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The map label for this gene is miaB [H]

Identifier: 22125098

GI number: 22125098

Start: 1338920

End: 1340458

Strand: Reverse

Name: miaB [H]

Synonym: y1195

Alternate gene names: 22125098

Gene position: 1340458-1338920 (Counterclockwise)

Preceding gene: 22125101

Following gene: 22125097

Centisome position: 29.14

GC content: 48.15

Gene sequence:

>1539_bases
ATGACTACAATGCTCCCTAACGACGAAGCCTTTGCGACTCTCTACTACAACGGAGATTATCTACCACAACGGAGTACCGC
ACGGAACGAGGTGATTTTGATGATGAATAACCCGATGACTAAAAAACTGCATATCAAAACCTGGGGTTGCCAGATGAATG
AATACGATTCATCTAAAATGGCAGATCTCTTGGCCAGTACCCATGGCTACCAGTTAACCACCATCCCGGAAGAAGCGGAT
TTACTGCTGCTAAATACCTGTTCTATCCGGGAAAAAGCACAGGAAAAAGTATTCAGCCTGCTAGGGCAATGGAAATTATT
GAAAGAGAAAAACCCGCAGTTGATTATTGGGGTCGGTGGTTGCGTGGCGTCACAGGAAGGTGAACAATTGCGCCAGCGCG
CCCCCTGTGTCGATGTCATCTTCGGGCCACAAACGCTGCATCGTTTACCGGAAATGATTAACCACGTGCAAGGAACGAAT
AGCCCTGTCGTGGATATCAGTTTTCCCGAAATTGAAAAATTTGACCGTTTGCCAGAACCACGGGCTGAAGGCCCAACTGC
GTTTGTTTCGATCATGGAAGGCTGCAATAAATATTGTACATTCTGTGTCGTACCCTACACCCGAGGCGAGGAAGTCAGCC
GTCCAAGCGATGACATTCTTTTTGAAATCGCCCAGTTAGCCGCTCAAGGCGTACGTGAAGTCAACTTACTCGGCCAGAAT
GTTAACGCCTACCGCGGGGCAACCTACGATGGCGATATCTGTAGTTTTGCCGAGCTGTTGCGTTTGGTTGCGGCGATTGA
TGGTATCGATAGGGTACGATTTACCACCAGCCACCCCATTGAGTTTACTGACGATATCATTGATGTTTATCGCGATACGC
CTGAATTGGTGAGCTTCCTGCATTTGCCGGTACAAAGCGGTTCTGACCGTATTTTGACCATGATGAAGCGTGCCCATACC
GCCTTGGAATACAAAGCCATCATCCGCAAACTGCGTCAAGCACGCCCTGATATCCAGATAAGTTCTGATTTTATTGTCGG
CTTCCCTGGTGAAACCCAGCAGGATTTTGAACAGACCATGAAACTGGTCGCAGATATTCATTTTGATACCAGCTACAGCT
TTATTTACTCCCCGCGTCCCGGCACACCAGCGGCTGATTTACCCAACAATGTGTCCGAAGAGGAGAAAAAACAGCGGCTA
CATATCCTGCAACAACGCATTAGCCAACAGGCGATGGAAATTAGCCGTAAGATGGTAGGGACAGTACAGCGTGTTTTGGT
GGAGGGTACCTCTCGCAAAAATGTCATGGAACTGGCCGGGCGTACGGAAAACAACCGCGTCGTGAACTTTGAGGGTTCAC
CCGACATGATTGGTAAATTCGTTGATGTCGAAATTGTTAACGTTTATGCCAGTTCACTGCGCGGCATTTTACTGCGTACA
GAAGATCAGATGGACCTGCGAACCCACGAGTCACCGCAATCGGTGATTGCTCGCACCCGTAAAGAAAATGAAATTGGTGT
AGGTATTTATCAGCCTTAA

Upstream 100 bases:

>100_bases
CTTCTTGCATTAGCAAGGTTTTGTCTAACCGCAGTGTAACGGATTTTTTATCACTCAACAGAATAACTCAACGGTTCTGC
GGTGGTCATCGCAGAGGCAA

Downstream 100 bases:

>100_bases
CCAACATCCATTTTGGTAACGCCCGGCCTAACATCATTGATCTGACAGGTTTAGTTGATCCCGCCCGCGTAACGAGGCAC
TGCATTGTCAGTGCCTGTTT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MTTMLPNDEAFATLYYNGDYLPQRSTARNEVILMMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEAD
LLLLNTCSIREKAQEKVFSLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTN
SPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQN
VNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHT
ALEYKAIIRKLRQARPDIQISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQRL
HILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRT
EDQMDLRTHESPQSVIARTRKENEIGVGIYQP

Sequences:

>Translated_512_residues
MTTMLPNDEAFATLYYNGDYLPQRSTARNEVILMMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEAD
LLLLNTCSIREKAQEKVFSLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTN
SPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQN
VNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHT
ALEYKAIIRKLRQARPDIQISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQRL
HILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRT
EDQMDLRTHESPQSVIARTRKENEIGVGIYQP
>Mature_511_residues
TTMLPNDEAFATLYYNGDYLPQRSTARNEVILMMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADL
LLLNTCSIREKAQEKVFSLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNS
PVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNV
NAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTA
LEYKAIIRKLRQARPDIQISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQRLH
ILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTE
DQMDLRTHESPQSVIARTRKENEIGVGIYQP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=35.6846473029046, Blast_Score=283, Evalue=3e-76,
Organism=Homo sapiens, GI28872782, Length=492, Percent_Identity=35.5691056910569, Blast_Score=283, Evalue=4e-76,
Organism=Homo sapiens, GI93277076, Length=446, Percent_Identity=29.8206278026906, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=83.7552742616034, Blast_Score=840, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=404, Percent_Identity=30.1980198019802, Blast_Score=172, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=463, Percent_Identity=32.3974082073434, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=256, Percent_Identity=31.25, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356207, Length=441, Percent_Identity=36.281179138322, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI19922432, Length=472, Percent_Identity=29.8728813559322, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 57785; Mature: 57654

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMLPNDEAFATLYYNGDYLPQRSTARNEVILMMNNPMTKKLHIKTWGCQMNEYDSSKM
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCHHHH
ADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLLGQWKLLKEKNPQLIIGVGG
HHHHHHCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
CVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNSPVVDISFPEIEKFDRLPEP
CCCCCHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCCCC
RAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQN
CCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
VNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSHPIEFTDDIIDVYRDTPELVSFL
CCHHCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCCCCCHHHHHHHHHCCCHHHHHHH
HLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQISSDFIVGFPGETQQDFEQTM
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCHHHHHHHH
KLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQRLHILQQRISQQAMEISRKMVG
HHHHHHEECCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRT
HHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEEC
EDQMDLRTHESPQSVIARTRKENEIGVGIYQP
CCCCCCCCCCCHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
TTMLPNDEAFATLYYNGDYLPQRSTARNEVILMMNNPMTKKLHIKTWGCQMNEYDSSKM
CCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCHHHH
ADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLLGQWKLLKEKNPQLIIGVGG
HHHHHHCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
CVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNSPVVDISFPEIEKFDRLPEP
CCCCCHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCCCC
RAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQN
CCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
VNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSHPIEFTDDIIDVYRDTPELVSFL
CCHHCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCCCCCHHHHHHHHHCCCHHHHHHH
HLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQISSDFIVGFPGETQQDFEQTM
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCHHHHHHHH
KLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQRLHILQQRISQQAMEISRKMVG
HHHHHHEECCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRT
HHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEEC
EDQMDLRTHESPQSVIARTRKENEIGVGIYQP
CCCCCCCCCCCHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA