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The map label for this gene is ynfE [H]

Identifier: 22125416

GI number: 22125416

Start: 1679412

End: 1681844

Strand: Reverse

Name: ynfE [H]

Synonym: y1519

Alternate gene names: 22125416

Gene position: 1681844-1679412 (Counterclockwise)

Preceding gene: 22125419

Following gene: 22125415

Centisome position: 36.56

GC content: 47.18

Gene sequence:

>2433_bases
ATGTTGATGAATAACAATAAAACACCGCTCACCGGCTCACTGAGTCGTCGTGAATTTATTCAAACATCCGCCGTAGTGAC
CGGGGCTGCGGCAATTGCCGGTTCAATCGCCCTCCCATTCTCCGTCCATGCTGCGGCTTCAGAACATGCCCCCACGGCGG
CTGAAGAGAGCATTAAATACAGTGCTTGCCTGGTTAACTGTGGCAGCCGCTGCCCGCTAAAAGTTCATGTTAAAAACGGT
GAAATAATTAAAATTGCTAATGAAGCCATTTATGATGATTCCACCTTTGGCTTACACCAAATTCGCCCCTGCTTACGTGG
CCGTTCAGTACGATGGAAAACCTATAATCCGGATCGGGTTAAATATCCAATGTTACGAGTGGGTAAACGCGGTGAAGGTA
AATTCAAGCGTATCAGTTGGGATGAAGCCACAACTATCGTTGCGGATAATCTGAAAAAAACGATTGTTCAATACGGCAAC
GAAGCAATTTATTATCAATATGGCACTGGTTCAACAGGGGCTAATTTACAAGGCCGCAATGCCTGTAAACGTATGTTGAG
TTTGATCGGCGGTTATCTTGATCAGCACGGTACTTATTCTACCGCCCAAATCAATACTGTCATGCCTTATGTCTACGGCA
ACTTAGATGAAACGCTGCTGGACGAAATTAAAAATTCTGATCTGGTGGTGATGTTTGGTCACAATCTGGCAGAAACACGG
ATGTCAGGCGGTGGGCAATATATTGAAACGGTTCAGGCGTTGGGTCAAAGCCGAGCCAAAGTCATTATTATTGACCCACG
CATGACCGACAGTGTGACCACCCTTAACGCCGAATGGATCCCGATATTCCCAGGGACCGATGCGGCACTGGTCGCCGCGC
TGGGGCATGTTTTAATTAAAGAGAATCTGACTGACGAACGTTTCCTGCAACAATATTGTGTCGGTTGGGATGCCACCACA
TTGCCCTCATCGGCCCCAGCTAACGGATCGTATAAAGATTATATTTTAGGTCTGGGTGCTGACGAAACCGAAAAAACACC
AGAATGGGCCAGTGAGATCACCGGTATTTCACCAACACGTATTATTCAATTAGCCAGAGAACTGGGTAATGCCCGCGCAG
CCTGGATTTCACAGGGTTGGGGAATTCAACGCACAGCGAACGGGGAGCAAGCGTCCCTCTCGATTATGATGCTCCCATTA
ATGACCGGTAACATTGGCCGCGCCGGAACCAATACCGGTGCCTGGGGTGGCAACGTAAAATACCCGGTTCCTGGGTTCAG
CATTCCCAATCCAGTCAAAACGGCCATTCCTTGCTTTATGTGGACCGATGCTATTTTCCGCGGCACTGAAATGACCGCTA
AAAATGCCCATGTGAAAAATAAAGAAAAACTGGATACCAATATTAAGTTTCTCTGGAACTACGCCAGTAACGTTATAGCA
AATCAGCACTCTGATCTGAATAAAACCAATACTATTTTGCAAGATGAGTCATTGTGCGAATTTATTTTGGTCTGGGAAAA
CCATATGACCAATTCGGCGAAGTACGCAGATTTATTATTACCAGACGTCACGTCGGTTGAATCAAATGACCTGATTGATA
ACTCCTATGCCAGTGGTGCTTATCACTATGTCACCCGGATGCAAAATACCATTGAGCCGCTGTGGGAATGCCGCCCCTCA
TACGATGTATTAGCCGAGATCGCCACTAAATTAGGCGTGGGGGATGCCTTTACTGAAGGACATACCCAGCAAGAATGGAT
TGAAATCAGCTATAACAAAATGCGTGATAATAATCCGGCGCTGCCGCCTTTCAGTGATACCCATAACAAAGGCATTATTG
ACCGTATTTTTGCTGACAGTAGCCAATATATCGCGTTAAAAGATTTCCGCGATGACCCATTAAATAACCCGTTAAAAACC
CCATCAGGCAAGATTGAGATCTATTCAGAAGCACTGGCAACACTCGCACACGAGTGGCAGTTGACACCGGGTGACCGCAT
TACCGCGGTAGCTGAATATTGTCCGACCCCTGAGGGTGTCAGTGATCAAGAAACCCTAAAGAGCTACCCTCTACAAATGA
CCGGTTTCCATGTTAAAGGCCATACCCACTCAACCTATTACAATGTCGCGATGCTGCGCGAAGCGGTACCTCATCAATTC
TGGATGAACCCGATTGATGCACAGGCGCGTGGCATACAGCAAGGCGAGCGGGTCGAAATATTTAATAGCCGTGGCCGTAT
CCATATTGCGGTCAAAATTACTGAGCGGGTATTACCGGGGGTTATTGCTGTGCCACAAGGGGCATGGCGTAATCTCAACA
AGGACGGTATCGACGTGGGTGGCTGTATCAATACGTTGACGACACAACGCCCTTCGCCATTAGCGAAAGGTAATCCTCAA
CACACCAACCTCGTTGAAGTAAAACGTGCATAA

Upstream 100 bases:

>100_bases
AGCATTCCAGTTCTATACCCAAGTCATTGGCATTGTTGCTAACACCACGACAACGTCAAGTAAAAAAGGGATGTCCTGCT
CTTTTATTTCCGCAAGGATA

Downstream 100 bases:

>100_bases
GGAGCTGAATAATGAAGCAATATGGTTTTTTTGTTGACTCCACCAAGTGCACGGGGTGTAAAACCTGCCAAGTCAGTTGT
AAGGACGAAAAGAATCTGGA

Product: oxidoreductase, major subunit

Products: NA

Alternate protein names: DMSO reductase [H]

Number of amino acids: Translated: 810; Mature: 810

Protein sequence:

>810_residues
MLMNNNKTPLTGSLSRREFIQTSAVVTGAAAIAGSIALPFSVHAAASEHAPTAAEESIKYSACLVNCGSRCPLKVHVKNG
EIIKIANEAIYDDSTFGLHQIRPCLRGRSVRWKTYNPDRVKYPMLRVGKRGEGKFKRISWDEATTIVADNLKKTIVQYGN
EAIYYQYGTGSTGANLQGRNACKRMLSLIGGYLDQHGTYSTAQINTVMPYVYGNLDETLLDEIKNSDLVVMFGHNLAETR
MSGGGQYIETVQALGQSRAKVIIIDPRMTDSVTTLNAEWIPIFPGTDAALVAALGHVLIKENLTDERFLQQYCVGWDATT
LPSSAPANGSYKDYILGLGADETEKTPEWASEITGISPTRIIQLARELGNARAAWISQGWGIQRTANGEQASLSIMMLPL
MTGNIGRAGTNTGAWGGNVKYPVPGFSIPNPVKTAIPCFMWTDAIFRGTEMTAKNAHVKNKEKLDTNIKFLWNYASNVIA
NQHSDLNKTNTILQDESLCEFILVWENHMTNSAKYADLLLPDVTSVESNDLIDNSYASGAYHYVTRMQNTIEPLWECRPS
YDVLAEIATKLGVGDAFTEGHTQQEWIEISYNKMRDNNPALPPFSDTHNKGIIDRIFADSSQYIALKDFRDDPLNNPLKT
PSGKIEIYSEALATLAHEWQLTPGDRITAVAEYCPTPEGVSDQETLKSYPLQMTGFHVKGHTHSTYYNVAMLREAVPHQF
WMNPIDAQARGIQQGERVEIFNSRGRIHIAVKITERVLPGVIAVPQGAWRNLNKDGIDVGGCINTLTTQRPSPLAKGNPQ
HTNLVEVKRA

Sequences:

>Translated_810_residues
MLMNNNKTPLTGSLSRREFIQTSAVVTGAAAIAGSIALPFSVHAAASEHAPTAAEESIKYSACLVNCGSRCPLKVHVKNG
EIIKIANEAIYDDSTFGLHQIRPCLRGRSVRWKTYNPDRVKYPMLRVGKRGEGKFKRISWDEATTIVADNLKKTIVQYGN
EAIYYQYGTGSTGANLQGRNACKRMLSLIGGYLDQHGTYSTAQINTVMPYVYGNLDETLLDEIKNSDLVVMFGHNLAETR
MSGGGQYIETVQALGQSRAKVIIIDPRMTDSVTTLNAEWIPIFPGTDAALVAALGHVLIKENLTDERFLQQYCVGWDATT
LPSSAPANGSYKDYILGLGADETEKTPEWASEITGISPTRIIQLARELGNARAAWISQGWGIQRTANGEQASLSIMMLPL
MTGNIGRAGTNTGAWGGNVKYPVPGFSIPNPVKTAIPCFMWTDAIFRGTEMTAKNAHVKNKEKLDTNIKFLWNYASNVIA
NQHSDLNKTNTILQDESLCEFILVWENHMTNSAKYADLLLPDVTSVESNDLIDNSYASGAYHYVTRMQNTIEPLWECRPS
YDVLAEIATKLGVGDAFTEGHTQQEWIEISYNKMRDNNPALPPFSDTHNKGIIDRIFADSSQYIALKDFRDDPLNNPLKT
PSGKIEIYSEALATLAHEWQLTPGDRITAVAEYCPTPEGVSDQETLKSYPLQMTGFHVKGHTHSTYYNVAMLREAVPHQF
WMNPIDAQARGIQQGERVEIFNSRGRIHIAVKITERVLPGVIAVPQGAWRNLNKDGIDVGGCINTLTTQRPSPLAKGNPQ
HTNLVEVKRA
>Mature_810_residues
MLMNNNKTPLTGSLSRREFIQTSAVVTGAAAIAGSIALPFSVHAAASEHAPTAAEESIKYSACLVNCGSRCPLKVHVKNG
EIIKIANEAIYDDSTFGLHQIRPCLRGRSVRWKTYNPDRVKYPMLRVGKRGEGKFKRISWDEATTIVADNLKKTIVQYGN
EAIYYQYGTGSTGANLQGRNACKRMLSLIGGYLDQHGTYSTAQINTVMPYVYGNLDETLLDEIKNSDLVVMFGHNLAETR
MSGGGQYIETVQALGQSRAKVIIIDPRMTDSVTTLNAEWIPIFPGTDAALVAALGHVLIKENLTDERFLQQYCVGWDATT
LPSSAPANGSYKDYILGLGADETEKTPEWASEITGISPTRIIQLARELGNARAAWISQGWGIQRTANGEQASLSIMMLPL
MTGNIGRAGTNTGAWGGNVKYPVPGFSIPNPVKTAIPCFMWTDAIFRGTEMTAKNAHVKNKEKLDTNIKFLWNYASNVIA
NQHSDLNKTNTILQDESLCEFILVWENHMTNSAKYADLLLPDVTSVESNDLIDNSYASGAYHYVTRMQNTIEPLWECRPS
YDVLAEIATKLGVGDAFTEGHTQQEWIEISYNKMRDNNPALPPFSDTHNKGIIDRIFADSSQYIALKDFRDDPLNNPLKT
PSGKIEIYSEALATLAHEWQLTPGDRITAVAEYCPTPEGVSDQETLKSYPLQMTGFHVKGHTHSTYYNVAMLREAVPHQF
WMNPIDAQARGIQQGERVEIFNSRGRIHIAVKITERVLPGVIAVPQGAWRNLNKDGIDVGGCINTLTTQRPSPLAKGNPQ
HTNLVEVKRA

Specific function: Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=805, Percent_Identity=52.1739130434783, Blast_Score=824, Evalue=0.0,
Organism=Escherichia coli, GI87081797, Length=810, Percent_Identity=51.1111111111111, Blast_Score=806, Evalue=0.0,
Organism=Escherichia coli, GI171474008, Length=815, Percent_Identity=49.3251533742331, Blast_Score=780, Evalue=0.0,
Organism=Escherichia coli, GI145693196, Length=758, Percent_Identity=30.8707124010554, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI87081994, Length=864, Percent_Identity=28.7037037037037, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1787231, Length=757, Percent_Identity=27.2126816380449, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI3868721, Length=780, Percent_Identity=24.4871794871795, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1788534, Length=425, Percent_Identity=23.7647058823529, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011888
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 89448; Mature: 89448

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMNNNKTPLTGSLSRREFIQTSAVVTGAAAIAGSIALPFSVHAAASEHAPTAAEESIKY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCEEEECCCCCCCCCHHHHHCEE
SACLVNCGSRCPLKVHVKNGEIIKIANEAIYDDSTFGLHQIRPCLRGRSVRWKTYNPDRV
EEEEEECCCCCCEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCE
KYPMLRVGKRGEGKFKRISWDEATTIVADNLKKTIVQYGNEAIYYQYGTGSTGANLQGRN
ECHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHH
ACKRMLSLIGGYLDQHGTYSTAQINTVMPYVYGNLDETLLDEIKNSDLVVMFGHNLAETR
HHHHHHHHHHHHHHCCCCCCEEEEEEECCHHCCCCCHHHHHHHCCCCEEEEECCCHHHHH
MSGGGQYIETVQALGQSRAKVIIIDPRMTDSVTTLNAEWIPIFPGTDAALVAALGHVLIK
HCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHH
ENLTDERFLQQYCVGWDATTLPSSAPANGSYKDYILGLGADETEKTPEWASEITGISPTR
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCHHH
IIQLARELGNARAAWISQGWGIQRTANGEQASLSIMMLPLMTGNIGRAGTNTGAWGGNVK
HHHHHHHHCCCCEEHHHCCCCCEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCC
YPVPGFSIPNPVKTAIPCFMWTDAIFRGTEMTAKNAHVKNKEKLDTNIKFLWNYASNVIA
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCEEEEHHHHHHHH
NQHSDLNKTNTILQDESLCEFILVWENHMTNSAKYADLLLPDVTSVESNDLIDNSYASGA
CCCCCCCHHHCEECCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCH
YHYVTRMQNTIEPLWECRPSYDVLAEIATKLGVGDAFTEGHTQQEWIEISYNKMRDNNPA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCCCCCHHEEEEHHHCCCCCCC
LPPFSDTHNKGIIDRIFADSSQYIALKDFRDDPLNNPLKTPSGKIEIYSEALATLAHEWQ
CCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHEE
LTPGDRITAVAEYCPTPEGVSDQETLKSYPLQMTGFHVKGHTHSTYYNVAMLREAVPHQF
CCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCHHH
WMNPIDAQARGIQQGERVEIFNSRGRIHIAVKITERVLPGVIAVPQGAWRNLNKDGIDVG
CCCCCCHHHHCCCCCCEEEEECCCCEEEEEEEEECHHCCCEEECCCHHHHCCCCCCCCCC
GCINTLTTQRPSPLAKGNPQHTNLVEVKRA
HHHHHHHCCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MLMNNNKTPLTGSLSRREFIQTSAVVTGAAAIAGSIALPFSVHAAASEHAPTAAEESIKY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCEEEECCCCCCCCCHHHHHCEE
SACLVNCGSRCPLKVHVKNGEIIKIANEAIYDDSTFGLHQIRPCLRGRSVRWKTYNPDRV
EEEEEECCCCCCEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCE
KYPMLRVGKRGEGKFKRISWDEATTIVADNLKKTIVQYGNEAIYYQYGTGSTGANLQGRN
ECHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHH
ACKRMLSLIGGYLDQHGTYSTAQINTVMPYVYGNLDETLLDEIKNSDLVVMFGHNLAETR
HHHHHHHHHHHHHHCCCCCCEEEEEEECCHHCCCCCHHHHHHHCCCCEEEEECCCHHHHH
MSGGGQYIETVQALGQSRAKVIIIDPRMTDSVTTLNAEWIPIFPGTDAALVAALGHVLIK
HCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHH
ENLTDERFLQQYCVGWDATTLPSSAPANGSYKDYILGLGADETEKTPEWASEITGISPTR
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCHHH
IIQLARELGNARAAWISQGWGIQRTANGEQASLSIMMLPLMTGNIGRAGTNTGAWGGNVK
HHHHHHHHCCCCEEHHHCCCCCEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCC
YPVPGFSIPNPVKTAIPCFMWTDAIFRGTEMTAKNAHVKNKEKLDTNIKFLWNYASNVIA
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCEEEEHHHHHHHH
NQHSDLNKTNTILQDESLCEFILVWENHMTNSAKYADLLLPDVTSVESNDLIDNSYASGA
CCCCCCCHHHCEECCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCH
YHYVTRMQNTIEPLWECRPSYDVLAEIATKLGVGDAFTEGHTQQEWIEISYNKMRDNNPA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCCCCCHHEEEEHHHCCCCCCC
LPPFSDTHNKGIIDRIFADSSQYIALKDFRDDPLNNPLKTPSGKIEIYSEALATLAHEWQ
CCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHEE
LTPGDRITAVAEYCPTPEGVSDQETLKSYPLQMTGFHVKGHTHSTYYNVAMLREAVPHQF
CCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCHHH
WMNPIDAQARGIQQGERVEIFNSRGRIHIAVKITERVLPGVIAVPQGAWRNLNKDGIDVG
CCCCCCHHHHCCCCCCEEEEECCCCEEEEEEEEECHHCCCEEECCCHHHHCCCCCCCCCC
GCINTLTTQRPSPLAKGNPQHTNLVEVKRA
HHHHHHHCCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]