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The map label for this gene is mrcB [H]

Identifier: 22124708

GI number: 22124708

Start: 887837

End: 890155

Strand: Direct

Name: mrcB [H]

Synonym: y0795

Alternate gene names: 22124708

Gene position: 887837-890155 (Clockwise)

Preceding gene: 22124707

Following gene: 22124709

Centisome position: 19.3

GC content: 53.51

Gene sequence:

>2319_bases
ATGCCAAGAAAAGTAGCATCACGCCCACCTCGCAAGAAACGCCGTTGGCTCGGGTTGTTGATCAAACTGTTCCTGATTGG
GGCCGTGGCTTTGGCGATCTATGGGGTTTACCTCGATTCGCAAATCCGCAGCCGTATTGATGGCAAAGTCTGGCAATTAC
CGGCTGCCGTGTATGGCCGCATGGTCAATCTTGAGCCGGGTATGTCCTACAGCAAAAAAGAGATGATCGCCTTGCTGGAA
GGGATGCAATACCGTCAGGTCAGTCGCATGACCCGCCCTGGGGAGTTCACGGTTCAGAACGACAGTATCGATATCTTGCG
CCGGCCGTTCGATTTCCCTGATGGTAAAGAAGGCCAAATTCGCGCCCGTTTACTGTTCAAAAATGATCGTCTGGCCCAGA
TTCAAAACCTGGATAATCAGCGTGATTTCGGGCTATTCCGCCTCGATCCCAAGTTGATTACCATGCTGCAATCACCTAAC
GGTGAACAGCGTCTGTTTGTTCCGCGCGCCGGGTTCCCTGATTTGCTGGTGGACACCTTGCTGGCGACTGAAGACCGCCA
TTTCTATGAGCATGATGGCGTCAGCCCGTATTCCATTGCTCGAGCCGTGGTCGCTAACCTGACAGCCGGTAAAGCGGTGC
AGGGGGGCAGTACGCTGACGCAACAGTTAGTGAAAAACCTGTTCCTGACCAATGAACGTTCGCTGGTACGTAAACTTAAC
GAGGCCTATATGGCCCTGTTGATGGATTATCGCTACAGCAAAGATCGGATCTTGGAACTCTATCTGAACGAAGTCTATCT
TGGTCAGAGTGGTGGTGACCAAATCCGTGGCTTCCCGCTGGGTAGCTTGTATTACTTTGGCCGCCCGGTTGATGAATTGA
GTCTCGATCAGCAAGCCATGTTGGTTGGCATGGTGAAAGGGGCCTCACTGTATAACCCGTGGCGTAATCCTAAGCTGGCA
CTTGAGCGGCGTAATTTGGTCCTGCGCTTGTTGCAGAATCAAGGGATTATCGATGCTGAGCTTTACACCATGCTCAGTGC
CCGTCCGCTGGGGGTGCAGCCGAAAGGCGGAGTCATCACACCTCAACCTGCTTTTATGCAGATGGTGCGCCAGGAGCTGC
AACAGAAGTTGGGTAACAAGGTCAATGATCTGTCTGGCGTGAAGATCTTCACCACCTTGGATCCGGTTTCACAAGATGCC
GCAGAAAAAGCCATCGAAGACGGTGTTCCGGCGCTGAGAGCCGCCCGTAATATGAATGACCTGGAAGCGGCGATGGTGGT
GGTTGACCGTTTCAGTGGCGAAGTGCGTGCCATGGTGGGCGGTTCACAGCCACAATTTGCCGGTTTTAACCGTGCGATGC
AGGCCCGCCGTTTGGTGGGGTCACTTGCCAAACCACCAACCTATCTGGCCGCGTTGAGTGAGCCGGATAAGTACCGCCTC
AATACCTGGCTTTCGGACCAACCGCTGTCGCTTAAATTGTCGAACGGTTCATTGTGGCAGCCGAAAAACTATGATCGCCA
GTTCCGTGGTCAGGTCATGTTGATGGATGCGTTGGTGAACTCACTGAACATCCCAACAGTCAATTTGGGGATGTCAGTGG
GCTTAGATCAGATCAGCGCCACACTGCAACGCCTTGGGATCCCGAAATCAGTCATTAATCCGGTCCCAGCCATGTTATTG
GGGGCGATTGACCTGACTCCGGTTGAAGTTGCGCAGGAGTATCAGACAATCGCCAGTGGTGGCAACCGTGCACCGTTGTC
GGCTGTGCGTTCGGTTATTGCTGAGGATGGGACCGTGCTGTATCAGAGCTTCCCACAGGCAGAACGTATGGTGCCTGCAC
AAGCTTCTTACCTGACGCTGTATGCGATGCAGCAAGGGGTTGTCCGTGGTACATCACGCTCTCTCTCGGCGAAGTTCGGC
AAATATAATCTGGCGGCCAAAACCGGGACAACCAACGATCTGCGTGACAGTTGGTTTGCCGGTATTGATGGCAAAGAAGT
GACCATTGCCTGGATTGGGCGCGACAATAACGGCCCGACTAAGCTGACCGGGGCCAGCGGCGCGCTAACCTTGTATCGCC
GCTATCTGGAAAACCAAACGCCGTTGCCGCTGATTCTGCAACCGCCAGAAGGCATTAGCCAAATGAACATTGATTCGGCG
GGGAACTTCGTCTGTGGTGAAGGGAGCGGGATGCGCGTCATTCCTGTTTGGACCGAAAATCCGCAAGCTTTGTGTCAGGG
ATCAACACCCACTCAAGACCCCACAAAACCTAACGATGATGGTGTTGCCGATTGGATCAAAGAGATGTTTGGCCAATAA

Upstream 100 bases:

>100_bases
AGCAGCACCAAAACGTCCACTGCGCCGTCGGCGTGATGAAGATGAATACGAAGAAGATTATGACGAAGAACAAAATGATT
ATGATGACGAGGAGGAACCG

Downstream 100 bases:

>100_bases
AATGTGTGGTCAATAAAATGTGACGGCGGGCGTGGTTTACAGACTGCGCCCGCCAATTTTTTTCTTTGCGCTTCCCTTCT
TCGTTTCCCCCAACAAATCA

Product: penicillin-binding protein 1b

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 772; Mature: 771

Protein sequence:

>772_residues
MPRKVASRPPRKKRRWLGLLIKLFLIGAVALAIYGVYLDSQIRSRIDGKVWQLPAAVYGRMVNLEPGMSYSKKEMIALLE
GMQYRQVSRMTRPGEFTVQNDSIDILRRPFDFPDGKEGQIRARLLFKNDRLAQIQNLDNQRDFGLFRLDPKLITMLQSPN
GEQRLFVPRAGFPDLLVDTLLATEDRHFYEHDGVSPYSIARAVVANLTAGKAVQGGSTLTQQLVKNLFLTNERSLVRKLN
EAYMALLMDYRYSKDRILELYLNEVYLGQSGGDQIRGFPLGSLYYFGRPVDELSLDQQAMLVGMVKGASLYNPWRNPKLA
LERRNLVLRLLQNQGIIDAELYTMLSARPLGVQPKGGVITPQPAFMQMVRQELQQKLGNKVNDLSGVKIFTTLDPVSQDA
AEKAIEDGVPALRAARNMNDLEAAMVVVDRFSGEVRAMVGGSQPQFAGFNRAMQARRLVGSLAKPPTYLAALSEPDKYRL
NTWLSDQPLSLKLSNGSLWQPKNYDRQFRGQVMLMDALVNSLNIPTVNLGMSVGLDQISATLQRLGIPKSVINPVPAMLL
GAIDLTPVEVAQEYQTIASGGNRAPLSAVRSVIAEDGTVLYQSFPQAERMVPAQASYLTLYAMQQGVVRGTSRSLSAKFG
KYNLAAKTGTTNDLRDSWFAGIDGKEVTIAWIGRDNNGPTKLTGASGALTLYRRYLENQTPLPLILQPPEGISQMNIDSA
GNFVCGEGSGMRVIPVWTENPQALCQGSTPTQDPTKPNDDGVADWIKEMFGQ

Sequences:

>Translated_772_residues
MPRKVASRPPRKKRRWLGLLIKLFLIGAVALAIYGVYLDSQIRSRIDGKVWQLPAAVYGRMVNLEPGMSYSKKEMIALLE
GMQYRQVSRMTRPGEFTVQNDSIDILRRPFDFPDGKEGQIRARLLFKNDRLAQIQNLDNQRDFGLFRLDPKLITMLQSPN
GEQRLFVPRAGFPDLLVDTLLATEDRHFYEHDGVSPYSIARAVVANLTAGKAVQGGSTLTQQLVKNLFLTNERSLVRKLN
EAYMALLMDYRYSKDRILELYLNEVYLGQSGGDQIRGFPLGSLYYFGRPVDELSLDQQAMLVGMVKGASLYNPWRNPKLA
LERRNLVLRLLQNQGIIDAELYTMLSARPLGVQPKGGVITPQPAFMQMVRQELQQKLGNKVNDLSGVKIFTTLDPVSQDA
AEKAIEDGVPALRAARNMNDLEAAMVVVDRFSGEVRAMVGGSQPQFAGFNRAMQARRLVGSLAKPPTYLAALSEPDKYRL
NTWLSDQPLSLKLSNGSLWQPKNYDRQFRGQVMLMDALVNSLNIPTVNLGMSVGLDQISATLQRLGIPKSVINPVPAMLL
GAIDLTPVEVAQEYQTIASGGNRAPLSAVRSVIAEDGTVLYQSFPQAERMVPAQASYLTLYAMQQGVVRGTSRSLSAKFG
KYNLAAKTGTTNDLRDSWFAGIDGKEVTIAWIGRDNNGPTKLTGASGALTLYRRYLENQTPLPLILQPPEGISQMNIDSA
GNFVCGEGSGMRVIPVWTENPQALCQGSTPTQDPTKPNDDGVADWIKEMFGQ
>Mature_771_residues
PRKVASRPPRKKRRWLGLLIKLFLIGAVALAIYGVYLDSQIRSRIDGKVWQLPAAVYGRMVNLEPGMSYSKKEMIALLEG
MQYRQVSRMTRPGEFTVQNDSIDILRRPFDFPDGKEGQIRARLLFKNDRLAQIQNLDNQRDFGLFRLDPKLITMLQSPNG
EQRLFVPRAGFPDLLVDTLLATEDRHFYEHDGVSPYSIARAVVANLTAGKAVQGGSTLTQQLVKNLFLTNERSLVRKLNE
AYMALLMDYRYSKDRILELYLNEVYLGQSGGDQIRGFPLGSLYYFGRPVDELSLDQQAMLVGMVKGASLYNPWRNPKLAL
ERRNLVLRLLQNQGIIDAELYTMLSARPLGVQPKGGVITPQPAFMQMVRQELQQKLGNKVNDLSGVKIFTTLDPVSQDAA
EKAIEDGVPALRAARNMNDLEAAMVVVDRFSGEVRAMVGGSQPQFAGFNRAMQARRLVGSLAKPPTYLAALSEPDKYRLN
TWLSDQPLSLKLSNGSLWQPKNYDRQFRGQVMLMDALVNSLNIPTVNLGMSVGLDQISATLQRLGIPKSVINPVPAMLLG
AIDLTPVEVAQEYQTIASGGNRAPLSAVRSVIAEDGTVLYQSFPQAERMVPAQASYLTLYAMQQGVVRGTSRSLSAKFGK
YNLAAKTGTTNDLRDSWFAGIDGKEVTIAWIGRDNNGPTKLTGASGALTLYRRYLENQTPLPLILQPPEGISQMNIDSAG
NFVCGEGSGMRVIPVWTENPQALCQGSTPTQDPTKPNDDGVADWIKEMFGQ

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=780, Percent_Identity=71.2820512820513, Blast_Score=1114, Evalue=0.0,
Organism=Escherichia coli, GI87082258, Length=283, Percent_Identity=34.6289752650177, Blast_Score=162, Evalue=1e-40,
Organism=Escherichia coli, GI1788867, Length=512, Percent_Identity=28.515625, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1789601, Length=127, Percent_Identity=36.2204724409449, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 85582; Mature: 85451

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKVASRPPRKKRRWLGLLIKLFLIGAVALAIYGVYLDSQIRSRIDGKVWQLPAAVYGR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECHHHHCE
MVNLEPGMSYSKKEMIALLEGMQYRQVSRMTRPGEFTVQNDSIDILRRPFDFPDGKEGQI
EEECCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCEEEECCCHHHHHCCCCCCCCCCCCE
RARLLFKNDRLAQIQNLDNQRDFGLFRLDPKLITMLQSPNGEQRLFVPRAGFPDLLVDTL
EEEEEECCCCHHHHHCCCCCCCCCEEEECHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
LATEDRHFYEHDGVSPYSIARAVVANLTAGKAVQGGSTLTQQLVKNLFLTNERSLVRKLN
HHCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
EAYMALLMDYRYSKDRILELYLNEVYLGQSGGDQIRGFPLGSLYYFGRPVDELSLDQQAM
HHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHCCCCCHHHHCCCHHHH
LVGMVKGASLYNPWRNPKLALERRNLVLRLLQNQGIIDAELYTMLSARPLGVQPKGGVIT
HHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCC
PQPAFMQMVRQELQQKLGNKVNDLSGVKIFTTLDPVSQDAAEKAIEDGVPALRAARNMND
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCHH
LEAAMVVVDRFSGEVRAMVGGSQPQFAGFNRAMQARRLVGSLAKPPTYLAALSEPDKYRL
HHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEE
NTWLSDQPLSLKLSNGSLWQPKNYDRQFRGQVMLMDALVNSLNIPTVNLGMSVGLDQISA
ECCCCCCCCEEEECCCCCCCCCCCCHHHCCCEEEHHHHHHHCCCCEEECCHHCCHHHHHH
TLQRLGIPKSVINPVPAMLLGAIDLTPVEVAQEYQTIASGGNRAPLSAVRSVIAEDGTVL
HHHHHCCCHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEE
YQSFPQAERMVPAQASYLTLYAMQQGVVRGTSRSLSAKFGKYNLAAKTGTTNDLRDSWFA
ECCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHC
GIDGKEVTIAWIGRDNNGPTKLTGASGALTLYRRYLENQTPLPLILQPPEGISQMNIDSA
CCCCCEEEEEEEEECCCCCCEEECCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCC
GNFVCGEGSGMRVIPVWTENPQALCQGSTPTQDPTKPNDDGVADWIKEMFGQ
CCEEECCCCCEEEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PRKVASRPPRKKRRWLGLLIKLFLIGAVALAIYGVYLDSQIRSRIDGKVWQLPAAVYGR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECHHHHCE
MVNLEPGMSYSKKEMIALLEGMQYRQVSRMTRPGEFTVQNDSIDILRRPFDFPDGKEGQI
EEECCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCEEEECCCHHHHHCCCCCCCCCCCCE
RARLLFKNDRLAQIQNLDNQRDFGLFRLDPKLITMLQSPNGEQRLFVPRAGFPDLLVDTL
EEEEEECCCCHHHHHCCCCCCCCCEEEECHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
LATEDRHFYEHDGVSPYSIARAVVANLTAGKAVQGGSTLTQQLVKNLFLTNERSLVRKLN
HHCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
EAYMALLMDYRYSKDRILELYLNEVYLGQSGGDQIRGFPLGSLYYFGRPVDELSLDQQAM
HHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHCCCCCHHHHCCCHHHH
LVGMVKGASLYNPWRNPKLALERRNLVLRLLQNQGIIDAELYTMLSARPLGVQPKGGVIT
HHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCC
PQPAFMQMVRQELQQKLGNKVNDLSGVKIFTTLDPVSQDAAEKAIEDGVPALRAARNMND
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCHH
LEAAMVVVDRFSGEVRAMVGGSQPQFAGFNRAMQARRLVGSLAKPPTYLAALSEPDKYRL
HHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEE
NTWLSDQPLSLKLSNGSLWQPKNYDRQFRGQVMLMDALVNSLNIPTVNLGMSVGLDQISA
ECCCCCCCCEEEECCCCCCCCCCCCHHHCCCEEEHHHHHHHCCCCEEECCHHCCHHHHHH
TLQRLGIPKSVINPVPAMLLGAIDLTPVEVAQEYQTIASGGNRAPLSAVRSVIAEDGTVL
HHHHHCCCHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEE
YQSFPQAERMVPAQASYLTLYAMQQGVVRGTSRSLSAKFGKYNLAAKTGTTNDLRDSWFA
ECCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHC
GIDGKEVTIAWIGRDNNGPTKLTGASGALTLYRRYLENQTPLPLILQPPEGISQMNIDSA
CCCCCEEEEEEEEECCCCCCEEECCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCC
GNFVCGEGSGMRVIPVWTENPQALCQGSTPTQDPTKPNDDGVADWIKEMFGQ
CCEEECCCCCEEEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3882429; 8202364; 9278503; 3920658; 8645198; 9244263; 3330753; 1885547; 10037771; 10564478; 9841666 [H]