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The map label for this gene is clpB [H]

Identifier: 22124822

GI number: 22124822

Start: 1021277

End: 1023871

Strand: Direct

Name: clpB [H]

Synonym: y0914

Alternate gene names: 22124822

Gene position: 1021277-1023871 (Clockwise)

Preceding gene: 22124821

Following gene: 229013132

Centisome position: 22.2

GC content: 47.86

Gene sequence:

>2595_bases
TTGACCAATATTGGAGGTGTTATGCGTCTGGATCGTCTTACCAGTAAATTCCAGCTTGCCCTCGCCGATGCCCAGTCTTT
AGCTCTTGGGCGCGACAACCAGTTTATTGAACCGTTACATTTGATGAGCGCACTGCTTAATCAGGATGGGGGGACGGTTC
GCCCTTTATTGACCTCGGCAGGGATTAATGTTGCACGTTTACGTAGCGACATTGAGCAAGCTTTAGGTCGTCTTCCTCAA
GTTGAAGGCACCGGCGGTGATGTTCAACCATCAAACGAGCTGGTTCGTGTATTAAATCTTTGCGACAAATTAGCGCAGAA
ACGTTCGGATAAGTTTATCTCTTCCGAGCTGTTCGTCCTGGCCGTACTTGAAGATCGTGGCACGTTAACTGACATGTTAA
AGGCTGCAGGTGCAACCGTCGCTATAATAGATAAGGCAATTGAAACCATGCGTGGTGGTGACAAAGTCGATGATCAGGGT
GCTGAAGATCAGCGCCAGGCGTTGAAAAAATTCACTATCGACCTGACTGAGCGTGCCGAGCAAGGCAAACTCGATCCGGT
TATTGGCCGTGATGAAGAAATTCGCCGTACTATTCAGGTATTACAACGTCGAACCAAAAATAACCCAGTGTTGATTGGTG
AGCCTGGGGTGGGTAAAACCGCTATTGTGGAAGGTTTGGCGCAACGCATTGTGAACGGCGAAGTGCCAGAAGGCTTGAAG
CATAAGCGTGTACTCTCATTAGATATGGGGTCGCTGATTGCCGGTGCCAAATATCGTGGTGAATTTGAAGAGCGTTTGAA
AGGCGTACTGAACGACTTATCAAAACAAGAAGGCAGTGTGATCTTGTTTATTGATGAGTTGCATACCATGGTCGGTGCCG
GTAAAGGCGATGGGGCGATGGATGCCGGTAACATGCTGAAACCTGCATTAGCGCGAGGTGAGTTACATTGTGTGGGGGCC
ACTACCCTCGATGAATACCGTCAATATATAGAGAAAGATGCTGCGCTCGAGCGGCGTTTCCAAAAAGTGTATGTTGCGGA
ACCAACGGTAGAAGACACTATCGCTATTTTACGTGGGCTGAAAGAGCGCTACGAACTGCACCATCACGTGCAGATCACTG
ACCCAGCTATTGTGGCAGCCGCAACATTGTCACACCGGTATATTTCTGATCGTCAGTTACCGGATAAAGCTATCGACCTC
ATTGATGAGGCAGGTTCCAGCATCCGTATGCAAATGGATTCAAAACCCGAATCATTGGATCGTCTAGAGCGCCGGATTAT
TCAGCTTAAACTGGAACAGCAGGCCCTAAAAAAAGAGTCAGATGACGCCAGTAAAAAACGGTTGGAAATGCTCAATACTG
AGTTAGAGCAAAAAGAGCGTGAATATTCTGAACTGGAAGAAGAGTGGAAAGCAGAAAAAGCTTCACTCACCGGGACTCAG
AATATCAAGACGGAGTTGGAACAAGCTAAAATCACCTTAGAACAAGCTCGTCGAGTAGGTGATCTGGCTCGGATGTCAGA
ACTTCAGTACGGTACAATCCCTGAGCTGGAAAAACAGCTGGCGGCGGCAACCGCGCTAGAAGGGAAAACCATGAAGTTGC
TGCGTAACCGTGTCACAGAAATCGAAGTTGCTGAAGTCCTGGCTCGCTGGACCGGTATCCCGGTATCTCGCATGTTGGAA
AGTGAGCGGGATAAGCTGCTACGTATGGAACAGGACTTGCATCAACGAGTCATTGGGCAAGATGAAGCTGTTGAAGCCGT
CTCCAACGCTATCCGTCGGAGTCGCGCAGGGTTGTCTGATCCAAACCGACCAATTGGTTCGTTCTTATTCTTAGGGCCAA
CGGGGGTTGGTAAAACTGAATTGTGTAAAGCGTTGGCAACGTTCTTGTTCGACAGTGACGATGCCATGGTGCGAATCGAC
ATGTCCGAGTTTATGGAGAAACATTCCGTATCGCGGTTGGTCGGTGCACCTCCTGGCTATGTTGGGTATGAAGAAGGTGG
CTATCTGACGGAAGCGGTTCGTCGTCGTCCTTATTCTGTGATTTTGCTGGATGAAGTTGAAAAAGCACATCCAGATGTTT
TCAACATATTGTTACAGGTATTGGATGATGGTCGTTTGACCGACGGGCAGGGGAGAACTGTCGACTTTCGTAACACGGTA
GTCATTATGACCTCTAACCTTGGTTCAGATTTGATTCAGGAACATTTCGGCCAAATGAATTACAGCGAAATGAAGACAGT
GGTTATGGATGTTGTTAGTCACCACTTCCGTCCTGAATTTATTAACCGTATAGATGAGGTTGTTGTCTTCCACCCACTTG
GTCGGGCGCACTTGGCTTCTATTGCCAACATTCAACTGGAGCGTTTGTATAAACGTCTGGAAGAACGTGGCTATGAAGTG
AAGATTACTCAGCCTGCATTGGAATTCTTGGGTGAAGCTGGCTTCGACCCTGTTTATGGGGCGCGGCCATTAAAACGTGC
AATTCAGCAGGAAATTGAGAACCCGCTGGCACAGCAGATACTTTCTGGCAAACTAATACCGGGAATCCCCGTGACTTTAG
ATGTTGAAAATGGGCATATTATTGCTAAGCAGTAA

Upstream 100 bases:

>100_bases
GTCAGGACGATCCGCTGACGTAGAACGCATTATTTTAATATAGTGTCAAAATAACCTTGAAAAATTGAGGGATGACCTCA
TTTAATCTCCAGTAGCAATT

Downstream 100 bases:

>100_bases
TAGCTGATCCTCTAAGGGGCGAGAAATCGCCCCTTTTTTGTTGTTTTTTTCTTCATATATTGCATTTATGATGCATTAGT
GAGCGGTTGAAAAGTTTTTT

Product: protein disaggregation chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 864; Mature: 863

Protein sequence:

>864_residues
MTNIGGVMRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSAGINVARLRSDIEQALGRLPQ
VEGTGGDVQPSNELVRVLNLCDKLAQKRSDKFISSELFVLAVLEDRGTLTDMLKAAGATVAIIDKAIETMRGGDKVDDQG
AEDQRQALKKFTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLK
HKRVLSLDMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAMDAGNMLKPALARGELHCVGA
TTLDEYRQYIEKDAALERRFQKVYVAEPTVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDL
IDEAGSSIRMQMDSKPESLDRLERRIIQLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQ
NIKTELEQAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTEIEVAEVLARWTGIPVSRMLE
SERDKLLRMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALATFLFDSDDAMVRID
MSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTV
VIMTSNLGSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLASIANIQLERLYKRLEERGYEV
KITQPALEFLGEAGFDPVYGARPLKRAIQQEIENPLAQQILSGKLIPGIPVTLDVENGHIIAKQ

Sequences:

>Translated_864_residues
MTNIGGVMRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSAGINVARLRSDIEQALGRLPQ
VEGTGGDVQPSNELVRVLNLCDKLAQKRSDKFISSELFVLAVLEDRGTLTDMLKAAGATVAIIDKAIETMRGGDKVDDQG
AEDQRQALKKFTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLK
HKRVLSLDMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAMDAGNMLKPALARGELHCVGA
TTLDEYRQYIEKDAALERRFQKVYVAEPTVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDL
IDEAGSSIRMQMDSKPESLDRLERRIIQLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQ
NIKTELEQAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTEIEVAEVLARWTGIPVSRMLE
SERDKLLRMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALATFLFDSDDAMVRID
MSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTV
VIMTSNLGSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLASIANIQLERLYKRLEERGYEV
KITQPALEFLGEAGFDPVYGARPLKRAIQQEIENPLAQQILSGKLIPGIPVTLDVENGHIIAKQ
>Mature_863_residues
TNIGGVMRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSAGINVARLRSDIEQALGRLPQV
EGTGGDVQPSNELVRVLNLCDKLAQKRSDKFISSELFVLAVLEDRGTLTDMLKAAGATVAIIDKAIETMRGGDKVDDQGA
EDQRQALKKFTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKH
KRVLSLDMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAMDAGNMLKPALARGELHCVGAT
TLDEYRQYIEKDAALERRFQKVYVAEPTVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDLI
DEAGSSIRMQMDSKPESLDRLERRIIQLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQN
IKTELEQAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTEIEVAEVLARWTGIPVSRMLES
ERDKLLRMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALATFLFDSDDAMVRIDM
SEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVV
IMTSNLGSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLASIANIQLERLYKRLEERGYEVK
ITQPALEFLGEAGFDPVYGARPLKRAIQQEIENPLAQQILSGKLIPGIPVTLDVENGHIIAKQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=320, Percent_Identity=37.1875, Blast_Score=218, Evalue=3e-56,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=86.6977829638273, Blast_Score=1527, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=419, Percent_Identity=39.8568019093079, Blast_Score=280, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6320464, Length=710, Percent_Identity=52.9577464788732, Blast_Score=760, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=838, Percent_Identity=43.1980906921241, Blast_Score=693, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96320; Mature: 96189

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIGGVMRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHC
GINVARLRSDIEQALGRLPQVEGTGGDVQPSNELVRVLNLCDKLAQKRSDKFISSELFVL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AVLEDRGTLTDMLKAAGATVAIIDKAIETMRGGDKVDDQGAEDQRQALKKFTIDLTERAE
EEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCC
HKRVLSLDMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAM
CCEEEEECHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCC
DAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQKVYVAEPTVEDTIAILRGL
CCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KERYELHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAGSSIRMQMDSKPESLD
HHHHHHHHCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHH
RLERRIIQLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
NIKTELEQAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
IEVAEVLARWTGIPVSRMLESERDKLLRMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
PNRPIGSFLFLGPTGVGKTELCKALATFLFDSDDAMVRIDMSEFMEKHSVSRLVGAPPGY
CCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCC
VGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTV
CCCCCCCHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEE
VIMTSNLGSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLAS
EEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCHHHHHH
IANIQLERLYKRLEERGYEVKITQPALEFLGEAGFDPVYGARPLKRAIQQEIENPLAQQI
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LSGKLIPGIPVTLDVENGHIIAKQ
HCCCCCCCCCEEEEECCCEEEECC
>Mature Secondary Structure 
TNIGGVMRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHC
GINVARLRSDIEQALGRLPQVEGTGGDVQPSNELVRVLNLCDKLAQKRSDKFISSELFVL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AVLEDRGTLTDMLKAAGATVAIIDKAIETMRGGDKVDDQGAEDQRQALKKFTIDLTERAE
EEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCC
HKRVLSLDMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAM
CCEEEEECHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCC
DAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQKVYVAEPTVEDTIAILRGL
CCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KERYELHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAGSSIRMQMDSKPESLD
HHHHHHHHCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHH
RLERRIIQLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
NIKTELEQAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
IEVAEVLARWTGIPVSRMLESERDKLLRMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
PNRPIGSFLFLGPTGVGKTELCKALATFLFDSDDAMVRIDMSEFMEKHSVSRLVGAPPGY
CCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCC
VGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTV
CCCCCCCHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEE
VIMTSNLGSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLAS
EEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCHHHHHH
IANIQLERLYKRLEERGYEVKITQPALEFLGEAGFDPVYGARPLKRAIQQEIENPLAQQI
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LSGKLIPGIPVTLDVENGHIIAKQ
HCCCCCCCCCEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]