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The map label for this gene is menE [H]

Identifier: 22125559

GI number: 22125559

Start: 1838811

End: 1840277

Strand: Direct

Name: menE [H]

Synonym: y1664

Alternate gene names: 22125559

Gene position: 1838811-1840277 (Clockwise)

Preceding gene: 22125558

Following gene: 22125560

Centisome position: 39.97

GC content: 57.94

Gene sequence:

>1467_bases
GTGGCCAGACAGCCCGTTGCCGCTAATCACGACCGAGCAGTTGGGCGTGGTATGGCATCGTTAGGCCATCCGCCTTGGGA
GCATTGGCCTTGGCAGTATTGGGCCAATCTGCGGCCTCAAGCGCTCGCTATCCGTTCTGGGGGGCAACGGATCAGTTGGC
AACAGTTAGCCAGTGATATCAATGCGTTGGCGGCTTTTTTTCAACAGCAAGGTGTGGTGCCGGGGAGCGGCGTGGCCTTG
CGCGGTAAAAACAGTTATACCCTGCTATTGGCTTATCTGGCGGCATTGCAGTGTGCAGCTCGCGTTTTGCCGCTGAATCC
GCAATTGCCGGATGCGCTTTTGGCGCAGCGCCTGCCAACGCTGGATATTCACTTTATGCTGAATTTAGCCACCCCGTTGC
CCCCGCATTTTGCCTTTACTCCGTTGGATCCCCGCACCGTTGATTCGGCAGCCGTGACCCCGATAGCGTGGGATGGCCAA
CGGCTGGCGACGCTGACGCTAACCTCAGGTTCCTCCGGCTTACCGAAGGCCGCAGTACACTCGCTGGGCGCACATCGGGC
TAATGCCGACGGCGTACTGGCGCTGATGAATTTCACCGCCAGTGATAGCTGGTTGCTGTCGTTACCGTTGTTTCATGTCT
CAGGGCAGGGGATTGTCTGGCGTTGGCTGACGGCGGGGGCGACACTGGGGGTACCAGAGGGCGTGCCACTGGTTGAGGCT
TTGGCGGGGTGCAGCCATGCGTCATTAGTTCCCACGCAATTATGGCGTTTGTTGGCGAATGCCGCCCCCCCCTTGACGTT
GAAAGAGGTACTGTTGGGGGGCGCGATGATCCCAACCGAACTGACCGAACAGGCCGAGGCCCACGGCATCCGCTGTTGGT
GTGGCTACGGCTTGACGGAGTTGGCATCAACGGTCTGTGCCAAGCGGGCAGACGGGCGACCTGGTGTCGGTGTCGCGCTC
GCAGGCCGTCAGGTGAAACTGGTAGACGATGAAGTCTGGATAAAAGCCGATTGTCTGGCTGCCGGTTACTGGCAACAGGG
GCAATTACTGCCACTCACGGACAGCGACGGGTGGTTCCATACCCGAGATCGCGGTAGGTGGCAGCAAGGCGAATTATGTA
TTCTCGGGCGGTTGGATAATCTGTTTTTCAGTGGTGGCGAGGGGATCCAGCCAGAAGACATCGAGCGAGTTTTATTGCAG
TATTCAGGGGTGCAGCACGCTTTTGTTATTCCTGTTGCTGACGCTGAATTCGGTCACCGGCCAGTGGCGGTCATTGATTC
CGCTGATGAGGTGGATGTCGTGGCTTTAGCTGATTGGTTAGCCCCACGATTGGCTGTCTTCCAGCGGCCCATTGCTTTTT
ACCGGCTACCCACATTTTTGAAGCGTGGCGGGATTAAGATCTCAAGGCAGCAACTTGCCGACTGGCTGCGCGGCAAGTAT
GAAGGGGAGAAGATTGGCGAAAGATAG

Upstream 100 bases:

>100_bases
TGGGTCTGACGCAATTGGCCCGTTTGGCCGCTTGGTTGACCCCCGTCACGGTTCCGGGGTTGGATACGCTGGACCTGATG
CAGGCGCAAGTCGTGCGGCC

Downstream 100 bases:

>100_bases
AGCATGTCAGGCAAACTCAGCCCAGTTTATTGTTAACGAGAGACCCATAGGGGGAAGGCCGCACCATGGATTACAGTAAA
ATTATCAAAGAGGTTGGGCG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MARQPVAANHDRAVGRGMASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINALAAFFQQQGVVPGSGVAL
RGKNSYTLLLAYLAALQCAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHFAFTPLDPRTVDSAAVTPIAWDGQ
RLATLTLTSGSSGLPKAAVHSLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEA
LAGCSHASLVPTQLWRLLANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVGVAL
AGRQVKLVDDEVWIKADCLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERVLLQ
YSGVQHAFVIPVADAEFGHRPVAVIDSADEVDVVALADWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKY
EGEKIGER

Sequences:

>Translated_488_residues
MARQPVAANHDRAVGRGMASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINALAAFFQQQGVVPGSGVAL
RGKNSYTLLLAYLAALQCAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHFAFTPLDPRTVDSAAVTPIAWDGQ
RLATLTLTSGSSGLPKAAVHSLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEA
LAGCSHASLVPTQLWRLLANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVGVAL
AGRQVKLVDDEVWIKADCLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERVLLQ
YSGVQHAFVIPVADAEFGHRPVAVIDSADEVDVVALADWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKY
EGEKIGER
>Mature_487_residues
ARQPVAANHDRAVGRGMASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINALAAFFQQQGVVPGSGVALR
GKNSYTLLLAYLAALQCAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHFAFTPLDPRTVDSAAVTPIAWDGQR
LATLTLTSGSSGLPKAAVHSLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEAL
AGCSHASLVPTQLWRLLANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVGVALA
GRQVKLVDDEVWIKADCLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERVLLQY
SGVQHAFVIPVADAEFGHRPVAVIDSADEVDVVALADWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKYE
GEKIGER

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=451, Percent_Identity=55.2106430155211, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI145693145, Length=451, Percent_Identity=23.7250554323725, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788107, Length=355, Percent_Identity=25.3521126760563, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786801, Length=437, Percent_Identity=25.4004576659039, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=353, Percent_Identity=29.4617563739377, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=452, Percent_Identity=21.9026548672566, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 52965; Mature: 52834

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQPVAANHDRAVGRGMASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHH
NALAAFFQQQGVVPGSGVALRGKNSYTLLLAYLAALQCAARVLPLNPQLPDALLAQRLPT
HHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCC
LDIHFMLNLATPLPPHFAFTPLDPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKAAVH
EEEEEEEEECCCCCCCCEECCCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCHHHHH
SLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEA
HHCCCCCCCCCEEEEEEECCCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHH
LAGCSHASLVPTQLWRLLANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTE
HHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCHHH
LASTVCAKRADGRPGVGVALAGRQVKLVDDEVWIKADCLAAGYWQQGQLLPLTDSDGWFH
HHHHHHHHCCCCCCCCEEEECCCEEEEECCCEEEEEEEEEECCCCCCCEEEEECCCCCEE
TRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERVLLQYSGVQHAFVIPVADAEFGHR
ECCCCCCCCCCEEEEEECCHHEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
PVAVIDSADEVDVVALADWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKY
CEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEHHHHHHHHCCCC
EGEKIGER
CCHHCCCC
>Mature Secondary Structure 
ARQPVAANHDRAVGRGMASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDI
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHH
NALAAFFQQQGVVPGSGVALRGKNSYTLLLAYLAALQCAARVLPLNPQLPDALLAQRLPT
HHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCC
LDIHFMLNLATPLPPHFAFTPLDPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKAAVH
EEEEEEEEECCCCCCCCEECCCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCHHHHH
SLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEA
HHCCCCCCCCCEEEEEEECCCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHH
LAGCSHASLVPTQLWRLLANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTE
HHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCHHH
LASTVCAKRADGRPGVGVALAGRQVKLVDDEVWIKADCLAAGYWQQGQLLPLTDSDGWFH
HHHHHHHHCCCCCCCCEEEECCCEEEEECCCEEEEEEEEEECCCCCCCEEEEECCCCCEE
TRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERVLLQYSGVQHAFVIPVADAEFGHR
ECCCCCCCCCCEEEEEECCHHEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
PVAVIDSADEVDVVALADWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKY
CEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEHHHHHHHHCCCC
EGEKIGER
CCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]