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The map label for this gene is gntR [H]

Identifier: 22125514

GI number: 22125514

Start: 1787503

End: 1788639

Strand: Direct

Name: gntR [H]

Synonym: y1619

Alternate gene names: 22125514

Gene position: 1787503-1788639 (Clockwise)

Preceding gene: 22125511

Following gene: 22125518

Centisome position: 38.85

GC content: 48.55

Gene sequence:

>1137_bases
ATGCAGCTTGATGTATGGCCGGTATCGCGTGATATGGCCGGTATCGCGTTACATTGTCGAAGCCCGTTCGAATGGCCAGG
TTTCAGCAGACAGGGAATCAGCATGGAAAAAATACGCAAGCGCCGTAATACCGGGCGGGTCACGCTACAGGAAGTAGCCG
ATTATGCTGGGGTAGGCTCGATGACTGTTTCCCGAGCGCTACGAACGCCGGAACAGGTCTCAGATAAGTTACGTGAGAAA
ATAGAGCAAGCGGTTGAAAAATTAGGCTACATTCCAAACCGTGCCGCCGGTGCTTTAGCGTCAGGGCACAGCGATACCGT
GGCGGTGTTGATTCCGTCTTTAACCGATAAAGCCAGCTCTCGGTTTATGCAGGCACTACAGCAAATTCTCAATAAAAATG
AATTTCAGTTATTGCTGGGTTGCCATGAATACAATCAACGTAAAGAAGCTGAGATTCTTATGACGTTACTGCAAAGCCAC
CCGGCGGCAGTGGTGATATTTGGCTCGCAGTTGGCGGATAAGACATATCAGATTCTTGAGCGCACCAATATCCCTATCGT
TAATGTTGTCGGCTCCTATTTTAAAGGGGCTAAAATTACCCTTGAAGCGGCTTTCTTTGAATCGGCTTATGAATTAACCC
GTCACTTACTGGAGCGTGGCTATCAACATATTGGCTATCTGGGGGCGCATCGGGATAATCAGTTGCAACGCCAGCAGCTC
AATGGATGGCATAAGTCGATGCTCGAGCATTACAAAAATGCTGACCAAACGGTCACCACGCCCGATGCGGCCAGCTTACA
ATTTGGCCGTTATGCCTTGACGGAGATCTTACTGCGCCAGCCTGAATTAGATGCAGTGATATGCAGCCACGAAGATATTG
CACTTGGTGTGCTGTTTGAATGCCAGCGGCGGCTGCTTAAAATCCCTAGCGGCATTGCGGTTGCTTGCCTGGATGGCTCA
GAGAGCTGTGATCAAACTCATCCAACGCTGACCTCAATGCGTATCGATTATAAAAAAATGGGGAAAGAGGCCGGTAAGAT
GCTGATTGATATGCTCAATGAGGATGATGCCGGAGTGAAAAGTGTGCCCCTCAGCCAAAGTTTTAACTATAAATTTGAAC
CACGGCAAAGTACCTAA

Upstream 100 bases:

>100_bases
ATATATATTCTAAATAATTTGAGTGGTAGAAAGGCTGCTTACCGCAGCTTCGTCGAGGAAAGGTCATAAACGGGCCGAGT
AACAAGCGCAGCCAGCGCAG

Downstream 100 bases:

>100_bases
AGAGGAAAACCGCCCCAGAATGCAGGGCGGCTCCCTGTTTTACGTGTCTGATGTAACAAAAACGCCAGCGCAGAGGCTGG
CGTTTCAGACTGCTGACAAA

Product: LacI-family transcriptional regulatory protein

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MQLDVWPVSRDMAGIALHCRSPFEWPGFSRQGISMEKIRKRRNTGRVTLQEVADYAGVGSMTVSRALRTPEQVSDKLREK
IEQAVEKLGYIPNRAAGALASGHSDTVAVLIPSLTDKASSRFMQALQQILNKNEFQLLLGCHEYNQRKEAEILMTLLQSH
PAAVVIFGSQLADKTYQILERTNIPIVNVVGSYFKGAKITLEAAFFESAYELTRHLLERGYQHIGYLGAHRDNQLQRQQL
NGWHKSMLEHYKNADQTVTTPDAASLQFGRYALTEILLRQPELDAVICSHEDIALGVLFECQRRLLKIPSGIAVACLDGS
ESCDQTHPTLTSMRIDYKKMGKEAGKMLIDMLNEDDAGVKSVPLSQSFNYKFEPRQST

Sequences:

>Translated_378_residues
MQLDVWPVSRDMAGIALHCRSPFEWPGFSRQGISMEKIRKRRNTGRVTLQEVADYAGVGSMTVSRALRTPEQVSDKLREK
IEQAVEKLGYIPNRAAGALASGHSDTVAVLIPSLTDKASSRFMQALQQILNKNEFQLLLGCHEYNQRKEAEILMTLLQSH
PAAVVIFGSQLADKTYQILERTNIPIVNVVGSYFKGAKITLEAAFFESAYELTRHLLERGYQHIGYLGAHRDNQLQRQQL
NGWHKSMLEHYKNADQTVTTPDAASLQFGRYALTEILLRQPELDAVICSHEDIALGVLFECQRRLLKIPSGIAVACLDGS
ESCDQTHPTLTSMRIDYKKMGKEAGKMLIDMLNEDDAGVKSVPLSQSFNYKFEPRQST
>Mature_378_residues
MQLDVWPVSRDMAGIALHCRSPFEWPGFSRQGISMEKIRKRRNTGRVTLQEVADYAGVGSMTVSRALRTPEQVSDKLREK
IEQAVEKLGYIPNRAAGALASGHSDTVAVLIPSLTDKASSRFMQALQQILNKNEFQLLLGCHEYNQRKEAEILMTLLQSH
PAAVVIFGSQLADKTYQILERTNIPIVNVVGSYFKGAKITLEAAFFESAYELTRHLLERGYQHIGYLGAHRDNQLQRQQL
NGWHKSMLEHYKNADQTVTTPDAASLQFGRYALTEILLRQPELDAVICSHEDIALGVLFECQRRLLKIPSGIAVACLDGS
ESCDQTHPTLTSMRIDYKKMGKEAGKMLIDMLNEDDAGVKSVPLSQSFNYKFEPRQST

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=304, Percent_Identity=32.8947368421053, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=29.4117647058824, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1790369, Length=326, Percent_Identity=23.0061349693252, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789202, Length=298, Percent_Identity=25.8389261744966, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788474, Length=305, Percent_Identity=26.8852459016393, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786540, Length=319, Percent_Identity=25.3918495297806, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=24.3243243243243, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787948, Length=309, Percent_Identity=22.9773462783171, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787906, Length=255, Percent_Identity=25.0980392156863, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790194, Length=303, Percent_Identity=22.7722772277228, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787580, Length=318, Percent_Identity=22.9559748427673, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786268, Length=285, Percent_Identity=25.6140350877193, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790689, Length=308, Percent_Identity=23.7012987012987, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 42429; Mature: 42429

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLDVWPVSRDMAGIALHCRSPFEWPGFSRQGISMEKIRKRRNTGRVTLQEVADYAGVGS
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHCCCCH
MTVSRALRTPEQVSDKLREKIEQAVEKLGYIPNRAAGALASGHSDTVAVLIPSLTDKASS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCHHHHHHH
RFMQALQQILNKNEFQLLLGCHEYNQRKEAEILMTLLQSHPAAVVIFGSQLADKTYQILE
HHHHHHHHHHCCCCEEEEEEHHHHCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHH
RTNIPIVNVVGSYFKGAKITLEAAFFESAYELTRHLLERGYQHIGYLGAHRDNQLQRQQL
HCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NGWHKSMLEHYKNADQTVTTPDAASLQFGRYALTEILLRQPELDAVICSHEDIALGVLFE
HHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHH
CQRRLLKIPSGIAVACLDGSESCDQTHPTLTSMRIDYKKMGKEAGKMLIDMLNEDDAGVK
HHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
SVPLSQSFNYKFEPRQST
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQLDVWPVSRDMAGIALHCRSPFEWPGFSRQGISMEKIRKRRNTGRVTLQEVADYAGVGS
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHCCCCH
MTVSRALRTPEQVSDKLREKIEQAVEKLGYIPNRAAGALASGHSDTVAVLIPSLTDKASS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCHHHHHHH
RFMQALQQILNKNEFQLLLGCHEYNQRKEAEILMTLLQSHPAAVVIFGSQLADKTYQILE
HHHHHHHHHHCCCCEEEEEEHHHHCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHH
RTNIPIVNVVGSYFKGAKITLEAAFFESAYELTRHLLERGYQHIGYLGAHRDNQLQRQQL
HCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NGWHKSMLEHYKNADQTVTTPDAASLQFGRYALTEILLRQPELDAVICSHEDIALGVLFE
HHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHH
CQRRLLKIPSGIAVACLDGSESCDQTHPTLTSMRIDYKKMGKEAGKMLIDMLNEDDAGVK
HHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
SVPLSQSFNYKFEPRQST
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]