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The map label for this gene is gutB [H]

Identifier: 22124749

GI number: 22124749

Start: 938941

End: 940089

Strand: Direct

Name: gutB [H]

Synonym: y0838

Alternate gene names: 22124749

Gene position: 938941-940089 (Clockwise)

Preceding gene: 22124747

Following gene: 22124750

Centisome position: 20.41

GC content: 48.91

Gene sequence:

>1149_bases
GTGTGCGATAAAAAACCGTTAAAGGAGTCCAGCATGAGTCACGAAAACCTTAAAGGCGCTCAAATGGCGGAGGGTATCCC
CGAGACCATGCGTGCAGTTGTCGCCTACGGGCCGCGCGATTATCGGTTTGAAAGAGTGCCGGTTCCGACTATCGATGCCA
AAGAGATTTTGGTCAAAGTTGAAGGGTGTGGCATCTGCGCCGGGGACACCAAGGCTTTTGAGGGTGCCCCCAGTTTCTGG
GGTGATGATAAGCAACCTGCTTATATCAAAGCACCGATGATTCCAGGTCATGAATTTATTGGTCACGTCGTTGGCTTGGG
CGCAGATGTTGAGGGGTTTGAGCTAGGTGATAGAGTGACTTCTGAGCAAATTGTTCCTTGCTGGGGCTGCCGGTTCTGTA
ACCATGGGCAGTACTGGATGTGTGAAAAACACGATCTGTACGGCTTCCAGAGCAATGTTAATGGCGCGATGGCTGAATAC
CTGAAATTCACGAAAGAAGCGATTAATTATCATGTCCCTGATCACCTTCCTTTAGAAAAAGCCATTCTGATTGAACCCTA
TGCCTGCGCATTTCACGCGGTACAACGCGCCAAGATAAAACTCGGTGATGTGGTGGTTTTGGCCGGTTCAGGAACCTTAG
GGCTAGGCATGATTGGGGCAATTAAAAAGTCTGGGGCTGCCAAGCTCATTGTGCTGGATTTATCGGATGATCGTTTGGCT
CTCGCCAAGAAGTTCGGCGCTGATTTGGTGCTTAATCCCAGTAGAGATGATGTGAATACCCTGATTAAAGAAATGACCGG
TGGTTATGGTTGCGATATCTACATTGATGCCACTGGTGCGCAAAAAGCCGTTGAACAAGGATTAACCCTGCTGCGTAAGT
TGGGGACGTTTGTGGAATTCTCGGTATTTAAAGATCCCGTGACCGTTGACTGGAGCATCATCAGTGACCGTAAGGAACTT
GATGTCTTGGGTTCACATCTGGGGCCATATTGCTTCCCGCTGGTGATTGAAGGCATCTCTAACGGTGATTTACCGACCGA
GGGGGTCGTCACACATATCTTGCCATTGGAGAAATATGCCGAGGGTATTGAGCTGGTCAAAAGAGGCGTTGACTCACTTA
AAGTGGTTCTCAATCCCAACCTAACCTAA

Upstream 100 bases:

>100_bases
GCGCTTGATCATACTGTTCAATAGTACGTAGTTAAATCTTATGTTCAATGCGTGTTTAAGATGAATTCAGATATCTGACA
AGACATTTATCACAAAACAC

Downstream 100 bases:

>100_bases
TCAGTGCTTATTTCCTGAGTCAGGGTGGATCACGAAGAAGGGGTATGAATAAGGAATATAAAGATGCCAAACGACTATAA
ATGGTTTGATACGGCGTTTT

Product: dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MCDKKPLKESSMSHENLKGAQMAEGIPETMRAVVAYGPRDYRFERVPVPTIDAKEILVKVEGCGICAGDTKAFEGAPSFW
GDDKQPAYIKAPMIPGHEFIGHVVGLGADVEGFELGDRVTSEQIVPCWGCRFCNHGQYWMCEKHDLYGFQSNVNGAMAEY
LKFTKEAINYHVPDHLPLEKAILIEPYACAFHAVQRAKIKLGDVVVLAGSGTLGLGMIGAIKKSGAAKLIVLDLSDDRLA
LAKKFGADLVLNPSRDDVNTLIKEMTGGYGCDIYIDATGAQKAVEQGLTLLRKLGTFVEFSVFKDPVTVDWSIISDRKEL
DVLGSHLGPYCFPLVIEGISNGDLPTEGVVTHILPLEKYAEGIELVKRGVDSLKVVLNPNLT

Sequences:

>Translated_382_residues
MCDKKPLKESSMSHENLKGAQMAEGIPETMRAVVAYGPRDYRFERVPVPTIDAKEILVKVEGCGICAGDTKAFEGAPSFW
GDDKQPAYIKAPMIPGHEFIGHVVGLGADVEGFELGDRVTSEQIVPCWGCRFCNHGQYWMCEKHDLYGFQSNVNGAMAEY
LKFTKEAINYHVPDHLPLEKAILIEPYACAFHAVQRAKIKLGDVVVLAGSGTLGLGMIGAIKKSGAAKLIVLDLSDDRLA
LAKKFGADLVLNPSRDDVNTLIKEMTGGYGCDIYIDATGAQKAVEQGLTLLRKLGTFVEFSVFKDPVTVDWSIISDRKEL
DVLGSHLGPYCFPLVIEGISNGDLPTEGVVTHILPLEKYAEGIELVKRGVDSLKVVLNPNLT
>Mature_382_residues
MCDKKPLKESSMSHENLKGAQMAEGIPETMRAVVAYGPRDYRFERVPVPTIDAKEILVKVEGCGICAGDTKAFEGAPSFW
GDDKQPAYIKAPMIPGHEFIGHVVGLGADVEGFELGDRVTSEQIVPCWGCRFCNHGQYWMCEKHDLYGFQSNVNGAMAEY
LKFTKEAINYHVPDHLPLEKAILIEPYACAFHAVQRAKIKLGDVVVLAGSGTLGLGMIGAIKKSGAAKLIVLDLSDDRLA
LAKKFGADLVLNPSRDDVNTLIKEMTGGYGCDIYIDATGAQKAVEQGLTLLRKLGTFVEFSVFKDPVTVDWSIISDRKEL
DVLGSHLGPYCFPLVIEGISNGDLPTEGVVTHILPLEKYAEGIELVKRGVDSLKVVLNPNLT

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=359, Percent_Identity=30.3621169916435, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=26.8292682926829, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI4501933, Length=371, Percent_Identity=25.3369272237197, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI34577061, Length=372, Percent_Identity=26.8817204301075, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI262073058, Length=392, Percent_Identity=24.7448979591837, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI71743840, Length=385, Percent_Identity=24.9350649350649, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI4501939, Length=374, Percent_Identity=24.5989304812834, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI156523966, Length=374, Percent_Identity=24.5989304812834, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788073, Length=348, Percent_Identity=30.4597701149425, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1790045, Length=336, Percent_Identity=26.7857142857143, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI226510992, Length=341, Percent_Identity=30.791788856305, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787863, Length=340, Percent_Identity=27.3529411764706, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1786825, Length=405, Percent_Identity=27.4074074074074, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788407, Length=364, Percent_Identity=25.2747252747253, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788075, Length=353, Percent_Identity=24.6458923512748, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790718, Length=327, Percent_Identity=25.9938837920489, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI87082125, Length=365, Percent_Identity=26.3013698630137, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081918, Length=219, Percent_Identity=32.8767123287671, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562876, Length=351, Percent_Identity=32.7635327635328, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17562878, Length=351, Percent_Identity=30.4843304843305, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17562584, Length=320, Percent_Identity=27.8125, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17562582, Length=319, Percent_Identity=27.8996865203762, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71988145, Length=291, Percent_Identity=25.085910652921, Blast_Score=81, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323099, Length=345, Percent_Identity=28.4057971014493, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6319257, Length=390, Percent_Identity=26.9230769230769, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319258, Length=380, Percent_Identity=27.3684210526316, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6322619, Length=356, Percent_Identity=25.561797752809, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6319955, Length=356, Percent_Identity=25.561797752809, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323729, Length=330, Percent_Identity=25.7575757575758, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=283, Percent_Identity=26.8551236749117, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320033, Length=394, Percent_Identity=25.3807106598985, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319621, Length=286, Percent_Identity=30.0699300699301, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=283, Percent_Identity=26.5017667844523, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319520, Length=265, Percent_Identity=25.6603773584906, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17137530, Length=352, Percent_Identity=28.9772727272727, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17737897, Length=351, Percent_Identity=29.3447293447293, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI17737895, Length=390, Percent_Identity=25.1282051282051, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 41613; Mature: 41613

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCDKKPLKESSMSHENLKGAQMAEGIPETMRAVVAYGPRDYRFERVPVPTIDAKEILVKV
CCCCCCCCHHCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHEEEEE
EGCGICAGDTKAFEGAPSFWGDDKQPAYIKAPMIPGHEFIGHVVGLGADVEGFELGDRVT
CCCEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
SEQIVPCWGCRFCNHGQYWMCEKHDLYGFQSNVNGAMAEYLKFTKEAINYHVPDHLPLEK
CCCCCCCCCCEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
AILIEPYACAFHAVQRAKIKLGDVVVLAGSGTLGLGMIGAIKKSGAAKLIVLDLSDDRLA
EEEECCHHHHHHHHHHHEEEECCEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCHHH
LAKKFGADLVLNPSRDDVNTLIKEMTGGYGCDIYIDATGAQKAVEQGLTLLRKLGTFVEF
HHHHCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHEE
SVFKDPVTVDWSIISDRKELDVLGSHLGPYCFPLVIEGISNGDLPTEGVVTHILPLEKYA
EEECCCEEEEEEEECCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
EGIELVKRGVDSLKVVLNPNLT
HHHHHHHCCCCCEEEEECCCCC
>Mature Secondary Structure
MCDKKPLKESSMSHENLKGAQMAEGIPETMRAVVAYGPRDYRFERVPVPTIDAKEILVKV
CCCCCCCCHHCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHEEEEE
EGCGICAGDTKAFEGAPSFWGDDKQPAYIKAPMIPGHEFIGHVVGLGADVEGFELGDRVT
CCCEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
SEQIVPCWGCRFCNHGQYWMCEKHDLYGFQSNVNGAMAEYLKFTKEAINYHVPDHLPLEK
CCCCCCCCCCEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
AILIEPYACAFHAVQRAKIKLGDVVVLAGSGTLGLGMIGAIKKSGAAKLIVLDLSDDRLA
EEEECCHHHHHHHHHHHEEEECCEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCHHH
LAKKFGADLVLNPSRDDVNTLIKEMTGGYGCDIYIDATGAQKAVEQGLTLLRKLGTFVEF
HHHHCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHEE
SVFKDPVTVDWSIISDRKELDVLGSHLGPYCFPLVIEGISNGDLPTEGVVTHILPLEKYA
EEECCCEEEEEEEECCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
EGIELVKRGVDSLKVVLNPNLT
HHHHHHHCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]