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The map label for this gene is mlaD [H]

Identifier: 22124064

GI number: 22124064

Start: 154816

End: 155388

Strand: Reverse

Name: mlaD [H]

Synonym: y0144

Alternate gene names: 22124064

Gene position: 155388-154816 (Counterclockwise)

Preceding gene: 22124065

Following gene: 22124063

Centisome position: 3.38

GC content: 48.17

Gene sequence:

>573_bases
TTGGGAACTGAGTCGATGCAAACGAAGAAAAGTGAAGTCTGGGTAGGAATGTTTATACTGATTGCTATTCTGGCAGTCAT
ATTTCTCTGCCTAAAAGTTGCAGATATCAAGTCAGTGGGCAATCAACCCACTTACCGTATTTACGCGAATTTTGACAATA
TTGGCGGCTTAAAAAACCACTCTCCTGTCAAAATTGGTGGCGTAGTTGTGGGGCGGGTGGCGGAAATCACCTTGGATACC
AAGAACTACACGCCACGCGTCGCGATAGATATTCAGCAACGCTATAACCACATCCCAGATACCAGCTCATTGGCTGTACG
CACATCGGGTTTACTGGGCGAACAATTCCTGGCACTGAATGTGGGTTTTGAAGATCCGGAAATGGGCACCAGTATTTTGA
AAGATGGCGGCACCATTCAAGACACCAAGTCTGCATTAGTTCTGGAAGATCTGATCGGCCAGTTCCTGTACAAGAGCAGC
GGTGATAGCCCGGCAGTAAGTGAAACGGCTCCGGCAACTGAAACTGCAGCTCCACAGGCTGCGGTACCTTCTCTTCCCGC
TCAACATCCTTAA

Upstream 100 bases:

>100_bases
GTCCCAACGTCTGAAGGGATTAGCCGGGCAACGACACGTACTGTAGTGCATTCGTCACTGGCGGTATTGGGATTGGATTT
TGTGCTGACAGCACTGATGT

Downstream 100 bases:

>100_bases
GAGGGCATCTGAATGTTTAAACGTCTATTCATGGTCGCATTGTTGGCCATAGCACCACTGGTTCATGCTGTCGATCAAAG
CAACCCTTATCGTCTGATGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MGTESMQTKKSEVWVGMFILIAILAVIFLCLKVADIKSVGNQPTYRIYANFDNIGGLKNHSPVKIGGVVVGRVAEITLDT
KNYTPRVAIDIQQRYNHIPDTSSLAVRTSGLLGEQFLALNVGFEDPEMGTSILKDGGTIQDTKSALVLEDLIGQFLYKSS
GDSPAVSETAPATETAAPQAAVPSLPAQHP

Sequences:

>Translated_190_residues
MGTESMQTKKSEVWVGMFILIAILAVIFLCLKVADIKSVGNQPTYRIYANFDNIGGLKNHSPVKIGGVVVGRVAEITLDT
KNYTPRVAIDIQQRYNHIPDTSSLAVRTSGLLGEQFLALNVGFEDPEMGTSILKDGGTIQDTKSALVLEDLIGQFLYKSS
GDSPAVSETAPATETAAPQAAVPSLPAQHP
>Mature_189_residues
GTESMQTKKSEVWVGMFILIAILAVIFLCLKVADIKSVGNQPTYRIYANFDNIGGLKNHSPVKIGGVVVGRVAEITLDTK
NYTPRVAIDIQQRYNHIPDTSSLAVRTSGLLGEQFLALNVGFEDPEMGTSILKDGGTIQDTKSALVLEDLIGQFLYKSSG
DSPAVSETAPATETAAPQAAVPSLPAQHP

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG1463

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, periplasmic component

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaD family [H]

Homologues:

Organism=Escherichia coli, GI1789584, Length=173, Percent_Identity=71.6763005780347, Blast_Score=248, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003399 [H]

Pfam domain/function: PF02470 MCE [H]

EC number: NA

Molecular weight: Translated: 20301; Mature: 20170

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTESMQTKKSEVWVGMFILIAILAVIFLCLKVADIKSVGNQPTYRIYANFDNIGGLKNH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC
SPVKIGGVVVGRVAEITLDTKNYTPRVAIDIQQRYNHIPDTSSLAVRTSGLLGEQFLALN
CCEEECCEEEEEEEEEEEECCCCCCEEEEEHHHHCCCCCCCCCEEEECCCCCCCEEEEEE
VGFEDPEMGTSILKDGGTIQDTKSALVLEDLIGQFLYKSSGDSPAVSETAPATETAAPQA
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
AVPSLPAQHP
CCCCCCCCCC
>Mature Secondary Structure 
GTESMQTKKSEVWVGMFILIAILAVIFLCLKVADIKSVGNQPTYRIYANFDNIGGLKNH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC
SPVKIGGVVVGRVAEITLDTKNYTPRVAIDIQQRYNHIPDTSSLAVRTSGLLGEQFLALN
CCEEECCEEEEEEEEEEEECCCCCCEEEEEHHHHCCCCCCCCCEEEECCCCCCCEEEEEE
VGFEDPEMGTSILKDGGTIQDTKSALVLEDLIGQFLYKSSGDSPAVSETAPATETAAPQA
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
AVPSLPAQHP
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]