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The map label for this gene is hisS [H]

Identifier: 22125253

GI number: 22125253

Start: 1505369

End: 1506643

Strand: Direct

Name: hisS [H]

Synonym: y1354

Alternate gene names: 22125253

Gene position: 1505369-1506643 (Clockwise)

Preceding gene: 22125252

Following gene: 22125254

Centisome position: 32.72

GC content: 50.82

Gene sequence:

>1275_bases
GTGGCAAAGAACATTCAAGCCATCCGTGGTATGAACGATTACCTGCCAGCCGATACGGCAATATGGCAGCGTATCGAAAG
CATCTTGAAGCAAGTGCTAAGCGGTTACGGTTACAGTGAAATCCGTATGCCGATTGTAGAGCAGACCCCGTTATTCAAAC
GCGCTATCGGTGAAGTGACCGATGTGGTTGAAAAAGAGATGTACACCTTTGATGATCGCAATGGCGAAAGTCTGACACTG
CGCCCGGAAGGGACTGCAGGCTGTGCGCGCGCGGGTATTGAACATGGTCTGCTGTACAATCAGGAACAGCGTCTGTGGTA
CATCGGTCCGATGTTCCGCTATGAACGTCCGCAAAAAGGTCGTTATCGTCAGTTCCATCAGTTGGGGGCCGAAGTCTTTG
GTCTGCCAGGGCCAGATATTGATGCCGAGCTGATTTTACTGACAGCCCGCTGGTGGCGTGCATTAGGTATCTTTGAACAT
GTGAAGTTGGAGCTGAACTCTATTGGTTCGTTGGCTGCCCGTGCTGACTATCGAGAAGCGCTGGTGGCGTTCCTGGAGCA
ACATGTTGAGGTATTGGACGAAGATTGTAAGCGCCGTATGTACAGCAATCCGTTGCGGGTGTTAGATTCCAAAAACCCTG
ATGTTCAGCAGTTGCTTGATGATGCGCCAAAACTGTCTGATTATCTGGATGAAGAGTCAAAACAACATTTTGCCGGGTTG
TGTGAACTTTTAGATAAGGCCAGCATCCCATATACCGTTAATGAGCGTTTAGTTCGTGGTTTAGATTATTACAACCGTAC
GGTATTTGAGTGGGTAACGCACAGCCTGGGCGCACAAGGCACAGTCTGTGCGGGTGGGCGTTATGATGGGTTGGTCGAGC
AACTCGGTGGTCGTGCAACACCGGCCGTGGGTTTTGCGATGGGCCTTGAGCGCTTGGTTCTGCTGGTGCAGGCTGTTAAT
GCTGATTTCCAGGTGCCTGCGACAGTTGATGCTTATGTTATCTCCTCCGGTGAGGGGGCGCAAAGTGCTGCGATGCTACT
TGCTGAGAGCCTACGTGATGCCTTACCAACGCTAAAAATAATGACCAATTACGGTGGCGGTAATGTTAAGAAACAATTTA
CACGCGCTGATAAGTGGGGCGCTCGTGTCGCTTTAATGCTGGGTGAAAGCGAAGTGGCCGCGCAGCAAGTCGTTGTAAAA
GATCTGCGAAATGGTGAACAAGAAACGCTGGCGCAAGCGGATGTTGCTGCGCGTCTGGCTTTGATGTTGGGTTAA

Upstream 100 bases:

>100_bases
ATTGCGCCAATAGAGTTAACGAGCCGTCCCCATTGAATATGCGGGCGGCAAGCCTCTATAATCGGCTTTATTTCTTGCAC
TAAAACTATAGAGAACTGAC

Downstream 100 bases:

>100_bases
GGAGAGAGACACCGTGGAAGTCTATACCACTGAAAATGACCAGGTTGATGCAGTGCGCCGTTTCTTTAGTGAAAATGGTA
AAGCACTGGCCGTGGGTGTG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAKNIQAIRGMNDYLPADTAIWQRIESILKQVLSGYGYSEIRMPIVEQTPLFKRAIGEVTDVVEKEMYTFDDRNGESLTL
RPEGTAGCARAGIEHGLLYNQEQRLWYIGPMFRYERPQKGRYRQFHQLGAEVFGLPGPDIDAELILLTARWWRALGIFEH
VKLELNSIGSLAARADYREALVAFLEQHVEVLDEDCKRRMYSNPLRVLDSKNPDVQQLLDDAPKLSDYLDEESKQHFAGL
CELLDKASIPYTVNERLVRGLDYYNRTVFEWVTHSLGAQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVN
ADFQVPATVDAYVISSGEGAQSAAMLLAESLRDALPTLKIMTNYGGGNVKKQFTRADKWGARVALMLGESEVAAQQVVVK
DLRNGEQETLAQADVAARLALMLG

Sequences:

>Translated_424_residues
MAKNIQAIRGMNDYLPADTAIWQRIESILKQVLSGYGYSEIRMPIVEQTPLFKRAIGEVTDVVEKEMYTFDDRNGESLTL
RPEGTAGCARAGIEHGLLYNQEQRLWYIGPMFRYERPQKGRYRQFHQLGAEVFGLPGPDIDAELILLTARWWRALGIFEH
VKLELNSIGSLAARADYREALVAFLEQHVEVLDEDCKRRMYSNPLRVLDSKNPDVQQLLDDAPKLSDYLDEESKQHFAGL
CELLDKASIPYTVNERLVRGLDYYNRTVFEWVTHSLGAQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVN
ADFQVPATVDAYVISSGEGAQSAAMLLAESLRDALPTLKIMTNYGGGNVKKQFTRADKWGARVALMLGESEVAAQQVVVK
DLRNGEQETLAQADVAARLALMLG
>Mature_423_residues
AKNIQAIRGMNDYLPADTAIWQRIESILKQVLSGYGYSEIRMPIVEQTPLFKRAIGEVTDVVEKEMYTFDDRNGESLTLR
PEGTAGCARAGIEHGLLYNQEQRLWYIGPMFRYERPQKGRYRQFHQLGAEVFGLPGPDIDAELILLTARWWRALGIFEHV
KLELNSIGSLAARADYREALVAFLEQHVEVLDEDCKRRMYSNPLRVLDSKNPDVQQLLDDAPKLSDYLDEESKQHFAGLC
ELLDKASIPYTVNERLVRGLDYYNRTVFEWVTHSLGAQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNA
DFQVPATVDAYVISSGEGAQSAAMLLAESLRDALPTLKIMTNYGGGNVKKQFTRADKWGARVALMLGESEVAAQQVVVKD
LRNGEQETLAQADVAARLALMLG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=352, Percent_Identity=27.2727272727273, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1788861, Length=424, Percent_Identity=81.8396226415094, Blast_Score=724, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47235; Mature: 47103

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNIQAIRGMNDYLPADTAIWQRIESILKQVLSGYGYSEIRMPIVEQTPLFKRAIGEVT
CCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCHHCCCHHHHHHHHHHH
DVVEKEMYTFDDRNGESLTLRPEGTAGCARAGIEHGLLYNQEQRLWYIGPMFRYERPQKG
HHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQLGAEVFGLPGPDIDAELILLTARWWRALGIFEHVKLELNSIGSLAARADYREA
HHHHHHHHHHHHCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAFLEQHVEVLDEDCKRRMYSNPLRVLDSKNPDVQQLLDDAPKLSDYLDEESKQHFAGL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
CELLDKASIPYTVNERLVRGLDYYNRTVFEWVTHSLGAQGTVCAGGRYDGLVEQLGGRAT
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHCCCCC
PAVGFAMGLERLVLLVQAVNADFQVPATVDAYVISSGEGAQSAAMLLAESLRDALPTLKI
CHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEE
MTNYGGGNVKKQFTRADKWGARVALMLGESEVAAQQVVVKDLRNGEQETLAQADVAARLA
EECCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LMLG
HHCC
>Mature Secondary Structure 
AKNIQAIRGMNDYLPADTAIWQRIESILKQVLSGYGYSEIRMPIVEQTPLFKRAIGEVT
CCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCHHCCCHHHHHHHHHHH
DVVEKEMYTFDDRNGESLTLRPEGTAGCARAGIEHGLLYNQEQRLWYIGPMFRYERPQKG
HHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQLGAEVFGLPGPDIDAELILLTARWWRALGIFEHVKLELNSIGSLAARADYREA
HHHHHHHHHHHHCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAFLEQHVEVLDEDCKRRMYSNPLRVLDSKNPDVQQLLDDAPKLSDYLDEESKQHFAGL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
CELLDKASIPYTVNERLVRGLDYYNRTVFEWVTHSLGAQGTVCAGGRYDGLVEQLGGRAT
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHCCCCC
PAVGFAMGLERLVLLVQAVNADFQVPATVDAYVISSGEGAQSAAMLLAESLRDALPTLKI
CHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEE
MTNYGGGNVKKQFTRADKWGARVALMLGESEVAAQQVVVKDLRNGEQETLAQADVAARLA
EECCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LMLG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA