAAACAATTGCAAGAGCGGGGCCTCGTTGCCCAGGTTACGGATGAAGA
CGCGTTAGCAGAGAGACTGGCGCAAGGGCCAATTTCACTGTATTGCGGTTTCGATCCTACCGCAGACAGCTTGCACTTGG
GTCATCTGGTTCCCTTGTTATGTCTGAAGCGTTTTCAGTTAGCGGGTCACCGCCCGGTGGCGTTGGTCGGGGGCGCTACC
GGTATGATTGGCGACCCAAGCTTCAAAGCCAGTGAGCGTAAATTGAATACCGAAGATACAGTGAATGAGTGGGTCGAGAA
AATCCGCCACCAGGTCTCGCCATTCCTGGATTTTGATTGCGGTGAAAACAGTGCTATTGCAGCCAATAACTATGATTGGT
TTGGTGGCATGAATGTGCTCACTTTCCTGCGTGATATTGGCAAACACTTCTCAGTTAATCAGATGATTAATAAAGAAGCC
GTCAAGCAGCGCTTGAACCGTGATGACAGCGGTATCTCGTTTACTGAGTTCTCATACAATTTATTGCAGGCGTATGATTT
CGCTTGTTTAAACAAAAATCACGGTGTTGCGCTGCAAATCGGTGGTTCTGACCAGTGGGGGAATATTACCTCCGGTATTG
ACCTGACCCGTCGCCTCCATCAACAGCAGGTTTATGGCCTGACGGTACCGCTGATCACCAAAGCCGATGGCACTAAATTC
GGTAAAACCGAAGGGGGTGCAGTCTGGCTGGATCCAAAGAAAACCAGTCCTTACAAATTCTACCAATTCTGGATTAATAC
TGCCGATGCTGATGTGTATCGCTTCCTGAAATTCTTTACTTTCATGAGTTTGGAAGAGATTAACGCACTGGAAGAAGAAG
ATAAAAACAGTGGTAAAGCACCGCGCGCGCAATATGTTTTGGCGGAAAATGTCACGGGTATGGTGCATGGTCCAGAAGGC
TTGGCAGCGGCGAAACGTATCACTGACAGCCTGTTTTCGGGTGATTTGCATGATATGACCGAAGCAGACTTTGCCCAGTT
GGCACAAGATGGTATGCCGACGGTTGAGCTGAACCGTGATGCTGATTTACAGCAAGCGCTGGTTAATGCTGAGCTGGTTC
CTTCCCGTGGTCAGGCGCGCACGATGATTGGTTCTAATGCCGTGGCCATCAATGGTGAGAAACAGGCTGATCCAGAATAC
GTCTTTACGGATGCGGATCGCCTGTTTGGCCGTTATACTTTGTTGCGCCGTGGCAAAAAACACTATTGCCTGATTAGTTG
GCTGTAA

Upstream 100 bases:

>100_bases
ATTAAAAGCCTATGCCGATTGAAAATATATGCGCGCCGCTTGTGTGGTGTTTCCGTTTAGCTAACACATACTTTGTAAAA
GCCAATTTTTGTAAAAGACA

Downstream 100 bases:

>100_bases
GCGTAAAATAAGAGAAGGGGCAGAAATGTCCCTTATTTTTTTGCTGAGACCTGACTCTTTTAAGTCAGTGATTCGTGTGA
GCGAAAACGGCTAACACCGC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MESLMTSSNLIKQLQERGLVAQVTDEDALAERLAQGPISLYCGFDPTADSLHLGHLVPLLCLKRFQLAGHRPVALVGGAT
GMIGDPSFKASERKLNTEDTVNEWVEKIRHQVSPFLDFDCGENSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEA
VKQRLNRDDSGISFTEFSYNLLQAYDFACLNKNHGVALQIGGSDQWGNITSGIDLTRRLHQQQVYGLTVPLITKADGTKF
GKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSLEEINALEEEDKNSGKAPRAQYVLAENVTGMVHGPEG
LAAAKRITDSLFSGDLHDMTEADFAQLAQDGMPTVELNRDADLQQALVNAELVPSRGQARTMIGSNAVAINGEKQADPEY
VFTDADRLFGRYTLLRRGKKHYCLISWL

Sequences:

>Translated_428_residues
MESLMTSSNLIKQLQERGLVAQVTDEDALAERLAQGPISLYCGFDPTADSLHLGHLVPLLCLKRFQLAGHRPVALVGGAT
GMIGDPSFKASERKLNTEDTVNEWVEKIRHQVSPFLDFDCGENSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEA
VKQRLNRDDSGISFTEFSYNLLQAYDFACLNKNHGVALQIGGSDQWGNITSGIDLTRRLHQQQVYGLTVPLITKADGTKF
GKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSLEEINALEEEDKNSGKAPRAQYVLAENVTGMVHGPEG
LAAAKRITDSLFSGDLHDMTEADFAQLAQDGMPTVELNRDADLQQALVNAELVPSRGQARTMIGSNAVAINGEKQADPEY
VFTDADRLFGRYTLLRRGKKHYCLISWL
>Mature_428_residues
MESLMTSSNLIKQLQERGLVAQVTDEDALAERLAQGPISLYCGFDPTADSLHLGHLVPLLCLKRFQLAGHRPVALVGGAT
GMIGDPSFKASERKLNTEDTVNEWVEKIRHQVSPFLDFDCGENSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEA
VKQRLNRDDSGISFTEFSYNLLQAYDFACLNKNHGVALQIGGSDQWGNITSGIDLTRRLHQQQVYGLTVPLITKADGTKF
GKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSLEEINALEEEDKNSGKAPRAQYVLAENVTGMVHGPEG
LAAAKRITDSLFSGDLHDMTEADFAQLAQDGMPTVELNRDADLQQALVNAELVPSRGQARTMIGSNAVAINGEKQADPEY
VFTDADRLFGRYTLLRRGKKHYCLISWL

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=408, Percent_Identity=37.0098039215686, Blast_Score=244, Evalue=1e-64,
Organism=Escherichia coli, GI1787925, Length=423, Percent_Identity=86.2884160756501, Blast_Score=775, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25145802, Length=409, Percent_Identity=35.6968215158924, Blast_Score=228, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI25145805, Length=313, Percent_Identity=33.8658146964856, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6325160, Length=438, Percent_Identity=35.8447488584475, Blast_Score=269, Evalue=7e-73,
Organism=Drosophila melanogaster, GI20130383, Length=446, Percent_Identity=36.5470852017937, Blast_Score=292, Evalue=3e-79,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47619; Mature: 47619

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLMTSSNLIKQLQERGLVAQVTDEDALAERLAQGPISLYCGFDPTADSLHLGHLVPLL
CCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCEEHHHHHHHH
CLKRFQLAGHRPVALVGGATGMIGDPSFKASERKLNTEDTVNEWVEKIRHQVSPFLDFDC
HHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCEECCC
GENSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDSGISFTEFSYN
CCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
LLQAYDFACLNKNHGVALQIGGSDQWGNITSGIDLTRRLHQQQVYGLTVPLITKADGTKF
HHHHHHHHEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCC
GKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSLEEINALEEEDKNSGKA
CCCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRAQYVLAENVTGMVHGPEGLAAAKRITDSLFSGDLHDMTEADFAQLAQDGMPTVELNRD
CCCEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCC
ADLQQALVNAELVPSRGQARTMIGSNAVAINGEKQADPEYVFTDADRLFGRYTLLRRGKK
CHHHHHHHCCCCCCCCCCCEEEECCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHCCCC
HYCLISWL
CEEEEEEC
>Mature Secondary Structure
MESLMTSSNLIKQLQERGLVAQVTDEDALAERLAQGPISLYCGFDPTADSLHLGHLVPLL
CCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCEEHHHHHHHH
CLKRFQLAGHRPVALVGGATGMIGDPSFKASERKLNTEDTVNEWVEKIRHQVSPFLDFDC
HHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCEECCC
GENSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDSGISFTEFSYN
CCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
LLQAYDFACLNKNHGVALQIGGSDQWGNITSGIDLTRRLHQQQVYGLTVPLITKADGTKF
HHHHHHHHEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCC
GKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSLEEINALEEEDKNSGKA
CCCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRAQYVLAENVTGMVHGPEGLAAAKRITDSLFSGDLHDMTEADFAQLAQDGMPTVELNRD
CCCEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCC
ADLQQALVNAELVPSRGQARTMIGSNAVAINGEKQADPEYVFTDADRLFGRYTLLRRGKK
CHHHHHHHCCCCCCCCCCCEEEECCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHCCCC
HYCLISWL
CEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA