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The map label for this gene is amiB [H]

Identifier: 22124541

GI number: 22124541

Start: 708418

End: 710331

Strand: Direct

Name: amiB [H]

Synonym: y0627

Alternate gene names: 22124541

Gene position: 708418-710331 (Clockwise)

Preceding gene: 22124540

Following gene: 22124542

Centisome position: 15.4

GC content: 50.94

Gene sequence:

>1914_bases
ATGATGCATAGCCTCTCGCTGATATGTCGCTGGAAACACGGTTTCGCTACGATTCGCCATTTCACTACGATTTGCCATTT
CACTACTGTCCACCGCGTCATTACTGCTCGTCGTTTCGCCACGGTCACGCGGATGATTATTGGTTTATTAACGCTCAGTT
TATTCGCTCTGCCTCCCGCATTTGCCATGAAACTCACTGACATTAAGGTGACCAACGGGGCGAGTGAAAGTAAGGTTACT
CTCAGCTTTGATGGTAAGCCAATTTATGCATTTTTCTCGCTAAACTCGCCGGAGCGGGTGGTCTTGGATGTCCGCCAAAG
TGGCAATATCTCAGGGTTGCCGCTGGAGTTCAGTGGGCAGAACCTGTTGAAACGAATTCGCTCCAGCACTCCGAAAGATG
AACAAAGTACCCGCCTGGTACTGGAACTGACGCAGAAAGTAAAAACCCGTGCTGTCACCCAACAGTCGGGCAATAACTAC
ACGGTAGTGCTTACCATGACGGCCGTCGCCAGTGCCCCTGTTCGTCAGGCGCAGGCCTCATTAAATCAGACGAATACCCC
GAGTCCTAATGCCGGGCGTGTGGTGCCTCAAGTCACGAGCAAGGATTCAGTAGCTAAAAATCCGTTTAATAATAAGCCCG
TCGTCGTGGTCAGTAGCGAAAACGTGACCACGAATACTGCCCGTCCGATTAAAACTGTGAGTGCGGCTAACAGTAGCCGT
GTTGTGGTGGCCATAGATGCCGGTCATGGTGGTCAGGACCCAGGCGCTATCGGGCAAAATGGTCTGAAAGAGAAAAATGT
CACGATCTCCATTGCCCGCCGGCTTGAGGCACTGCTCAACCGGGATCCGATGTTTAAACCGGTGCTGACCCGCAATGGTG
ATTACTTTATCTCGGTCATGGGGCGCTCTGATGTTGCACGTAAACAGGGGGCTAATGTCTTGATTTCTATTCATGCTGAT
GCCGCGCCAAATCGCAGTGCGACAGGGGCATCGGTTTGGGTGTTATCAAACCGCCGGGCTAATAGTGAAATGGGGAACTG
GCTGGAGCAACATGAAAAGCAATCAGAACTGCTCGGGGGGGCTGGTGATGTACTGGCAAACACGGCTTCAGACCCTTATT
TGAGTCAGGCGGTTCTGGATTTACAATTTGGTCACTCCCAGAGAGTGGGTTATGACGTCGCGACTAAAGTGCTGCGTGAG
CTACAAACGGTGGGTGATATTCATAAACGCAAGCCGGAGCACGCCAGCCTTGGGGTGTTACGTTCACCGGATATTCCGTC
GCTATTAGTGGAAACCGGCTTTATCAGTAATAGTACGGAGGAACGGCTGCTTGGCAGTAGCGCGTATCAGGAGAAGATTG
CTCAGGCCATTTATAAAGGCTTGCGCAGTTATTTCCTCGCGAATCCGTTACAATCCGACCCAAAGGTCGAAAGCCGTCCG
CTCATTGAAACAGCGGCGGTTGACTCCTCAACACAGCGTTCAGGTATTAGTCAGCCGGAACCTATTGTGAATAATATAAG
CAGTAATGCGCAAAGTGGCCGGTCATCGGCTACTCTGCCTGCAGGTAAGAGCAAAATCCACAACGTGCAGCGTGGGGAGA
CGTTATCGGGTATTGCCAGCCAATACGGCGTCAGCATGGCTGTTTTACGACAAAATAATACGCTGAAAAATGATGTAGTC
TGGGTAGGCCAGCGGTTGAAAGTGCCCGCGTCGGGCAGTACGGTAACGGCAGTCAAGCCTAAAACAGCCGCTGTAGCAAA
AAGTAAACCTATTAAGCATCAGGTGAAACGCGGTGATACGCTGTCAGCCATTGCGGCGAGATACGGTGTATCAATGAGTG
AGATTGAGCGGGTGAATAAAATCAAGTCAGGTAATGTTCAGCTTGGGCAAACGCTCACTATTCCACAATCGTAA

Upstream 100 bases:

>100_bases
ACTTATCAGGCGGCAGGGCGTGAAGCCCGGTTGGTCGCTATTTCCGAGGCAGGCGCTGACGTGTTGCGCCGTTTAGCCGC
TGTGCAAGGGTAACGAAACG

Downstream 100 bases:

>100_bases
GCGCAAGGAATTGTTATGCCGATTCAGATTCTGCCTCCACAGCTTGCCAACCAGATTGCCGCCGGTGAGGTGGTAGAGCG
GCCTGCATCGGTAGTCAAAG

Product: N-acetylmuramoyl-l-alanine amidase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MMHSLSLICRWKHGFATIRHFTTICHFTTVHRVITARRFATVTRMIIGLLTLSLFALPPAFAMKLTDIKVTNGASESKVT
LSFDGKPIYAFFSLNSPERVVLDVRQSGNISGLPLEFSGQNLLKRIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNY
TVVLTMTAVASAPVRQAQASLNQTNTPSPNAGRVVPQVTSKDSVAKNPFNNKPVVVVSSENVTTNTARPIKTVSAANSSR
VVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPMFKPVLTRNGDYFISVMGRSDVARKQGANVLISIHAD
AAPNRSATGASVWVLSNRRANSEMGNWLEQHEKQSELLGGAGDVLANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRE
LQTVGDIHKRKPEHASLGVLRSPDIPSLLVETGFISNSTEERLLGSSAYQEKIAQAIYKGLRSYFLANPLQSDPKVESRP
LIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKSKIHNVQRGETLSGIASQYGVSMAVLRQNNTLKNDVV
WVGQRLKVPASGSTVTAVKPKTAAVAKSKPIKHQVKRGDTLSAIAARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS

Sequences:

>Translated_637_residues
MMHSLSLICRWKHGFATIRHFTTICHFTTVHRVITARRFATVTRMIIGLLTLSLFALPPAFAMKLTDIKVTNGASESKVT
LSFDGKPIYAFFSLNSPERVVLDVRQSGNISGLPLEFSGQNLLKRIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNY
TVVLTMTAVASAPVRQAQASLNQTNTPSPNAGRVVPQVTSKDSVAKNPFNNKPVVVVSSENVTTNTARPIKTVSAANSSR
VVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPMFKPVLTRNGDYFISVMGRSDVARKQGANVLISIHAD
AAPNRSATGASVWVLSNRRANSEMGNWLEQHEKQSELLGGAGDVLANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRE
LQTVGDIHKRKPEHASLGVLRSPDIPSLLVETGFISNSTEERLLGSSAYQEKIAQAIYKGLRSYFLANPLQSDPKVESRP
LIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKSKIHNVQRGETLSGIASQYGVSMAVLRQNNTLKNDVV
WVGQRLKVPASGSTVTAVKPKTAAVAKSKPIKHQVKRGDTLSAIAARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS
>Mature_637_residues
MMHSLSLICRWKHGFATIRHFTTICHFTTVHRVITARRFATVTRMIIGLLTLSLFALPPAFAMKLTDIKVTNGASESKVT
LSFDGKPIYAFFSLNSPERVVLDVRQSGNISGLPLEFSGQNLLKRIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNY
TVVLTMTAVASAPVRQAQASLNQTNTPSPNAGRVVPQVTSKDSVAKNPFNNKPVVVVSSENVTTNTARPIKTVSAANSSR
VVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPMFKPVLTRNGDYFISVMGRSDVARKQGANVLISIHAD
AAPNRSATGASVWVLSNRRANSEMGNWLEQHEKQSELLGGAGDVLANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRE
LQTVGDIHKRKPEHASLGVLRSPDIPSLLVETGFISNSTEERLLGSSAYQEKIAQAIYKGLRSYFLANPLQSDPKVESRP
LIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKSKIHNVQRGETLSGIASQYGVSMAVLRQNNTLKNDVV
WVGQRLKVPASGSTVTAVKPKTAAVAKSKPIKHQVKRGDTLSAIAARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=430, Percent_Identity=63.2558139534884, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI87082163, Length=432, Percent_Identity=31.0185185185185, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1788776, Length=224, Percent_Identity=42.8571428571429, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1786405, Length=111, Percent_Identity=36.9369369369369, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 68701; Mature: 68701

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHSLSLICRWKHGFATIRHFTTICHFTTVHRVITARRFATVTRMIIGLLTLSLFALPPA
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
FAMKLTDIKVTNGASESKVTLSFDGKPIYAFFSLNSPERVVLDVRQSGNISGLPLEFSGQ
HHEEEEEEEEECCCCCCEEEEEECCCEEEEEEECCCCCEEEEEEECCCCCCCCEEEECCH
NLLKRIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNYTVVLTMTAVASAPVRQAQAS
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHCCHHHHHHHH
LNQTNTPSPNAGRVVPQVTSKDSVAKNPFNNKPVVVVSSENVTTNTARPIKTVSAANSSR
HCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHEEECCCCCE
VVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPMFKPVLTRNGDYFISVM
EEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEE
GRSDVARKQGANVLISIHADAAPNRSATGASVWVLSNRRANSEMGNWLEQHEKQSELLGG
CCCHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCC
AGDVLANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRELQTVGDIHKRKPEHASLGVL
CCHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
RSPDIPSLLVETGFISNSTEERLLGSSAYQEKIAQAIYKGLRSYFLANPLQSDPKVESRP
CCCCCCHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKSKIHNVQRGETLSGIAS
CCHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHH
QYGVSMAVLRQNNTLKNDVVWVGQRLKVPASGSTVTAVKPKTAAVAKSKPIKHQVKRGDT
HHCCEEEEEECCCCCCCCCEEECCEEECCCCCCEEEEECCCHHHHHCCCCHHHHHHCCCH
LSAIAARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS
HHHHHHHHCCCHHHHHHHHHHCCCCEEECEEECCCCC
>Mature Secondary Structure
MMHSLSLICRWKHGFATIRHFTTICHFTTVHRVITARRFATVTRMIIGLLTLSLFALPPA
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
FAMKLTDIKVTNGASESKVTLSFDGKPIYAFFSLNSPERVVLDVRQSGNISGLPLEFSGQ
HHEEEEEEEEECCCCCCEEEEEECCCEEEEEEECCCCCEEEEEEECCCCCCCCEEEECCH
NLLKRIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNYTVVLTMTAVASAPVRQAQAS
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHCCHHHHHHHH
LNQTNTPSPNAGRVVPQVTSKDSVAKNPFNNKPVVVVSSENVTTNTARPIKTVSAANSSR
HCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHEEECCCCCE
VVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPMFKPVLTRNGDYFISVM
EEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEE
GRSDVARKQGANVLISIHADAAPNRSATGASVWVLSNRRANSEMGNWLEQHEKQSELLGG
CCCHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCC
AGDVLANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRELQTVGDIHKRKPEHASLGVL
CCHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
RSPDIPSLLVETGFISNSTEERLLGSSAYQEKIAQAIYKGLRSYFLANPLQSDPKVESRP
CCCCCCHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKSKIHNVQRGETLSGIAS
CCHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHH
QYGVSMAVLRQNNTLKNDVVWVGQRLKVPASGSTVTAVKPKTAAVAKSKPIKHQVKRGDT
HHCCEEEEEECCCCCCCCCEEECCEEECCCCCCEEEEECCCHHHHHCCCCHHHHHHCCCH
LSAIAARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS
HHHHHHHHCCCHHHHHHHHHHCCCCEEECEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511774; 7610040; 9278503; 1594459 [H]