Click here to switch to the map view.

The map label for this gene is mltF [H]

Identifier: 22125208

GI number: 22125208

Start: 1449970

End: 1451523

Strand: Reverse

Name: mltF [H]

Synonym: y1308

Alternate gene names: 22125208

Gene position: 1451523-1449970 (Counterclockwise)

Preceding gene: 22125210

Following gene: 22125204

Centisome position: 31.55

GC content: 49.03

Gene sequence:

>1554_bases
TTGTTGCGCAAAATGCCACTCCACTGGGGATTTAACCATGTTGAATTTAGCTTTACCGCACACACGAAGAGAGCGTTTCC
GAGAGATAACTATTTGACACGCATAAAATTAAGTTACTTTACCATCGGTCTGGTTGCTCTGCTTCTGGCGCTTGCGCTGT
GGCCCAATATTCCTTGGCGTAATGGGCAAGAGGGCCAACTTGACCAAATAAAAGCCCGTGGAGAACTGCGCGTCAGTACC
ATCAGTTCTCCTTTGATCTACTCTACCGAAAAGGATACCCCTTCCGGTTTTGACTATGAGCTAGCAAAACGTTTTGCTGA
CTATCTGGGGGTTAAACTGGTTATCATCCCCCATCATAATATTGACGATCTGTTTGACGCATTAGATAACGATGACACCG
ATCTGCTAGCCGCTGGGCTGATTTATAACCGTGAACGCCTCAATCGGGCGCGGACTGGCCCTGCCTACTATTCTGTTTCT
CAGCAATTGGTCTACCGGCTGGGTTCGCCACGGCCTAAATCGTTCAGCGATCTGAAAGGTCAGGTGGTTGTGGCCTCCGG
TTCGGCCCATATGACCACACTAAAACGGCTTAAGCAGACAAAATATCCTGAACTCAATTGGAGTTCATCCGTCGATAAGT
CCGGTAAAGAATTACTGGAGCAAGTCGCGGAAGGTAAGCTCGACTATACCCTTGGCGATTCAGCCACCATTGCCCTGTTA
CAACGCATTCATCCGCAATTGGCAGTCGCATTTGATGTCACCGATGAAGAGCCCGTCACCTGGTATTTTAAACAGAGTGA
TGACGATAGCCTGTATGCGGCCATGCTCGATTTCTACAGTGAGATGGTGGAAGACGGTAGCCTCGCACGTCTGGAGGAGA
AATATCTGGGTCATGTGGGGAGTTTTGACTATGTTGATACCAAAACTTTCCTGTCCGCTATCGATAATGTCCTGCCCTCT
TACCAGCACCTATTTGAAAAACATGCAGGGGACATTGACTGGAAATTGCTGGCCGTGATTGCCTATCAAGAATCACATTG
GAACCCGCAAGCCACCTCCCCTACTGGTGTTCGTGGCTTGATGATGCTAACCCGTGCGACCGCGGACGGGTTAGGGGTCA
AGGATAGGGTTGACCCTGAAGAAAGCATCAGAGGCGGGGCTATCTATTTGCAACGGCTGATGAAAAAACTCCCTGAAACC
ATCCCAGAAGATGAACGCATCTGGTTTGCACTGGCTGCGTATAATTTAGGCTATGGTCATATGCTGGATGCCCGCCGTTT
GACTAAAAATCAGAACGGTAACCCAGACAGTTGGGTTGATGTGAAAATGCGTTTACCGATGCTAAGCCAAAAACGCTACT
ACCCAAGCACCACTTACGGTTATGCCCGTGGTCATGAAGCCTATAACTATGTCGAGAATATTCGCCGTTATCAGGTCAGT
CTGGTGGGATATTTACAGGAAAAAGAGAAAAAAGCGGCTCAACATGCCGCCATTGAGGCGGAGCTGGGCAAAAGCAACCC
GGTTGTCGGCCCCGGTTGGTCAATCGGTGACTAA

Upstream 100 bases:

>100_bases
GTTTGCGTATCTATTATTAATAATTCAGTCAGAAACAGCCGCTATGTGTGTGTAACGCAGACTCAGCAAATAGCTCGCAA
TAAAGTTGCACACTGGCGTT

Downstream 100 bases:

>100_bases
TTGCTCGTCAGTCACTCGTCGGCAGTCGTTCAACCTTAATTACACCGTGATTTAGTCACCAACCGCCGCCCGTCATAACA
TGCTGGGCGGCGCATCACCT

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MLRKMPLHWGFNHVEFSFTAHTKRAFPRDNYLTRIKLSYFTIGLVALLLALALWPNIPWRNGQEGQLDQIKARGELRVST
ISSPLIYSTEKDTPSGFDYELAKRFADYLGVKLVIIPHHNIDDLFDALDNDDTDLLAAGLIYNRERLNRARTGPAYYSVS
QQLVYRLGSPRPKSFSDLKGQVVVASGSAHMTTLKRLKQTKYPELNWSSSVDKSGKELLEQVAEGKLDYTLGDSATIALL
QRIHPQLAVAFDVTDEEPVTWYFKQSDDDSLYAAMLDFYSEMVEDGSLARLEEKYLGHVGSFDYVDTKTFLSAIDNVLPS
YQHLFEKHAGDIDWKLLAVIAYQESHWNPQATSPTGVRGLMMLTRATADGLGVKDRVDPEESIRGGAIYLQRLMKKLPET
IPEDERIWFALAAYNLGYGHMLDARRLTKNQNGNPDSWVDVKMRLPMLSQKRYYPSTTYGYARGHEAYNYVENIRRYQVS
LVGYLQEKEKKAAQHAAIEAELGKSNPVVGPGWSIGD

Sequences:

>Translated_517_residues
MLRKMPLHWGFNHVEFSFTAHTKRAFPRDNYLTRIKLSYFTIGLVALLLALALWPNIPWRNGQEGQLDQIKARGELRVST
ISSPLIYSTEKDTPSGFDYELAKRFADYLGVKLVIIPHHNIDDLFDALDNDDTDLLAAGLIYNRERLNRARTGPAYYSVS
QQLVYRLGSPRPKSFSDLKGQVVVASGSAHMTTLKRLKQTKYPELNWSSSVDKSGKELLEQVAEGKLDYTLGDSATIALL
QRIHPQLAVAFDVTDEEPVTWYFKQSDDDSLYAAMLDFYSEMVEDGSLARLEEKYLGHVGSFDYVDTKTFLSAIDNVLPS
YQHLFEKHAGDIDWKLLAVIAYQESHWNPQATSPTGVRGLMMLTRATADGLGVKDRVDPEESIRGGAIYLQRLMKKLPET
IPEDERIWFALAAYNLGYGHMLDARRLTKNQNGNPDSWVDVKMRLPMLSQKRYYPSTTYGYARGHEAYNYVENIRRYQVS
LVGYLQEKEKKAAQHAAIEAELGKSNPVVGPGWSIGD
>Mature_517_residues
MLRKMPLHWGFNHVEFSFTAHTKRAFPRDNYLTRIKLSYFTIGLVALLLALALWPNIPWRNGQEGQLDQIKARGELRVST
ISSPLIYSTEKDTPSGFDYELAKRFADYLGVKLVIIPHHNIDDLFDALDNDDTDLLAAGLIYNRERLNRARTGPAYYSVS
QQLVYRLGSPRPKSFSDLKGQVVVASGSAHMTTLKRLKQTKYPELNWSSSVDKSGKELLEQVAEGKLDYTLGDSATIALL
QRIHPQLAVAFDVTDEEPVTWYFKQSDDDSLYAAMLDFYSEMVEDGSLARLEEKYLGHVGSFDYVDTKTFLSAIDNVLPS
YQHLFEKHAGDIDWKLLAVIAYQESHWNPQATSPTGVRGLMMLTRATADGLGVKDRVDPEESIRGGAIYLQRLMKKLPET
IPEDERIWFALAAYNLGYGHMLDARRLTKNQNGNPDSWVDVKMRLPMLSQKRYYPSTTYGYARGHEAYNYVENIRRYQVS
LVGYLQEKEKKAAQHAAIEAELGKSNPVVGPGWSIGD

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=481, Percent_Identity=60.7068607068607, Blast_Score=595, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 58608; Mature: 58608

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKMPLHWGFNHVEFSFTAHTKRAFPRDNYLTRIKLSYFTIGLVALLLALALWPNIPWR
CCCCCCCCCCCCEEEEEEEEHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCC
NGQEGQLDQIKARGELRVSTISSPLIYSTEKDTPSGFDYELAKRFADYLGVKLVIIPHHN
CCCCCCHHHHHCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHCEEEEEECCCC
IDDLFDALDNDDTDLLAAGLIYNRERLNRARTGPAYYSVSQQLVYRLGSPRPKSFSDLKG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
QVVVASGSAHMTTLKRLKQTKYPELNWSSSVDKSGKELLEQVAEGKLDYTLGDSATIALL
EEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHH
QRIHPQLAVAFDVTDEEPVTWYFKQSDDDSLYAAMLDFYSEMVEDGSLARLEEKYLGHVG
HHHCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
SFDYVDTKTFLSAIDNVLPSYQHLFEKHAGDIDWKLLAVIAYQESHWNPQATSPTGVRGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCCCCCCCCCCHHHH
MMLTRATADGLGVKDRVDPEESIRGGAIYLQRLMKKLPETIPEDERIWFALAAYNLGYGH
HHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCH
MLDARRLTKNQNGNPDSWVDVKMRLPMLSQKRYYPSTTYGYARGHEAYNYVENIRRYQVS
HHHHHHHHCCCCCCCCCEEEEEEECCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LVGYLQEKEKKAAQHAAIEAELGKSNPVVGPGWSIGD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRKMPLHWGFNHVEFSFTAHTKRAFPRDNYLTRIKLSYFTIGLVALLLALALWPNIPWR
CCCCCCCCCCCCEEEEEEEEHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCC
NGQEGQLDQIKARGELRVSTISSPLIYSTEKDTPSGFDYELAKRFADYLGVKLVIIPHHN
CCCCCCHHHHHCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHCEEEEEECCCC
IDDLFDALDNDDTDLLAAGLIYNRERLNRARTGPAYYSVSQQLVYRLGSPRPKSFSDLKG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
QVVVASGSAHMTTLKRLKQTKYPELNWSSSVDKSGKELLEQVAEGKLDYTLGDSATIALL
EEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHH
QRIHPQLAVAFDVTDEEPVTWYFKQSDDDSLYAAMLDFYSEMVEDGSLARLEEKYLGHVG
HHHCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
SFDYVDTKTFLSAIDNVLPSYQHLFEKHAGDIDWKLLAVIAYQESHWNPQATSPTGVRGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCCCCCCCCCCHHHH
MMLTRATADGLGVKDRVDPEESIRGGAIYLQRLMKKLPETIPEDERIWFALAAYNLGYGH
HHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCH
MLDARRLTKNQNGNPDSWVDVKMRLPMLSQKRYYPSTTYGYARGHEAYNYVENIRRYQVS
HHHHHHHHCCCCCCCCCEEEEEEECCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LVGYLQEKEKKAAQHAAIEAELGKSNPVVGPGWSIGD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA