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The map label for this gene is gltD [H]

Identifier: 22124049

GI number: 22124049

Start: 141374

End: 142792

Strand: Direct

Name: gltD [H]

Synonym: y0129

Alternate gene names: 22124049

Gene position: 141374-142792 (Clockwise)

Preceding gene: 161484918

Following gene: 22124050

Centisome position: 3.07

GC content: 51.37

Gene sequence:

>1419_bases
ATGAGTCAGAACGTTTATCAGTTTATCGACTTACAGCGCGTCGATCCGCCTAAAAAACCGCTGAAGATCCGTAAAATTGA
ATTTGTTGAAATTTACGAGCCATTCTCAGAAACACAAGCCAAAGCACAGGCCGATCGCTGCCTGTCCTGCGGCAATCCGT
ACTGTGAATGGAAATGCCCGGTTCATAACTACATCCCTAACTGGCTGAAACTGGCCAACGAGGGGCGGATTATGGAAGCG
GCTGATCTGGCACACCAAACGAACAGCCTACCGGAGGTCTGTGGCCGTGTTTGTCCACAAGATCGCTTGTGTGAAGGATC
TTGTACTCTGAACGACGAATTTGGTGCCGTGACCATCGGCAACATCGAGCGTTATATCAGTGACAAAGCCATTGCGATGG
GCTGGAAACCGGATATGTCACATGTTCACCCTACCGGTAAACGTGTGGCCGTGATCGGGGCCGGCCCTGCCGGGCTGGCC
TGTGCTGATGTATTAGCACGTAACGGTGTTCAGGCTGTGGTCTTTGACCGCCATCCAGAAATTGGGGGGTTACTGACCTT
CGGTATCCCAGCGTTTAAGCTGGAAAAAGAAGTGATGATTAAACGCCGTAAGATCTTCTCCGAGATGGGAATTGAATTCC
AACTGAATACTGAAGTAGGCAAAGACATCACCATGGAAGCACTGCTGAAAGATTACGACCCCGTGTTCCTGGGGGTTGGG
ACCTACCAATCTATGCGTGGTGGTTTGGAAAACGAAGAGGCTAACGGTGTATATGATGCCTTGCCTTTCCTGATTGCTAA
TACTAAACAGCTAATGGGCTATGAAGCGACGGCTCACGAACCGTATATCAACATGCAAGGTAAACGCGTCGTGGTGCTTG
GTGGCGGTGATACCGCAATGGACTGTGTGCGCTCCTCAATTCGTCAGGGGGCAACCGATGTGGTTTGCGCCTATCGCCGC
GATGAAGTGAATATGCCGGGTTCCAAGCGGGAAGTGAAAAACGCCCGTGAAGAAGGGGTAGAATTTAAATTCAACCTGCA
ACCATTAAGCATTGAAGTGAACAGCAATGGCAAAGTCTGTGGTGTCAGAATGGTCCGCACCCAGTTGGGCGCGCCAGATG
CACAGGGCCGGCGCATGGCTGAACAGATCCCTGGCTCAGAACATGTATTGCCAGCAGATGCCGTAGTGATGGCATTTGGT
TTCCGCCCACACAGTATGGAATGGCTGGCCGCTCACGATGTGAAGCTGGATAAGCAAGGCCGGGTGATTGCACCGGAAAG
CACTGATAATGCCTTCCAGACCAGTAACCCGAAAATCTTTGCGGGCGGCGATATTGTCCGTGGTTCCGATTTAGTGGTTA
CCGCCATTGCCGAAGGCCGTAAAGCCGCAGACGGCATCATGAATTATCTGGAAGTCTAA

Upstream 100 bases:

>100_bases
TGCCTTGGTTAAACCGAAATCCAGCGATGTGAAAGCACTGTTGGGCCACCGTAGCCGTTCCGCAGCTGAGCTGCGGGTTC
AAGCGCAGTAAGGAGTTGAT

Downstream 100 bases:

>100_bases
TGCGGTAGATCCCCAAATCATTCAATAAACGCTAGGGCGGGTTCAATTCACAGAACCCGCCCTTTCTATTTATTCTATTT
ATTTATGCACGTCTATTGTT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSETQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIMEA
ADLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYISDKAIAMGWKPDMSHVHPTGKRVAVIGAGPAGLA
CADVLARNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMIKRRKIFSEMGIEFQLNTEVGKDITMEALLKDYDPVFLGVG
TYQSMRGGLENEEANGVYDALPFLIANTKQLMGYEATAHEPYINMQGKRVVVLGGGDTAMDCVRSSIRQGATDVVCAYRR
DEVNMPGSKREVKNAREEGVEFKFNLQPLSIEVNSNGKVCGVRMVRTQLGAPDAQGRRMAEQIPGSEHVLPADAVVMAFG
FRPHSMEWLAAHDVKLDKQGRVIAPESTDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV

Sequences:

>Translated_472_residues
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSETQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIMEA
ADLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYISDKAIAMGWKPDMSHVHPTGKRVAVIGAGPAGLA
CADVLARNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMIKRRKIFSEMGIEFQLNTEVGKDITMEALLKDYDPVFLGVG
TYQSMRGGLENEEANGVYDALPFLIANTKQLMGYEATAHEPYINMQGKRVVVLGGGDTAMDCVRSSIRQGATDVVCAYRR
DEVNMPGSKREVKNAREEGVEFKFNLQPLSIEVNSNGKVCGVRMVRTQLGAPDAQGRRMAEQIPGSEHVLPADAVVMAFG
FRPHSMEWLAAHDVKLDKQGRVIAPESTDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV
>Mature_471_residues
SQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSETQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIMEAA
DLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYISDKAIAMGWKPDMSHVHPTGKRVAVIGAGPAGLAC
ADVLARNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMIKRRKIFSEMGIEFQLNTEVGKDITMEALLKDYDPVFLGVGT
YQSMRGGLENEEANGVYDALPFLIANTKQLMGYEATAHEPYINMQGKRVVVLGGGDTAMDCVRSSIRQGATDVVCAYRRD
EVNMPGSKREVKNAREEGVEFKFNLQPLSIEVNSNGKVCGVRMVRTQLGAPDAQGRRMAEQIPGSEHVLPADAVVMAFGF
RPHSMEWLAAHDVKLDKQGRVIAPESTDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=483, Percent_Identity=29.399585921325, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1789606, Length=472, Percent_Identity=84.3220338983051, Blast_Score=851, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=461, Percent_Identity=65.0759219088937, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI87082180, Length=450, Percent_Identity=57.3333333333333, Blast_Score=546, Evalue=1e-157,
Organism=Escherichia coli, GI1788468, Length=442, Percent_Identity=28.9592760180996, Blast_Score=175, Evalue=7e-45,
Organism=Escherichia coli, GI1789243, Length=422, Percent_Identity=31.9905213270142, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17570289, Length=489, Percent_Identity=30.879345603272, Blast_Score=219, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI71984108, Length=468, Percent_Identity=31.1965811965812, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17543860, Length=161, Percent_Identity=27.9503105590062, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=467, Percent_Identity=34.2612419700214, Blast_Score=239, Evalue=7e-64,
Organism=Drosophila melanogaster, GI28574881, Length=486, Percent_Identity=37.4485596707819, Blast_Score=285, Evalue=6e-77,
Organism=Drosophila melanogaster, GI24665539, Length=486, Percent_Identity=37.4485596707819, Blast_Score=285, Evalue=6e-77,
Organism=Drosophila melanogaster, GI24665547, Length=486, Percent_Identity=37.4485596707819, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24665543, Length=486, Percent_Identity=37.4485596707819, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24640763, Length=466, Percent_Identity=28.9699570815451, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI18858217, Length=466, Percent_Identity=28.9699570815451, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51969; Mature: 51838

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSETQAKAQADRCLSCGNPYCEWKCP
CCCCHHHHHHHHCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIMEAADLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIG
HHHHCCHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC
NIERYISDKAIAMGWKPDMSHVHPTGKRVAVIGAGPAGLACADVLARNGVQAVVFDRHPE
CHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMIKRRKIFSEMGIEFQLNTEVGKDITMEALLKDYDPVFLGVG
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCCEEEECC
TYQSMRGGLENEEANGVYDALPFLIANTKQLMGYEATAHEPYINMQGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCEEECCCCEEEEECCCCHHH
DCVRSSIRQGATDVVCAYRRDEVNMPGSKREVKNAREEGVEFKFNLQPLSIEVNSNGKVC
HHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEEEECCCCCEE
GVRMVRTQLGAPDAQGRRMAEQIPGSEHVLPADAVVMAFGFRPHSMEWLAAHDVKLDKQG
EEEEEEHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEECEEECCCC
RVIAPESTDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV
CEECCCCCCCCEECCCCEEEECCCEEECCCEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSETQAKAQADRCLSCGNPYCEWKCP
CCCHHHHHHHHCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIMEAADLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIG
HHHHCCHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC
NIERYISDKAIAMGWKPDMSHVHPTGKRVAVIGAGPAGLACADVLARNGVQAVVFDRHPE
CHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMIKRRKIFSEMGIEFQLNTEVGKDITMEALLKDYDPVFLGVG
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCCEEEECC
TYQSMRGGLENEEANGVYDALPFLIANTKQLMGYEATAHEPYINMQGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCEEECCCCEEEEECCCCHHH
DCVRSSIRQGATDVVCAYRRDEVNMPGSKREVKNAREEGVEFKFNLQPLSIEVNSNGKVC
HHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEEEECCCCCEE
GVRMVRTQLGAPDAQGRRMAEQIPGSEHVLPADAVVMAFGFRPHSMEWLAAHDVKLDKQG
EEEEEEHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEECEEECCCC
RVIAPESTDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV
CEECCCCCCCCEECCCCEEEECCCEEECCCEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]