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The map label for this gene is gltS [H]

Identifier: 22124026

GI number: 22124026

Start: 114221

End: 115435

Strand: Reverse

Name: gltS [H]

Synonym: y0106

Alternate gene names: 22124026

Gene position: 115435-114221 (Counterclockwise)

Preceding gene: 22124029

Following gene: 22124020

Centisome position: 2.51

GC content: 50.04

Gene sequence:

>1215_bases
ATGTTTCATCTCGATACCTATGGCACGCTAGTTGCGGCCTGTCTGGTTTTACTGTTAGGCCGAAAACTTGTACAAACCGT
CCCTTTCCTAAAGAAATACACTATCCCTGAACCCGTAGCCGGTGGTTTGCTGGTGGCTTTTATGATGCTTCTGGTACAGA
AGACGCTGGGCTGGGAAGTCAGTTTTGATATGTCTCTGAAAGACCCTCTGATGCTAGCCTTCTTTGCCACGATAGGTTTG
AACGCCAACTTGGCCAGTTTACGGGCTGGAGGTAAGGCTCTGAGCATATTTGTCTTTATCGTTGTTGGCTTACTGCTGGT
GCAAAACACGATTGGGATTGTGCTGGCTAAATTGATGGGGCTGGATCCCTTAATGGGTCTCTTGGCTGGGTCCATCACAT
TATCGGGTGGGCACGGCACTGGTGCTGCGTGGAGTAAATTATTCTCTGAGCGTTACGGATTTGAAAACGCAACAGAAGTT
GCCATGGCTTGTGCAACCTTTGGTTTGGTATTGGGTGGGTTGATTGGTGGCCCGGTCGCTCGTTATCTGGTGAAGCACTC
TTCCACGCCAGAGGGTACCCCAGATGATAGCGCCGTCCCCTCTGCTTTTGAGAAGCCCTCTGCTGGCCGTATGATCACAT
CACTGGTGTTGATAGAAACCATCGCCATGATCTCTATTTGCCTGATGGCCGGTAATCTGATTGCAGGTTGGTTGCAAGGG
ACGATGTTCGAGTTACCGATCTTTGTTTGCGTACTATTTGTCGGAGTAATCCTTAGCAATACGCTGTCAGCCAGCGGCTT
CTATAGAGTCTTTGACCGTGCGGTATCCGTGTTGGGGAATGTCAGTTTATCATTGTTCCTGGCCATGGCGCTGATGAGCC
TGAAGCTGTGGGAGCTGGCTTCATTAGCATTACCGATGTTGGTGATTTTGTCGGTACAGGCGTTGGCCATGGCACTGTAC
GCGATATTTGTCACCTACCGTATCATGGGCAAAAACTATGATGCAGCGGTATTAGCGGCAGGACACTGTGGCTTCGGTTT
GGGGGCTACGCCAACCGCGATTGCCAATATGCAAGCCATCACCGACCGGTTTGGCCCATCTCATTTGGCGTTCTTGGTGG
TTCCGATGGTTGGCGCGTTCTTTATTGATATCGTCAATGCGATTGTGATCAAGCTTTATCTGTTGTTGCCAATATTTCCA
GCCGTAACGGGGTGA

Upstream 100 bases:

>100_bases
CATTTTTTTTTCTAATCCTCTCTTCTATTACTTTGGCGTTTTTTAAGGAACTGTCTAACGTCATATTTTTATTAGATATT
TTATTGCATGGAGTACCAAG

Downstream 100 bases:

>100_bases
TAGCTCCGTCATGCTGCGGGTAGCATATACGTTAGTTATCCTCGCTCACACCAGTTACTTTCTTATGTGAGTGGCTGGGA
ATTAAGAAGCCTCATGAATG

Product: sodium/glutamate symport carrier protein

Products: Na (I) [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate permease [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MFHLDTYGTLVAACLVLLLGRKLVQTVPFLKKYTIPEPVAGGLLVAFMMLLVQKTLGWEVSFDMSLKDPLMLAFFATIGL
NANLASLRAGGKALSIFVFIVVGLLLVQNTIGIVLAKLMGLDPLMGLLAGSITLSGGHGTGAAWSKLFSERYGFENATEV
AMACATFGLVLGGLIGGPVARYLVKHSSTPEGTPDDSAVPSAFEKPSAGRMITSLVLIETIAMISICLMAGNLIAGWLQG
TMFELPIFVCVLFVGVILSNTLSASGFYRVFDRAVSVLGNVSLSLFLAMALMSLKLWELASLALPMLVILSVQALAMALY
AIFVTYRIMGKNYDAAVLAAGHCGFGLGATPTAIANMQAITDRFGPSHLAFLVVPMVGAFFIDIVNAIVIKLYLLLPIFP
AVTG

Sequences:

>Translated_404_residues
MFHLDTYGTLVAACLVLLLGRKLVQTVPFLKKYTIPEPVAGGLLVAFMMLLVQKTLGWEVSFDMSLKDPLMLAFFATIGL
NANLASLRAGGKALSIFVFIVVGLLLVQNTIGIVLAKLMGLDPLMGLLAGSITLSGGHGTGAAWSKLFSERYGFENATEV
AMACATFGLVLGGLIGGPVARYLVKHSSTPEGTPDDSAVPSAFEKPSAGRMITSLVLIETIAMISICLMAGNLIAGWLQG
TMFELPIFVCVLFVGVILSNTLSASGFYRVFDRAVSVLGNVSLSLFLAMALMSLKLWELASLALPMLVILSVQALAMALY
AIFVTYRIMGKNYDAAVLAAGHCGFGLGATPTAIANMQAITDRFGPSHLAFLVVPMVGAFFIDIVNAIVIKLYLLLPIFP
AVTG
>Mature_404_residues
MFHLDTYGTLVAACLVLLLGRKLVQTVPFLKKYTIPEPVAGGLLVAFMMLLVQKTLGWEVSFDMSLKDPLMLAFFATIGL
NANLASLRAGGKALSIFVFIVVGLLLVQNTIGIVLAKLMGLDPLMGLLAGSITLSGGHGTGAAWSKLFSERYGFENATEV
AMACATFGLVLGGLIGGPVARYLVKHSSTPEGTPDDSAVPSAFEKPSAGRMITSLVLIETIAMISICLMAGNLIAGWLQG
TMFELPIFVCVLFVGVILSNTLSASGFYRVFDRAVSVLGNVSLSLFLAMALMSLKLWELASLALPMLVILSVQALAMALY
AIFVTYRIMGKNYDAAVLAAGHCGFGLGATPTAIANMQAITDRFGPSHLAFLVVPMVGAFFIDIVNAIVIKLYLLLPIFP
AVTG

Specific function: Catalyzes the sodium-dependent uptake of extracellular glutamate [H]

COG id: COG0786

COG function: function code E; Na+/glutamate symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gltS symporter family [H]

Homologues:

Organism=Escherichia coli, GI1790085, Length=399, Percent_Identity=82.4561403508772, Blast_Score=598, Evalue=1e-172,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004445 [H]

Pfam domain/function: PF03616 Glt_symporter [H]

EC number: NA

Molecular weight: Translated: 42726; Mature: 42726

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHLDTYGTLVAACLVLLLGRKLVQTVPFLKKYTIPEPVAGGLLVAFMMLLVQKTLGWEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCE
SFDMSLKDPLMLAFFATIGLNANLASLRAGGKALSIFVFIVVGLLLVQNTIGIVLAKLMG
EECCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDPLMGLLAGSITLSGGHGTGAAWSKLFSERYGFENATEVAMACATFGLVLGGLIGGPVA
CHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RYLVKHSSTPEGTPDDSAVPSAFEKPSAGRMITSLVLIETIAMISICLMAGNLIAGWLQG
HHHHHCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TMFELPIFVCVLFVGVILSNTLSASGFYRVFDRAVSVLGNVSLSLFLAMALMSLKLWELA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLALPMLVILSVQALAMALYAIFVTYRIMGKNYDAAVLAAGHCGFGLGATPTAIANMQAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCCCCCCHHHHHHHHHH
TDRFGPSHLAFLVVPMVGAFFIDIVNAIVIKLYLLLPIFPAVTG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFHLDTYGTLVAACLVLLLGRKLVQTVPFLKKYTIPEPVAGGLLVAFMMLLVQKTLGWEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCE
SFDMSLKDPLMLAFFATIGLNANLASLRAGGKALSIFVFIVVGLLLVQNTIGIVLAKLMG
EECCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDPLMGLLAGSITLSGGHGTGAAWSKLFSERYGFENATEVAMACATFGLVLGGLIGGPVA
CHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RYLVKHSSTPEGTPDDSAVPSAFEKPSAGRMITSLVLIETIAMISICLMAGNLIAGWLQG
HHHHHCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TMFELPIFVCVLFVGVILSNTLSASGFYRVFDRAVSVLGNVSLSLFLAMALMSLKLWELA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLALPMLVILSVQALAMALYAIFVTYRIMGKNYDAAVLAAGHCGFGLGATPTAIANMQAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCCCCCCHHHHHHHHHH
TDRFGPSHLAFLVVPMVGAFFIDIVNAIVIKLYLLLPIFPAVTG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-glutamate [Periplasm] = Na (I) [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254324; 2017136; 7686882; 9278503; 1544582 [H]