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The map label for this gene is pfkA [H]

Identifier: 22123980

GI number: 22123980

Start: 72108

End: 73091

Strand: Reverse

Name: pfkA [H]

Synonym: y0059

Alternate gene names: 22123980

Gene position: 73091-72108 (Counterclockwise)

Preceding gene: 22123981

Following gene: 22123979

Centisome position: 1.59

GC content: 47.15

Gene sequence:

>984_bases
ATGGTTAAGAAAATTGGTGTCCTAACAAGTGGTGGTGATGCACCCGGTATGAATGCTGCTATTCGCGGGGTTGTTCGTGC
CGCTTTGTCAGCAGGGTTAGACGTTTTCGGTATTGAAGATGGCTATCTTGGCTTATATGAAAATCGTATGAAGAAGCTGG
ATCGCTATAGCGTGTCAGATATGATTAACCGTGGCGGTACTTTCCTCGGATCTGCTCGTTTCCCAGAATTCCGCGATCCG
GAAGTGCGTAAAGTTGCCCTCAAGAATATGCATGAGCGTGGCATTGATGGCTTGGTTGTTATCGGTGGTGATGGTTCTTA
TGCTGGTGCGGACCTGCTGACAAAAGAAGGGGGAATTCACTGTGTTGGTTTACCGGGCACTATCGATAACGATGTGGCGG
GTACTGACTACACCATCGGTTTTTTCACCGCACTGGAAACGGTAGTGGAAGCCATTGACCGCTTACGCGATACCTCTTCT
TCCCACCAACGTATCTCTATCGTGGAAGTGATGGGCCGCTATTGCGGTGATTTAACGTTGGCAGCCGCGATTGCCGGTGG
TTGTGAGTTTATTGCGATCCCTGAAGTTGAATTTAAACGTGATGATCTGGTTGCTGAAATCAAAGCGGGCATCGCGAAAG
GTAAAAAGCACGCGATTGTTGCTATTACTGAAAAGCTGGATGATATCGATTCATTGGCTAAGTACATTGAAAAAGAGACG
GGCCGTGAAACTCGTGGCACCGTGTTAGGCCATATCCAACGTGGTGGTGCTCCGGTGGCTTATGACCGTATTCTTGCTTC
CCGTATGGGGGCTTATGCTGTCGATTTGCTGTTACAGGACCACGATTACAAAAAAGGTGGTTTCTGCGTCGGTGTACAGA
ATGAGAAGATGGTGCATGAGTTAATTTCTGTGTGTATCGCGCCAGAGAATAAGAAAAGTAAATTTAAAGAAGATTGGTAC
GATACAGCGAAGAAACTGTTCTAA

Upstream 100 bases:

>100_bases
TAATCCCATAAAGCTGATTTTCTGTACGGTCTCTTCCGTGCAAAGATAATCGGCAGATAAAGAATTCTACAAAATCGCAT
TTACAAGTTCAGAGGTAGTC

Downstream 100 bases:

>100_bases
AACGGCAACTCGCTCATTCATCAAGCCCTCGAAATTGAACTGATCCCAAAAAGTTGGGCAGTTTACATTACGAGGGCTTT
TTACTTTCGGTCACCCCTAT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MVKKIGVLTSGGDAPGMNAAIRGVVRAALSAGLDVFGIEDGYLGLYENRMKKLDRYSVSDMINRGGTFLGSARFPEFRDP
EVRKVALKNMHERGIDGLVVIGGDGSYAGADLLTKEGGIHCVGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSS
SHQRISIVEVMGRYCGDLTLAAAIAGGCEFIAIPEVEFKRDDLVAEIKAGIAKGKKHAIVAITEKLDDIDSLAKYIEKET
GRETRGTVLGHIQRGGAPVAYDRILASRMGAYAVDLLLQDHDYKKGGFCVGVQNEKMVHELISVCIAPENKKSKFKEDWY
DTAKKLF

Sequences:

>Translated_327_residues
MVKKIGVLTSGGDAPGMNAAIRGVVRAALSAGLDVFGIEDGYLGLYENRMKKLDRYSVSDMINRGGTFLGSARFPEFRDP
EVRKVALKNMHERGIDGLVVIGGDGSYAGADLLTKEGGIHCVGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSS
SHQRISIVEVMGRYCGDLTLAAAIAGGCEFIAIPEVEFKRDDLVAEIKAGIAKGKKHAIVAITEKLDDIDSLAKYIEKET
GRETRGTVLGHIQRGGAPVAYDRILASRMGAYAVDLLLQDHDYKKGGFCVGVQNEKMVHELISVCIAPENKKSKFKEDWY
DTAKKLF
>Mature_327_residues
MVKKIGVLTSGGDAPGMNAAIRGVVRAALSAGLDVFGIEDGYLGLYENRMKKLDRYSVSDMINRGGTFLGSARFPEFRDP
EVRKVALKNMHERGIDGLVVIGGDGSYAGADLLTKEGGIHCVGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSS
SHQRISIVEVMGRYCGDLTLAAAIAGGCEFIAIPEVEFKRDDLVAEIKAGIAKGKKHAIVAITEKLDDIDSLAKYIEKET
GRETRGTVLGHIQRGGAPVAYDRILASRMGAYAVDLLLQDHDYKKGGFCVGVQNEKMVHELISVCIAPENKKSKFKEDWY
DTAKKLF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=311, Percent_Identity=40.8360128617363, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI266453768, Length=311, Percent_Identity=41.4790996784566, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI266453748, Length=311, Percent_Identity=41.4790996784566, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI4505749, Length=311, Percent_Identity=41.4790996784566, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI266453619, Length=311, Percent_Identity=41.4790996784566, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI48762920, Length=311, Percent_Identity=41.8006430868167, Blast_Score=208, Evalue=6e-54,
Organism=Escherichia coli, GI1790350, Length=327, Percent_Identity=77.0642201834862, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=40.1929260450161, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17558788, Length=319, Percent_Identity=36.9905956112853, Blast_Score=187, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI72003601, Length=291, Percent_Identity=31.2714776632302, Blast_Score=134, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6321679, Length=311, Percent_Identity=42.443729903537, Blast_Score=210, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323861, Length=313, Percent_Identity=38.0191693290735, Blast_Score=185, Evalue=8e-48,
Organism=Drosophila melanogaster, GI28573326, Length=312, Percent_Identity=42.3076923076923, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI17647809, Length=311, Percent_Identity=42.1221864951768, Blast_Score=217, Evalue=9e-57,
Organism=Drosophila melanogaster, GI24652337, Length=311, Percent_Identity=42.1221864951768, Blast_Score=217, Evalue=1e-56,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35395; Mature: 35395

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKIGVLTSGGDAPGMNAAIRGVVRAALSAGLDVFGIEDGYLGLYENRMKKLDRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPEFRDPEVRKVALKNMHERGIDGLVVIGGDGSYAGADLLTKEGGIH
HHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHEECCCCEE
CVGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSSSHQRISIVEVMGRYCGDLTL
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHH
AAAIAGGCEFIAIPEVEFKRDDLVAEIKAGIAKGKKHAIVAITEKLDDIDSLAKYIEKET
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHC
GRETRGTVLGHIQRGGAPVAYDRILASRMGAYAVDLLLQDHDYKKGGFCVGVQNEKMVHE
CCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHH
LISVCIAPENKKSKFKEDWYDTAKKLF
HHHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKKIGVLTSGGDAPGMNAAIRGVVRAALSAGLDVFGIEDGYLGLYENRMKKLDRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPEFRDPEVRKVALKNMHERGIDGLVVIGGDGSYAGADLLTKEGGIH
HHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHEECCCCEE
CVGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSSSHQRISIVEVMGRYCGDLTL
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHH
AAAIAGGCEFIAIPEVEFKRDDLVAEIKAGIAKGKKHAIVAITEKLDDIDSLAKYIEKET
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHC
GRETRGTVLGHIQRGGAPVAYDRILASRMGAYAVDLLLQDHDYKKGGFCVGVQNEKMVHE
CCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHH
LISVCIAPENKKSKFKEDWYDTAKKLF
HHHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA