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The map label for this gene is ispF [H]

Identifier: 22124740

GI number: 22124740

Start: 929250

End: 929747

Strand: Direct

Name: ispF [H]

Synonym: y0829

Alternate gene names: 22124740

Gene position: 929250-929747 (Clockwise)

Preceding gene: 22124739

Following gene: 22124741

Centisome position: 20.2

GC content: 47.79

Gene sequence:

>498_bases
ATGCATATAATGCGGATCGGTCATGGTTTCGATGTTCATAAATTTGGCGAGAATGGCAGTGGTCCACTCATTATTGGTGG
CGTGCGTATCCCATATGAAAAGGGGTTACTGGCCCATTCTGACGGCGATGTTGCATTACATGCGGCCACTGATGCGCTAT
TAGGCGCTGCTGCATTAGGTGATATCGGGAAACTCTTCCCCGACACTGACCCGGCATTTAAAGGCGCAGATAGCCGAGGT
TTACTGCGAGAAGCGTATCGCCGTATTCTGGCCAAAGGCTATAAATTGGGGAATCTGGATATTACCATTATTGCCCAGGC
ACCTAAAATGGCACCGCATATTCCACAAATGCGCGTTAATTTAGCAGAAGACCTACAATGTCATATGGATGACATCAACG
TCAAAGCGACCACCACGGAACAGCTTGGTTTTACCGGGCGTGGTGAAGGTATTGCGTGTGAAGCGGTGGTTTTATTAGTT
AACGTAGAACAGGGTTAA

Upstream 100 bases:

>100_bases
GCTAAACAGCGACAAGTCAGTGAGATGGGTAAGCGGAAGTCGTGAACGTTAATCAAGACTAACGGCACTGCAACGTCAAA
TAGTCAGAATATTATAAGGA

Downstream 100 bases:

>100_bases
GGCATAACGAATGGATATGGAAAACCTGACCTGGCTACACGGTAAACCTACGGCGAGCGGTATTTTAAAAGCGAATCCGG
AAGATTTTGTTGTCGTAGAG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MHIMRIGHGFDVHKFGENGSGPLIIGGVRIPYEKGLLAHSDGDVALHAATDALLGAAALGDIGKLFPDTDPAFKGADSRG
LLREAYRRILAKGYKLGNLDITIIAQAPKMAPHIPQMRVNLAEDLQCHMDDINVKATTTEQLGFTGRGEGIACEAVVLLV
NVEQG

Sequences:

>Translated_165_residues
MHIMRIGHGFDVHKFGENGSGPLIIGGVRIPYEKGLLAHSDGDVALHAATDALLGAAALGDIGKLFPDTDPAFKGADSRG
LLREAYRRILAKGYKLGNLDITIIAQAPKMAPHIPQMRVNLAEDLQCHMDDINVKATTTEQLGFTGRGEGIACEAVVLLV
NVEQG
>Mature_165_residues
MHIMRIGHGFDVHKFGENGSGPLIIGGVRIPYEKGLLAHSDGDVALHAATDALLGAAALGDIGKLFPDTDPAFKGADSRG
LLREAYRRILAKGYKLGNLDITIIAQAPKMAPHIPQMRVNLAEDLQCHMDDINVKATTTEQLGFTGRGEGIACEAVVLLV
NVEQG

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=161, Percent_Identity=83.8509316770186, Blast_Score=258, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17564; Mature: 17564

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIMRIGHGFDVHKFGENGSGPLIIGGVRIPYEKGLLAHSDGDVALHAATDALLGAAALG
CEEEEECCCCCEEECCCCCCCCEEEEEEECCHHCCEEEECCCCEEEEEHHHHHHHHHHHH
DIGKLFPDTDPAFKGADSRGLLREAYRRILAKGYKLGNLDITIIAQAPKMAPHIPQMRVN
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCEEEEEEEECCCCCCCCCHHHHC
LAEDLQCHMDDINVKATTTEQLGFTGRGEGIACEAVVLLVNVEQG
CHHHHEEECCCCEEEEEEHHHCCCCCCCCCEEEEEEEEEEEECCC
>Mature Secondary Structure
MHIMRIGHGFDVHKFGENGSGPLIIGGVRIPYEKGLLAHSDGDVALHAATDALLGAAALG
CEEEEECCCCCEEECCCCCCCCEEEEEEECCHHCCEEEECCCCEEEEEHHHHHHHHHHHH
DIGKLFPDTDPAFKGADSRGLLREAYRRILAKGYKLGNLDITIIAQAPKMAPHIPQMRVN
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCEEEEEEEECCCCCCCCCHHHHC
LAEDLQCHMDDINVKATTTEQLGFTGRGEGIACEAVVLLVNVEQG
CHHHHEEECCCCEEEEEEHHHCCCCCCCCCEEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA