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The map label for this gene is rbsB [H]

Identifier: 22124782

GI number: 22124782

Start: 977336

End: 978325

Strand: Direct

Name: rbsB [H]

Synonym: y0871

Alternate gene names: 22124782

Gene position: 977336-978325 (Clockwise)

Preceding gene: 22124778

Following gene: 22124783

Centisome position: 21.24

GC content: 49.09

Gene sequence:

>990_bases
TTGAATATAAATTTAATGGTGAATATATATTCAATGCGGAGAAAAATAATGAAAAATACCCTACTGAAATCCTGTCTTAC
CGCAGCATTAATCTCAATGGCAGGCGTTGCTGGTGCGGCCAGCAATGGTCTGATCGCCATCATCACCCCCTCCCACGATA
ATCCGTTCTTTAAAGCAGAGGCTGAAGGTGCCAAAGCGAAAGCGACGGAACTGGGATACACCACACTGGTTGCTTCGCAT
GATGACGATGTGAACAAACAGAACCAACTCATTGAGACCGCTATCGCCCGTAAAGCCAAAGCGATCATTCTGGATAACGC
CGGCTCTGACGCGACCATCGGCCCATTGAAAAAAGCCAAAGCCGCGGGTATCCCGGCCTTCCTTATTGACCGTGAAATCA
ATGAAACCGGGATTGCCGTCTCTCAGATTGTCTCTAACAACTATCAGGGCGCGCAGCTGGGCGCGGAGAAGTTTGTCACT
CTGATGGGGGGCAAAGGTAAATATATTGAATTACTGGGGCGCGAGTCTGACACCAACGCTCATGTACGTTCGCAGGGTTA
TCACGACGTCATTGACGAACATAGCGACATGAAGATGGTTGCTCAACAGACAGCAAACTGGAGCCAGACTGAAGCATTCA
ACCGTATGGAATCTATTTTGCAGGCTAACCCAGATATTACAGGGGTCATTTCCGGTAACGACACGATGGCATTAGGGGCC
GAAGCCGCATTAAAAGCGGCGGGCCGCAATGATGTGGTGGTGGTGGGGTTTGATGGCAGTGACTATGTCCGCGACTCGAT
CATTAACAACGGCAATATCAAAGCCACCGTATTGCAGCCGGGTTGGGCGCAAGCACAAATGGCCGTTGTCCAGGCCGATC
AGTATCTGAAAACCGGTAAAACCGGTCTGGAAGAGAAACAACTGATGGACTGTGTATTGATTGACGAAAATAACGCTAAG
AACCTGAATGTCTTTGCGCTGAAAGACTAG

Upstream 100 bases:

>100_bases
TTTCTGAGACATCCCCTCTCCCTTATCGACTAATTTGTGACCGACTTCTACTTTCCTTGCTCTGACATTGGACAAGGCGT
AGGGCGTTTGTATTATTAGA

Downstream 100 bases:

>100_bases
CCGCTACAGGCGTGTTCGCTAAAGCCAGTGCGATAAAACATCCGCTTTAGCCGTAATGAGGGGCGACATCGCCCCTCCTT
TCAGGAGGCATTATGAGGTT

Product: solute-binding periplasmic protein of ABC transporter

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNINLMVNIYSMRRKIMKNTLLKSCLTAALISMAGVAGAASNGLIAIITPSHDNPFFKAEAEGAKAKATELGYTTLVASH
DDDVNKQNQLIETAIARKAKAIILDNAGSDATIGPLKKAKAAGIPAFLIDREINETGIAVSQIVSNNYQGAQLGAEKFVT
LMGGKGKYIELLGRESDTNAHVRSQGYHDVIDEHSDMKMVAQQTANWSQTEAFNRMESILQANPDITGVISGNDTMALGA
EAALKAAGRNDVVVVGFDGSDYVRDSIINNGNIKATVLQPGWAQAQMAVVQADQYLKTGKTGLEEKQLMDCVLIDENNAK
NLNVFALKD

Sequences:

>Translated_329_residues
MNINLMVNIYSMRRKIMKNTLLKSCLTAALISMAGVAGAASNGLIAIITPSHDNPFFKAEAEGAKAKATELGYTTLVASH
DDDVNKQNQLIETAIARKAKAIILDNAGSDATIGPLKKAKAAGIPAFLIDREINETGIAVSQIVSNNYQGAQLGAEKFVT
LMGGKGKYIELLGRESDTNAHVRSQGYHDVIDEHSDMKMVAQQTANWSQTEAFNRMESILQANPDITGVISGNDTMALGA
EAALKAAGRNDVVVVGFDGSDYVRDSIINNGNIKATVLQPGWAQAQMAVVQADQYLKTGKTGLEEKQLMDCVLIDENNAK
NLNVFALKD
>Mature_329_residues
MNINLMVNIYSMRRKIMKNTLLKSCLTAALISMAGVAGAASNGLIAIITPSHDNPFFKAEAEGAKAKATELGYTTLVASH
DDDVNKQNQLIETAIARKAKAIILDNAGSDATIGPLKKAKAAGIPAFLIDREINETGIAVSQIVSNNYQGAQLGAEKFVT
LMGGKGKYIELLGRESDTNAHVRSQGYHDVIDEHSDMKMVAQQTANWSQTEAFNRMESILQANPDITGVISGNDTMALGA
EAALKAAGRNDVVVVGFDGSDYVRDSIINNGNIKATVLQPGWAQAQMAVVQADQYLKTGKTGLEEKQLMDCVLIDENNAK
NLNVFALKD

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=293, Percent_Identity=31.0580204778157, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790526, Length=244, Percent_Identity=26.6393442622951, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790715, Length=262, Percent_Identity=25.5725190839695, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790674, Length=210, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35229; Mature: 35229

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNINLMVNIYSMRRKIMKNTLLKSCLTAALISMAGVAGAASNGLIAIITPSHDNPFFKAE
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEC
AEGAKAKATELGYTTLVASHDDDVNKQNQLIETAIARKAKAIILDNAGSDATIGPLKKAK
CCCCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHH
AAGIPAFLIDREINETGIAVSQIVSNNYQGAQLGAEKFVTLMGGKGKYIELLGRESDTNA
HCCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHCHHHEEEEECCCCCEEEEECCCCCCCC
HVRSQGYHDVIDEHSDMKMVAQQTANWSQTEAFNRMESILQANPDITGVISGNDTMALGA
CCCCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECH
EAALKAAGRNDVVVVGFDGSDYVRDSIINNGNIKATVLQPGWAQAQMAVVQADQYLKTGK
HHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCC
TGLEEKQLMDCVLIDENNAKNLNVFALKD
CCCCHHHHEEEEEEECCCCCEEEEEEECC
>Mature Secondary Structure
MNINLMVNIYSMRRKIMKNTLLKSCLTAALISMAGVAGAASNGLIAIITPSHDNPFFKAE
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEC
AEGAKAKATELGYTTLVASHDDDVNKQNQLIETAIARKAKAIILDNAGSDATIGPLKKAK
CCCCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHH
AAGIPAFLIDREINETGIAVSQIVSNNYQGAQLGAEKFVTLMGGKGKYIELLGRESDTNA
HCCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHCHHHEEEEECCCCCEEEEECCCCCCCC
HVRSQGYHDVIDEHSDMKMVAQQTANWSQTEAFNRMESILQANPDITGVISGNDTMALGA
CCCCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECH
EAALKAAGRNDVVVVGFDGSDYVRDSIINNGNIKATVLQPGWAQAQMAVVQADQYLKTGK
HHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCC
TGLEEKQLMDCVLIDENNAKNLNVFALKD
CCCCHHHHEEEEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]