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The map label for this gene is holB [H]

Identifier: 22125659

GI number: 22125659

Start: 1946755

End: 1947804

Strand: Direct

Name: holB [H]

Synonym: y1765

Alternate gene names: 22125659

Gene position: 1946755-1947804 (Clockwise)

Preceding gene: 22125658

Following gene: 22125660

Centisome position: 42.31

GC content: 52.48

Gene sequence:

>1050_bases
GTGGCTGACCAATCAGGAACCCGTGTAATGAATTGGTATCCGTGGCTTAACGCCCCTTACCGCCAACTGGTTGGGCAGCA
CAGTACCGGGCGCGGGCACCATGCATTACTGCTGCATTCATTACCCGGTAATGGCGAAGACGCATTGATTTATGCGCTGA
GTCGTTGGCTAATGTGTCAGCAGCGGCAAGGTGAGAAGAGTTGTGGTGAATGCCATAGCTGCCGCTTGATGTTGGCAGGC
AACCACCCCGATTGGTATGTACTGACACCGGAAAAAGGCAAAAGCAGTATCGGGGTTGAGTTGGTTCGTCAATTGATTGA
TAAACTGTATTCCCATGCTCAACAAGGCGGTGCAAAGGTGGTGTGGTTGCCACATGCCGAAGTGTTGACCGATGCCGCTG
CTAATGCCCTGCTAAAAACGCTGGAAGAGCCTCCAGAGAAGACCTATTTTCTGTTGGATTGCCACCAACCCGCCAGCTTG
CTGGCTACACTGCGTAGCCGCTGTTTTTATTGGTATCTGGCTTGCCCGGATACGGCTATTTGTCTGCAATGGCTCAATCT
GCAATGGCGTAAGCGGCAGATCCCTGTGGAGCCCGTTGCGATGTTGGCGGCCCTGAAATTGAGTGAAGGCGCACCGCTGG
CGGCTGAGCGGTTGCTCCAACCAGAGCGTTGGAGTATCCGCAGCGCTTTGTGTAGCGGGCTACGCGAGGCACTGAACCGC
TCAGACTTGCTGTCGTTGTTACCACAACTTAACCATGATGATGCTGCTGAACGTCTGCAATGGCTCTCTTCACTGCTCTT
GGATGCGTTGAAGTGGCAGCAAGGGGCAGGGGAGTTTGCGGTTAATCAGGATCAATTACCCTTAGTGCAACAGTTGGCAC
ACATCGCGGCGACGCCAGTATTACTTCAGTTGGCTAAGCAACTGGCTCATTGCCGCCATCAATTATTATCTGTGGTAGGT
GTTAATCGTGAATTGTTACTCACTGAGCAACTGCTTAGCTGGGAAACAGCACTGTCCACGGGTACTTATTCCACCTTACC
TTCGTTGTAA

Upstream 100 bases:

>100_bases
GTTACCTTGAATTGGCCGCCAGTGATGCCAGTATTAAAACGATTGATGCCTCCCAACCCATTGAACAAGTTAGTGCGTCG
ATAAGCCAGGCGCTCGCTCA

Downstream 100 bases:

>100_bases
AAGAGTTTAATATTATGTTGTTAGTTGATTCTCATTGCCATCTTGATAGCCTTGATTACCAGACATTGCACAGCAGTGTC
GATGACGTATTAGCCAAAGC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MADQSGTRVMNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCGECHSCRLMLAG
NHPDWYVLTPEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASL
LATLRSRCFYWYLACPDTAICLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNR
SDLLSLLPQLNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPVLLQLAKQLAHCRHQLLSVVG
VNRELLLTEQLLSWETALSTGTYSTLPSL

Sequences:

>Translated_349_residues
MADQSGTRVMNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCGECHSCRLMLAG
NHPDWYVLTPEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASL
LATLRSRCFYWYLACPDTAICLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNR
SDLLSLLPQLNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPVLLQLAKQLAHCRHQLLSVVG
VNRELLLTEQLLSWETALSTGTYSTLPSL
>Mature_348_residues
ADQSGTRVMNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCGECHSCRLMLAGN
HPDWYVLTPEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASLL
ATLRSRCFYWYLACPDTAICLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNRS
DLLSLLPQLNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPVLLQLAKQLAHCRHQLLSVVGV
NRELLLTEQLLSWETALSTGTYSTLPSL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=340, Percent_Identity=50.8823529411765, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1786676, Length=207, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39061; Mature: 38929

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQSGTRVMNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQ
CCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
QRQGEKSCGECHSCRLMLAGNHPDWYVLTPEKGKSSIGVELVRQLIDKLYSHAQQGGAKV
HHCCCCCCCCHHHCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
VWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCFYWYLACPDTAI
EEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHEEEEEECCCHHH
CLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNR
HHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCH
SDLLSLLPQLNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
LLQLAKQLAHCRHQLLSVVGVNRELLLTEQLLSWETALSTGTYSTLPSL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ADQSGTRVMNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQ
CCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
QRQGEKSCGECHSCRLMLAGNHPDWYVLTPEKGKSSIGVELVRQLIDKLYSHAQQGGAKV
HHCCCCCCCCHHHCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
VWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCFYWYLACPDTAI
EEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHEEEEEECCCHHH
CLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNR
HHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCH
SDLLSLLPQLNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
LLQLAKQLAHCRHQLLSVVGVNRELLLTEQLLSWETALSTGTYSTLPSL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430; 10491164 [H]