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The map label for this gene is metE [H]

Identifier: 22124357

GI number: 22124357

Start: 491355

End: 493646

Strand: Direct

Name: metE [H]

Synonym: y0442

Alternate gene names: 22124357

Gene position: 491355-493646 (Clockwise)

Preceding gene: 22124355

Following gene: 161484894

Centisome position: 10.68

GC content: 49.78

Gene sequence:

>2292_bases
ATGGGGAATAAGACGATGACAATTTTAAATCACACACTGGGTTTTCCGCGTGTTGGTCTGAAACGTGAACTGAAGAAAGC
ACAAGAAAGTTACTGGGCAGGCAACTCAACACAGGAAGAATTGCTCAATGTGGGGCGTGAATTGCGTGCTCGCCATTGGC
AACAGCAACAACAAGCGGGTGTTGATTTAGTGCCAGTAGGTGATTTCGCTTGGTATGACCATGTCCTGACAACCAGCCTG
TTGCTGGGTAACGTTCCAGAGCGCCACCAAAATGCTGATGGCTCGATTGATATAGACACGTTGTTCCGTATTGGTCGTGG
CCGTGCTCCAACCGGTAAGCCCGCAGCGGCTGCCGAGATGACTAAATGGTTTAATACCAACTATCACTACATGGTGCCTG
AGTTCCAGCAAGGCCAGCAGTTTAAACTGGGTTGGACTCAACTGCTGGATGAAGTGGACGAAGCGCTGGCCTTGGGCCAC
AAAATTAAACCTGTGCTGCTTGGCCCGATCACTTATCTTTGGTTGGGTAAAGTCAAAGGTGAGCAGTTCGATCGCCTCTC
GCTACTGAACGATATTTTGCCGGTTTACCAGCAGGTGTTGGCTGAATTAGCGAAGCGGGGAATCGAGTGGGTGCAGATTG
ATGAGCCAGCATTGGTTCTGGAATTACCACAAGAGTGGTTGGATGCCTATCAACCGGCTTATCAGGCCCTGCAAGGTCAG
GTCAAATTATTGCTGACAACTTACTTCGACAGTATCGGCCATAACATCGACACTATCCGCGCTCTGCCAGTGCAAGGCTT
GCATGTTGATGTGGTTACGGGTCATGACGATCTTGCTGTTTTGAATAAAAATTTACCAAAAGAGTGGTTGTTATCTCTGG
GGGTCATTAATGGCCGTAACGTATGGCGTGCTGACCTGAGTAGTTGGTTTGAACGTCTGCAGCCATTGGTTAATAGCCGC
CCGCTGTGGTTGGGTAGCTCATGCTCCTTATTGCACAGCCCGATTGATTTAAATGAAGAGACCCGTCTTGATGCAGAAGT
GAAGAGCTGGTTTGCCTTCGCCCTGCAAAAATGTGCTGAGTTAGCGCTGCTAACACAAGCATTGAATGCACCTAATGACG
CTAAGCTGGCAGAACTGGCTGCGTACAGTGCGCCGATCCGCGCCCGTCGTTCCTCCAGCCGCGTGCATAATGCGCAAGTT
GAGCAGCGTTTAGCTGCGATTACTTCGCAGGATATTGAGCGCCAACTGCCTTATGAAGCACGTGCTGAAACTCAGCGTAA
GCGTTTTAATTTGCCTGCCTGGCCAACCACGACCATTGGTTCTTTCCCACAAACGACAGAGATCCGTGGTTTACGCCTTG
ATTTTAAACAAGGTCGTCTGGACGGTAAAAACTATCGCACAGGGATCAGCGAGCATATCAAACACGCTATTGCTGAACAG
GAACGCTTGGGCCTGGACGTGCTGGTTCACGGTGAAGCAGAACGTAACGACATGGTCGAATACTTCGGCGAACACTTGGA
TGGTTTTGTCTTTACACAAAATGGTTGGGTACAGAGCTACGGTTCCCGCTGTGTGAAACCGCCGGTCATCATTGGTGATA
TTAGCCGCCCAGAAGCGATTACTGTTGAGTGGGCGAAATATGCTCAATCCTTAACAGAGAAACCGGTAAAAGGCATGCTT
ACCGGCCCGGTCACTATTCTGTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAGACGATTGCCAAACAAATTGCGCT
GGCTTTGCGTGACGAAGTGGAAGATTTAGAAAAAGCAGGCATTGGTATCATCCAGATTGATGAACCTGCATTACGTGAAG
GTTTGCCTCTGCGCCGTGCTGACTGGCAAGCCTATCTGCAATGGGCCGTCGACGCCTTTAAATTGAATGCCGCTGTGGCG
CAAAACGATACACAGATCCATACCCACATGTGTTATTGCGAGTTCAATGACATCATGGATTCCATTGCCGCACTGGATGC
TGATGTGATTACCATCGAAACCTCCCGCTCAGATATGGAACTGTTGGAATCATTTGAAGACTTCGCTTATCCGAATGAAA
TTGGTCCCGGTGTTTATGATATTCATTCACCGAATGTGCCAAGTGTTGAATGGATTGAAGCGTTATTACGCAAAGCCGCT
CAACGTATTCCGGCCGAGCGTTTGTGGGTGAACCCAGATTGTGGTTTGAAAACCCGCGGCTGGCCTGAGACGCGTCAGGC
TCTGGCGAATATGGTGCTGGCAGCGCAGCGTCTGCGTGAAGAACAGGTTTAA

Upstream 100 bases:

>100_bases
GTTCGATCATGAGAGTCCTTCACAGTGACTGTGAAAAAAATGCGCTTGTGGAATATACAGTACCTGCGGATTATAGATGT
GTAAACATCTGGATGGCTAA

Downstream 100 bases:

>100_bases
TTAAACCGTTTTACTCTGTGTAGTGGGGCTTTTGGGGCGCTTTCGCGCCCTTTTTTATTGCGTTTCTTCTGGAATGGGGA
TGGCCGAGACACCATGTTGG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 763; Mature: 762

Protein sequence:

>763_residues
MGNKTMTILNHTLGFPRVGLKRELKKAQESYWAGNSTQEELLNVGRELRARHWQQQQQAGVDLVPVGDFAWYDHVLTTSL
LLGNVPERHQNADGSIDIDTLFRIGRGRAPTGKPAAAAEMTKWFNTNYHYMVPEFQQGQQFKLGWTQLLDEVDEALALGH
KIKPVLLGPITYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQEWLDAYQPAYQALQGQ
VKLLLTTYFDSIGHNIDTIRALPVQGLHVDVVTGHDDLAVLNKNLPKEWLLSLGVINGRNVWRADLSSWFERLQPLVNSR
PLWLGSSCSLLHSPIDLNEETRLDAEVKSWFAFALQKCAELALLTQALNAPNDAKLAELAAYSAPIRARRSSSRVHNAQV
EQRLAAITSQDIERQLPYEARAETQRKRFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGKNYRTGISEHIKHAIAEQ
ERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWAKYAQSLTEKPVKGML
TGPVTILCWSFPREDVSRETIAKQIALALRDEVEDLEKAGIGIIQIDEPALREGLPLRRADWQAYLQWAVDAFKLNAAVA
QNDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEDFAYPNEIGPGVYDIHSPNVPSVEWIEALLRKAA
QRIPAERLWVNPDCGLKTRGWPETRQALANMVLAAQRLREEQV

Sequences:

>Translated_763_residues
MGNKTMTILNHTLGFPRVGLKRELKKAQESYWAGNSTQEELLNVGRELRARHWQQQQQAGVDLVPVGDFAWYDHVLTTSL
LLGNVPERHQNADGSIDIDTLFRIGRGRAPTGKPAAAAEMTKWFNTNYHYMVPEFQQGQQFKLGWTQLLDEVDEALALGH
KIKPVLLGPITYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQEWLDAYQPAYQALQGQ
VKLLLTTYFDSIGHNIDTIRALPVQGLHVDVVTGHDDLAVLNKNLPKEWLLSLGVINGRNVWRADLSSWFERLQPLVNSR
PLWLGSSCSLLHSPIDLNEETRLDAEVKSWFAFALQKCAELALLTQALNAPNDAKLAELAAYSAPIRARRSSSRVHNAQV
EQRLAAITSQDIERQLPYEARAETQRKRFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGKNYRTGISEHIKHAIAEQ
ERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWAKYAQSLTEKPVKGML
TGPVTILCWSFPREDVSRETIAKQIALALRDEVEDLEKAGIGIIQIDEPALREGLPLRRADWQAYLQWAVDAFKLNAAVA
QNDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEDFAYPNEIGPGVYDIHSPNVPSVEWIEALLRKAA
QRIPAERLWVNPDCGLKTRGWPETRQALANMVLAAQRLREEQV
>Mature_762_residues
GNKTMTILNHTLGFPRVGLKRELKKAQESYWAGNSTQEELLNVGRELRARHWQQQQQAGVDLVPVGDFAWYDHVLTTSLL
LGNVPERHQNADGSIDIDTLFRIGRGRAPTGKPAAAAEMTKWFNTNYHYMVPEFQQGQQFKLGWTQLLDEVDEALALGHK
IKPVLLGPITYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQEWLDAYQPAYQALQGQV
KLLLTTYFDSIGHNIDTIRALPVQGLHVDVVTGHDDLAVLNKNLPKEWLLSLGVINGRNVWRADLSSWFERLQPLVNSRP
LWLGSSCSLLHSPIDLNEETRLDAEVKSWFAFALQKCAELALLTQALNAPNDAKLAELAAYSAPIRARRSSSRVHNAQVE
QRLAAITSQDIERQLPYEARAETQRKRFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGKNYRTGISEHIKHAIAEQE
RLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWAKYAQSLTEKPVKGMLT
GPVTILCWSFPREDVSRETIAKQIALALRDEVEDLEKAGIGIIQIDEPALREGLPLRRADWQAYLQWAVDAFKLNAAVAQ
NDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEDFAYPNEIGPGVYDIHSPNVPSVEWIEALLRKAAQ
RIPAERLWVNPDCGLKTRGWPETRQALANMVLAAQRLREEQV

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=754, Percent_Identity=85.0132625994695, Blast_Score=1331, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=769, Percent_Identity=47.9843953185956, Blast_Score=709, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 86210; Mature: 86079

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNKTMTILNHTLGFPRVGLKRELKKAQESYWAGNSTQEELLNVGRELRARHWQQQQQAG
CCCCEEEHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
VDLVPVGDFAWYDHVLTTSLLLGNVPERHQNADGSIDIDTLFRIGRGRAPTGKPAAAAEM
CEEEECCCHHHHHHHHHHHHHHCCCCHHHCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHH
TKWFNTNYHYMVPEFQQGQQFKLGWTQLLDEVDEALALGHKIKPVLLGPITYLWLGKVKG
HHHHCCCCEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCC
EQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQEWLDAYQPAYQALQGQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHH
VKLLLTTYFDSIGHNIDTIRALPVQGLHVDVVTGHDDLAVLNKNLPKEWLLSLGVINGRN
HHHHHHHHHHHHCCCCHHEEECCCCCEEEEEEECCCCHHHHHCCCCHHHHHHHCCCCCCC
VWRADLSSWFERLQPLVNSRPLWLGSSCSLLHSPIDLNEETRLDAEVKSWFAFALQKCAE
HHHHHHHHHHHHHHHHHCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LALLTQALNAPNDAKLAELAAYSAPIRARRSSSRVHNAQVEQRLAAITSQDIERQLPYEA
HHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RAETQRKRFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGKNYRTGISEHIKHAIAEQ
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCHHHCCHHHHHHHHHHHH
ERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAI
HHCCCEEEEECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCEE
TVEWAKYAQSLTEKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVEDLEKAG
EEHHHHHHHHHHHCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IGIIQIDEPALREGLPLRRADWQAYLQWAVDAFKLNAAVAQNDTQIHTHMCYCEFNDIMD
CCEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHH
SIAALDADVITIETSRSDMELLESFEDFAYPNEIGPGVYDIHSPNVPSVEWIEALLRKAA
HHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
QRIPAERLWVNPDCGLKTRGWPETRQALANMVLAAQRLREEQV
HHCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GNKTMTILNHTLGFPRVGLKRELKKAQESYWAGNSTQEELLNVGRELRARHWQQQQQAG
CCCEEEHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
VDLVPVGDFAWYDHVLTTSLLLGNVPERHQNADGSIDIDTLFRIGRGRAPTGKPAAAAEM
CEEEECCCHHHHHHHHHHHHHHCCCCHHHCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHH
TKWFNTNYHYMVPEFQQGQQFKLGWTQLLDEVDEALALGHKIKPVLLGPITYLWLGKVKG
HHHHCCCCEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCC
EQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQEWLDAYQPAYQALQGQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHH
VKLLLTTYFDSIGHNIDTIRALPVQGLHVDVVTGHDDLAVLNKNLPKEWLLSLGVINGRN
HHHHHHHHHHHHCCCCHHEEECCCCCEEEEEEECCCCHHHHHCCCCHHHHHHHCCCCCCC
VWRADLSSWFERLQPLVNSRPLWLGSSCSLLHSPIDLNEETRLDAEVKSWFAFALQKCAE
HHHHHHHHHHHHHHHHHCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LALLTQALNAPNDAKLAELAAYSAPIRARRSSSRVHNAQVEQRLAAITSQDIERQLPYEA
HHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RAETQRKRFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGKNYRTGISEHIKHAIAEQ
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCHHHCCHHHHHHHHHHHH
ERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAI
HHCCCEEEEECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCEE
TVEWAKYAQSLTEKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVEDLEKAG
EEHHHHHHHHHHHCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IGIIQIDEPALREGLPLRRADWQAYLQWAVDAFKLNAAVAQNDTQIHTHMCYCEFNDIMD
CCEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHH
SIAALDADVITIETSRSDMELLESFEDFAYPNEIGPGVYDIHSPNVPSVEWIEALLRKAA
HHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
QRIPAERLWVNPDCGLKTRGWPETRQALANMVLAAQRLREEQV
HHCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA