Click here to switch to the map view.

The map label for this gene is mepA [H]

Identifier: 22125481

GI number: 22125481

Start: 1753325

End: 1754194

Strand: Direct

Name: mepA [H]

Synonym: y1586

Alternate gene names: 22125481

Gene position: 1753325-1754194 (Clockwise)

Preceding gene: 22125480

Following gene: 22125482

Centisome position: 38.11

GC content: 53.45

Gene sequence:

>870_bases
GTGCGGCGATGTGGCTTCAGACGTTCCGCGCTGGTAGATAGCATGAAAAATTGGATAGTGGGTATGGTGGCATTGGTCAC
CATGGTCCCTGTCATGGCGGCCACGCCATGGCAAAAGATTACGCACCCGGTGGCGGGTTCTCCACAGTCAATCGGTGGCT
TTGCTAATGGTTGCGTGATTGGCGCGCAGCCTTTGCCGCTCGAGTCTGCAGACTATCAAGTGATGCGTTCGGATCAGCGC
CGTTATTTTGGTCACCCTGATTTACTGAATTTTATTCACCGTCTCAGTGCACAAGCTCATCAACAACAATTGGGCACGGT
GTTGATTGGTGATATGGCGATGCCTGCAGGCGGGCGTTTTAGCAGTGGGCATGCCAGCCACCAGTCTGGTTTGGATGTGG
ATATTTGGCTACAACTGCCAAAGCAGCGTTGGAGCCAGCAGCAGTTATTGAAACCGCAGCCGATAGACTTGGTCGCGGTT
GATGGCAAGCGGGTGATCCCCGCACTTTGGCAGCCACAAATTGAGAGTTTGATCAAATTGGCCGCCAAAGATAACGATGT
CACGCGTATCTTCGTCAATCCGGCAATTAAAAAGCAGCTTTGTCTGGATGCAGGTGCCGATCGTCAATGGCTGCATAAAG
TCCGGCCGTGGTTTGCTCATCGTGCACACATGCATGTGCGCTTACGTTGTCCGGCCAATAGCCTAGAGTGTGTGGACCAA
GACACTCCACCGCCGGGCGATGGCTGTGGTGCTGAGCTGGAAAGCTGGTTCCAACCGCCTCCGCCAAGTGCTAAACCAGG
TAAAACCTTGCCACCGCCATTACCGCCTTCTTGTCAGGCTTTACTGGATAACCACTTCGCTACGGAATAA

Upstream 100 bases:

>100_bases
GAGGGCGTCATGACCCGTGTGTGGGTATCCGGGCGGTACCTATTGCAGAAGCGATGATGGCTATCGTACTTATGGATCAC
CTACTGCGCCAGCGCGCGCA

Downstream 100 bases:

>100_bases
GTCATGGATTGGTTTACCCTTGGTCCTGCCGTTTTGGGGATACTCTTTGCCGTTGCATTATTAGCCGGGTTCATTGATTC
CATTGCCGGCGGGGGCGGCT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRRCGFRRSALVDSMKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQR
RYFGHPDLLNFIHRLSAQAHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAV
DGKRVIPALWQPQIESLIKLAAKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQ
DTPPPGDGCGAELESWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE

Sequences:

>Translated_289_residues
MRRCGFRRSALVDSMKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQR
RYFGHPDLLNFIHRLSAQAHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAV
DGKRVIPALWQPQIESLIKLAAKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQ
DTPPPGDGCGAELESWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE
>Mature_289_residues
MRRCGFRRSALVDSMKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQR
RYFGHPDLLNFIHRLSAQAHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAV
DGKRVIPALWQPQIESLIKLAAKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQ
DTPPPGDGCGAELESWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=252, Percent_Identity=73.4126984126984, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 31929; Mature: 31929

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRCGFRRSALVDSMKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHCCCCCCCEEE
GAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHRLSAQAHQQQLGTVLIGDMAMPAGGRF
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCC
SSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKL
CCCCCCCCCCCCEEEEEECCHHHCCHHHCCCCCCCEEEEECCCCCCHHHCCHHHHHHHHH
AAKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQ
HHCCCCEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHCCC
DTPPPGDGCGAELESWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MRRCGFRRSALVDSMKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHCCCCCCCEEE
GAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHRLSAQAHQQQLGTVLIGDMAMPAGGRF
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCC
SSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKL
CCCCCCCCCCCCEEEEEECCHHHCCHHHCCCCCCCEEEEECCCCCCHHHCCHHHHHHHHH
AAKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQ
HHCCCCEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHCCC
DTPPPGDGCGAELESWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA