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The map label for this gene is yifB [H]

Identifier: 22124251

GI number: 22124251

Start: 368467

End: 370011

Strand: Reverse

Name: yifB [H]

Synonym: y0334

Alternate gene names: 22124251

Gene position: 370011-368467 (Counterclockwise)

Preceding gene: 22124257

Following gene: 22124249

Centisome position: 8.04

GC content: 53.27

Gene sequence:

>1545_bases
ATGACGGCAAAGGAAAGCATAATGTCACTGGCAACCATTCATACTCGCGCAACATTGGGCATTCAAGCCCCTCCTGTCGC
GGTAGAAGTTCATATCAGCAATGGATTACCCGGATTGATATTGGTCGGCTTACCTGAAACAACGGTCAAAGAAGCACGGG
ATCGGGTTCGTAGCGCATTAATTAACAGCGGTTTTAATTTTCCCGCCAAGCGGATCACCGTCAGCCTGGCACCGGCAGAT
TTACCCAAAGAAGGCGGGCGCTATGATCTCCCCATTGCTTTAGCCATCTTGGCCGCCTCAGAGCAGATCCCTGCGGATAA
GTTAGCGCACTATGAGTTCTTGGGTGAATTAGCCCTATCCGGCGCGTTAAGGCGCGTTAGCGGCGCAATTCCAGCAGCAC
TCACCTGTAGCGAAGCTAACCGTCAGTTAATCCTTCCCACCGCTAATGGTCTTGAAATTGGTCTAATCCCACAAGGCAAC
TCCTGGGTCGCAGACCATTTATTAGCGGTATGCGGTTTCTTACAAGGTGAAAACCCCCTCGCACAGGGCCAGCCCTTCGA
ACCGGCCCCTTCACCGGATAACCACCTTGATTTACACGACATCATTGGTCAATCGCAGGCTAAACGTGCGTTAGAAATCG
CGGCGGCAGGTGGTCATAACCTGCTGTTACTCGGGCCACCAGGTACCGGTAAAACGATGCTGGCAACCCGCCTAACCGGG
TTATTGCCTCCGCTGACCGATCAGGAGGCGTTAGAAGCAGCGGCGATCACCGGGTTGCTGCATAGCAACGCACTGCCAAC
ACAATGGCGTTGCCGGGCCTTTCGTGCCCCACACCATAGCGCATCGATGGCAGCGCTGATTGGCGGCGGCTCCATCCCCC
GCCCCGGTGAGATATCACTGGCCCATAACGGTGTGTTATTTCTCGATGAACTGCCTGAATTTGAACGCCGGGTACTGGAT
TCACTGCGCGAACCGTTGGAATCTGGCGAAATTATTATCTCCCGCGCGGCAGCCAAAATCTGTTTTCCAGCCAAAGTGCA
ACTTATCGCGGCGATGAACCCTAGCCCCAGCGGCCACTACCAAGGCGTTCATAACCGCACACCGCCACAGCAAATTTTGC
GCTATCTGGCCAAGTTGTCCGGGCCATTTCTCGATAGATTCGATTTATCGATAGAAGTGCCATTACTGCCAGCCGGTATG
CTGGGTGCGCAGAAAAATCAGGGGGAGAGTAGCGCTACAGTCAAGCAGCGAGTGTTACAGGCCAGACAAAGGCAAATGGA
CCGGGCGGGGAAGATCAATACGCAGCTCACTAGCCAAGAGGTAGCAGAGTTTTGTTATTTAGCACCTGAGGATGCCGCGT
TTCTCGAGCAGGTTTTACTGACACTCGGCCTGTCAGTACGTGCCTGGCACCATATTTTGAAAGTGGCGCGAACCATTGCC
GATTTAGCGCAAGAAAAGACCATCCAAAAAAGTCATCTTTCAGAGGCGCTGAGCTATCGCTGTATGGATCGCCTGCTATT
GCAGTTGCACAAAAGCCTGATGTAA

Upstream 100 bases:

>100_bases
AAGGGAAGCATGCGGCACCCTATTTAGGTTCTCATTTATCGCCACACTGAGCACATCAAAATAAAAACATCCCACGGAGC
AGTATCGTGCGTCAGGACGC

Downstream 100 bases:

>100_bases
CAAAGGGGGCCAAAGCCCCCTAATACTCAACTAACCACCGATAGGTAAACGAATCCCGATGAGCTTACACCAGTAAGTGA
TTCGGGTGAGTGCACGCAGC

Product: two-component regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTAKESIMSLATIHTRATLGIQAPPVAVEVHISNGLPGLILVGLPETTVKEARDRVRSALINSGFNFPAKRITVSLAPAD
LPKEGGRYDLPIALAILAASEQIPADKLAHYEFLGELALSGALRRVSGAIPAALTCSEANRQLILPTANGLEIGLIPQGN
SWVADHLLAVCGFLQGENPLAQGQPFEPAPSPDNHLDLHDIIGQSQAKRALEIAAAGGHNLLLLGPPGTGKTMLATRLTG
LLPPLTDQEALEAAAITGLLHSNALPTQWRCRAFRAPHHSASMAALIGGGSIPRPGEISLAHNGVLFLDELPEFERRVLD
SLREPLESGEIIISRAAAKICFPAKVQLIAAMNPSPSGHYQGVHNRTPPQQILRYLAKLSGPFLDRFDLSIEVPLLPAGM
LGAQKNQGESSATVKQRVLQARQRQMDRAGKINTQLTSQEVAEFCYLAPEDAAFLEQVLLTLGLSVRAWHHILKVARTIA
DLAQEKTIQKSHLSEALSYRCMDRLLLQLHKSLM

Sequences:

>Translated_514_residues
MTAKESIMSLATIHTRATLGIQAPPVAVEVHISNGLPGLILVGLPETTVKEARDRVRSALINSGFNFPAKRITVSLAPAD
LPKEGGRYDLPIALAILAASEQIPADKLAHYEFLGELALSGALRRVSGAIPAALTCSEANRQLILPTANGLEIGLIPQGN
SWVADHLLAVCGFLQGENPLAQGQPFEPAPSPDNHLDLHDIIGQSQAKRALEIAAAGGHNLLLLGPPGTGKTMLATRLTG
LLPPLTDQEALEAAAITGLLHSNALPTQWRCRAFRAPHHSASMAALIGGGSIPRPGEISLAHNGVLFLDELPEFERRVLD
SLREPLESGEIIISRAAAKICFPAKVQLIAAMNPSPSGHYQGVHNRTPPQQILRYLAKLSGPFLDRFDLSIEVPLLPAGM
LGAQKNQGESSATVKQRVLQARQRQMDRAGKINTQLTSQEVAEFCYLAPEDAAFLEQVLLTLGLSVRAWHHILKVARTIA
DLAQEKTIQKSHLSEALSYRCMDRLLLQLHKSLM
>Mature_513_residues
TAKESIMSLATIHTRATLGIQAPPVAVEVHISNGLPGLILVGLPETTVKEARDRVRSALINSGFNFPAKRITVSLAPADL
PKEGGRYDLPIALAILAASEQIPADKLAHYEFLGELALSGALRRVSGAIPAALTCSEANRQLILPTANGLEIGLIPQGNS
WVADHLLAVCGFLQGENPLAQGQPFEPAPSPDNHLDLHDIIGQSQAKRALEIAAAGGHNLLLLGPPGTGKTMLATRLTGL
LPPLTDQEALEAAAITGLLHSNALPTQWRCRAFRAPHHSASMAALIGGGSIPRPGEISLAHNGVLFLDELPEFERRVLDS
LREPLESGEIIISRAAAKICFPAKVQLIAAMNPSPSGHYQGVHNRTPPQQILRYLAKLSGPFLDRFDLSIEVPLLPAGML
GAQKNQGESSATVKQRVLQARQRQMDRAGKINTQLTSQEVAEFCYLAPEDAAFLEQVLLTLGLSVRAWHHILKVARTIAD
LAQEKTIQKSHLSEALSYRCMDRLLLQLHKSLM

Specific function: Unknown

COG id: COG0606

COG function: function code O; Predicted ATPase with chaperone activity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Mg-chelatase subunits D/I family. ComM subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693202, Length=506, Percent_Identity=63.4387351778656, Blast_Score=657, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004482
- InterPro:   IPR000523
- InterPro:   IPR020568 [H]

Pfam domain/function: PF01078 Mg_chelatase [H]

EC number: NA

Molecular weight: Translated: 55253; Mature: 55121

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS50051 MCM_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKESIMSLATIHTRATLGIQAPPVAVEVHISNGLPGLILVGLPETTVKEARDRVRSAL
CCCHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHH
INSGFNFPAKRITVSLAPADLPKEGGRYDLPIALAILAASEQIPADKLAHYEFLGELALS
HHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
GALRRVSGAIPAALTCSEANRQLILPTANGLEIGLIPQGNSWVADHLLAVCGFLQGENPL
HHHHHHHCCCCCEEEECCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCC
AQGQPFEPAPSPDNHLDLHDIIGQSQAKRALEIAAAGGHNLLLLGPPGTGKTMLATRLTG
CCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHH
LLPPLTDQEALEAAAITGLLHSNALPTQWRCRAFRAPHHSASMAALIGGGSIPRPGEISL
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHEECCCCCCCCCCEEE
AHNGVLFLDELPEFERRVLDSLREPLESGEIIISRAAAKICFPAKVQLIAAMNPSPSGHY
ECCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCCEEEEEEECCCCCCCCC
QGVHNRTPPQQILRYLAKLSGPFLDRFDLSIEVPLLPAGMLGAQKNQGESSATVKQRVLQ
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHCCCCCCCCHHHHHHHHHHH
ARQRQMDRAGKINTQLTSQEVAEFCYLAPEDAAFLEQVLLTLGLSVRAWHHILKVARTIA
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
DLAQEKTIQKSHLSEALSYRCMDRLLLQLHKSLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAKESIMSLATIHTRATLGIQAPPVAVEVHISNGLPGLILVGLPETTVKEARDRVRSAL
CCHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHH
INSGFNFPAKRITVSLAPADLPKEGGRYDLPIALAILAASEQIPADKLAHYEFLGELALS
HHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
GALRRVSGAIPAALTCSEANRQLILPTANGLEIGLIPQGNSWVADHLLAVCGFLQGENPL
HHHHHHHCCCCCEEEECCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCC
AQGQPFEPAPSPDNHLDLHDIIGQSQAKRALEIAAAGGHNLLLLGPPGTGKTMLATRLTG
CCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHH
LLPPLTDQEALEAAAITGLLHSNALPTQWRCRAFRAPHHSASMAALIGGGSIPRPGEISL
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHEECCCCCCCCCCEEE
AHNGVLFLDELPEFERRVLDSLREPLESGEIIISRAAAKICFPAKVQLIAAMNPSPSGHY
ECCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCCEEEEEEECCCCCCCCC
QGVHNRTPPQQILRYLAKLSGPFLDRFDLSIEVPLLPAGMLGAQKNQGESSATVKQRVLQ
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHCCCCCCCCHHHHHHHHHHH
ARQRQMDRAGKINTQLTSQEVAEFCYLAPEDAAFLEQVLLTLGLSVRAWHHILKVARTIA
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
DLAQEKTIQKSHLSEALSYRCMDRLLLQLHKSLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1995430; 1379743; 9278503 [H]