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The map label for this gene is dusB [H]

Identifier: 22124133

GI number: 22124133

Start: 228754

End: 229821

Strand: Direct

Name: dusB [H]

Synonym: y0213

Alternate gene names: 22124133

Gene position: 228754-229821 (Clockwise)

Preceding gene: 161484913

Following gene: 22124134

Centisome position: 4.97

GC content: 51.03

Gene sequence:

>1068_bases
TTGCTCTTTTTTCATCAATTGGTCAAAGTTTGGCCTTTCATCTGGGCGCAAAAATGCGTAATATACGCGCCCTTACGGAC
ACAGTGTGGTCATTCTTTGTCTATGCGTATTGGACACTTCCAGCTTACAAATTGCCTGATTGCCGCCCCGATGGCGGGTA
TCACAGATCGCCCCTTCAGAGCGCTATGTCATGGTATGGGGGCTGGGATGGCTGTATCTGAAATGCTCTCCTCTAATCCA
GAGGTTTGGCGAACGGATAAGTCGCGTTTACGCATGGTCCATGTTGATGAACCCGGGATTCGTAACGTGCAAATTGCCGG
TAACGATCCAGATGAGATGGCAGCAGCCGCCAGAATCAATGTGGCGAGTGGTGCCCAAATCATCGATATCAATATGGGAT
GTCCAGCCAAGAAAGTGAACCGCAAACTGGCGGGTTCTGCATTATTGCAGCATCCTGATCTGGTCAAACAGATCCTCTCC
GCAGTGGTTAATGCGGTAGATGTGCCGGTGACGTTGAAGATCCGGACGGGTTGGTCACCAGAACACCGTAACTGTATAGA
AATTGCCCAACTGGCCGAAAATTGTGGTATACAAGCCCTGACGATTCATGGCCGAACCCGTTCTTGCCTGTTTAATGGTG
AGGCGGAATACGACAGTATTCGAGCAGTTAAGCAGACTGTTTCCATTCCCGTTATCGCGAATGGTGACATCACTGACCCG
CATAAAGCCAGAGCGGTACTCGACTACACTGGAGCTGATGCTCTGATGATAGGCCGTGCCGCTCAGGGAAGACCGTGGAT
CTTCCGGGAAATCCAGCATTATCTGGACACTGGGGAACTGCTGCCACCGATGCCACTTGGCGAAGTGCAGCGTTTGTTAG
ACGGGCATATACGGGAATTGCACGACTTTTATGGTCCAGGCAAGGGATTTCGTATTGCACGTAAGCACGTCTCTTGGTAT
CTCCAAGAGCACGCCCCTAACGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTT
GGAGGCATATTTCGAAAATCTTGCGTAA

Upstream 100 bases:

>100_bases
TTTGACAGAAAATACTAAAGCGCACAATTCAACCAGATAAGCGTAACGTGATGTTATTTATAATTAAATATCTATAAATA
CAACGTTAGCCTATTATTCA

Downstream 100 bases:

>100_bases
ACAGAAAAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTGCAACCGTAAACTCACA
AGATCAGGTGACTCAAAAGC

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MLFFHQLVKVWPFIWAQKCVIYAPLRTQCGHSLSMRIGHFQLTNCLIAAPMAGITDRPFRALCHGMGAGMAVSEMLSSNP
EVWRTDKSRLRMVHVDEPGIRNVQIAGNDPDEMAAAARINVASGAQIIDINMGCPAKKVNRKLAGSALLQHPDLVKQILS
AVVNAVDVPVTLKIRTGWSPEHRNCIEIAQLAENCGIQALTIHGRTRSCLFNGEAEYDSIRAVKQTVSIPVIANGDITDP
HKARAVLDYTGADALMIGRAAQGRPWIFREIQHYLDTGELLPPMPLGEVQRLLDGHIRELHDFYGPGKGFRIARKHVSWY
LQEHAPNDQFRRTFNAIEDASEQLEALEAYFENLA

Sequences:

>Translated_355_residues
MLFFHQLVKVWPFIWAQKCVIYAPLRTQCGHSLSMRIGHFQLTNCLIAAPMAGITDRPFRALCHGMGAGMAVSEMLSSNP
EVWRTDKSRLRMVHVDEPGIRNVQIAGNDPDEMAAAARINVASGAQIIDINMGCPAKKVNRKLAGSALLQHPDLVKQILS
AVVNAVDVPVTLKIRTGWSPEHRNCIEIAQLAENCGIQALTIHGRTRSCLFNGEAEYDSIRAVKQTVSIPVIANGDITDP
HKARAVLDYTGADALMIGRAAQGRPWIFREIQHYLDTGELLPPMPLGEVQRLLDGHIRELHDFYGPGKGFRIARKHVSWY
LQEHAPNDQFRRTFNAIEDASEQLEALEAYFENLA
>Mature_355_residues
MLFFHQLVKVWPFIWAQKCVIYAPLRTQCGHSLSMRIGHFQLTNCLIAAPMAGITDRPFRALCHGMGAGMAVSEMLSSNP
EVWRTDKSRLRMVHVDEPGIRNVQIAGNDPDEMAAAARINVASGAQIIDINMGCPAKKVNRKLAGSALLQHPDLVKQILS
AVVNAVDVPVTLKIRTGWSPEHRNCIEIAQLAENCGIQALTIHGRTRSCLFNGEAEYDSIRAVKQTVSIPVIANGDITDP
HKARAVLDYTGADALMIGRAAQGRPWIFREIQHYLDTGELLPPMPLGEVQRLLDGHIRELHDFYGPGKGFRIARKHVSWY
LQEHAPNDQFRRTFNAIEDASEQLEALEAYFENLA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=310, Percent_Identity=30.3225806451613, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI239788483, Length=247, Percent_Identity=29.9595141700405, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI239788462, Length=235, Percent_Identity=31.9148936170213, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI8923374, Length=251, Percent_Identity=31.8725099601594, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=31.140350877193, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=87.8504672897196, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1788462, Length=199, Percent_Identity=35.678391959799, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI145693211, Length=246, Percent_Identity=26.0162601626016, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17507177, Length=241, Percent_Identity=30.7053941908714, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=236, Percent_Identity=31.7796610169492, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=244, Percent_Identity=31.5573770491803, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=223, Percent_Identity=32.2869955156951, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=29.4372294372294, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=262, Percent_Identity=30.5343511450382, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=28.9473684210526, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585320, Length=234, Percent_Identity=29.0598290598291, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24580595, Length=231, Percent_Identity=32.9004329004329, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19920448, Length=231, Percent_Identity=32.9004329004329, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45549423, Length=258, Percent_Identity=31.7829457364341, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921524, Length=328, Percent_Identity=25.3048780487805, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 39498; Mature: 39498

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFFHQLVKVWPFIWAQKCVIYAPLRTQCGHSLSMRIGHFQLTNCLIAAPMAGITDRPFR
CHHHHHHHHHHHHHHHCHHEEEECHHHHCCCCHHHEECHHHHHHHHHHCCCCCCCCCHHH
ALCHGMGAGMAVSEMLSSNPEVWRTDKSRLRMVHVDEPGIRNVQIAGNDPDEMAAAARIN
HHHHHCCCCHHHHHHHHCCCCEEECCHHHEEEEECCCCCCCEEEECCCCHHHHHHHHEEE
VASGAQIIDINMGCPAKKVNRKLAGSALLQHPDLVKQILSAVVNAVDVPVTLKIRTGWSP
ECCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCC
EHRNCIEIAQLAENCGIQALTIHGRTRSCLFNGEAEYDSIRAVKQTVSIPVIANGDITDP
CCCHHHHHHHHHHHCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCC
HKARAVLDYTGADALMIGRAAQGRPWIFREIQHYLDTGELLPPMPLGEVQRLLDGHIREL
HHHCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
HDFYGPGKGFRIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENLA
HHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLFFHQLVKVWPFIWAQKCVIYAPLRTQCGHSLSMRIGHFQLTNCLIAAPMAGITDRPFR
CHHHHHHHHHHHHHHHCHHEEEECHHHHCCCCHHHEECHHHHHHHHHHCCCCCCCCCHHH
ALCHGMGAGMAVSEMLSSNPEVWRTDKSRLRMVHVDEPGIRNVQIAGNDPDEMAAAARIN
HHHHHCCCCHHHHHHHHCCCCEEECCHHHEEEEECCCCCCCEEEECCCCHHHHHHHHEEE
VASGAQIIDINMGCPAKKVNRKLAGSALLQHPDLVKQILSAVVNAVDVPVTLKIRTGWSP
ECCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCC
EHRNCIEIAQLAENCGIQALTIHGRTRSCLFNGEAEYDSIRAVKQTVSIPVIANGDITDP
CCCHHHHHHHHHHHCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCC
HKARAVLDYTGADALMIGRAAQGRPWIFREIQHYLDTGELLPPMPLGEVQRLLDGHIREL
HHHCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
HDFYGPGKGFRIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENLA
HHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9811652 [H]