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The map label for this gene is adhB [H]

Identifier: 221232592

GI number: 221232592

Start: 1817528

End: 1818565

Strand: Reverse

Name: adhB [H]

Synonym: SPN23F_18700

Alternate gene names: 221232592

Gene position: 1818565-1817528 (Counterclockwise)

Preceding gene: 221232593

Following gene: 221232590

Centisome position: 81.87

GC content: 44.8

Gene sequence:

>1038_bases
ATGAAATCAGCAGTATATACAAAGGCAGGTCAGGTTGGACTTGCTAGCATTGAACGTCCGCAAATAATAGAAGCGGATGA
TGTGATTATTCGTGTGGTTCGTGCGTGCGTTTGTGGTTCAGATTTATGGAGGTACCGTAATCCAGAAACGAAAGCTGGAC
ATAAAAATAGTGGACACGAAGCGATTGGGATTGTTGAAGAAGCTGGGGAAGCCATTACGACGGTGAAACCAGGTGATTTT
GTGATTGTCCCTTTTACACATGGATGTGGTGAGTGTGATGCCTGTCTTGCTGGATTTGACGGTTCTTGCGACAATCATAT
TGGTAATAATTTGGGGGGTGATTTTCAGGCAGAATATATTCGCTTCCACTATGCAAACTGGGCGCTGGTTAAAATCCCTG
GTCAACCTTCTGATTATACAGAAGGGATGCTCAAGTCCCTTTTGACTCTTGCAGATGTCATGCCGACAGGCTATCATGCA
GCGCGTGTTGCAAATGTTCAAAAAGGGGACAAGGTTGTTGTTATCGGTGATGGGGCTGTTGGTCAATGTGCTGTCATCGC
GGCTAAGATGCGTGGAGCATCACAAATTATCCTTATGAGCCGTCATGAAGACCGTCAAAAGATGGCTATGGAGTCAGGTG
CGACAGCTGTTGTTGCAGAACGTGGTCAAGAAGGAATTACCAAGGTGCGTGAAATCCTCGGCGGAGGAGCAGACGCAGCA
CTTGAATGTGTTGGTACGGAGGCTGCTATAGAACAGGCGCTAGGTGTTCTTCATAATGGAGGGCGTATGGGTTTTGTAGG
AGTCCCACACTATAATAATCGTGCTCTTGGTTCGACATTTATGCAAAATATCTCTGTAGCAGGTGGGGCAGCTTCTGCTA
CAACATACGATAAGCAATTTTTACTAAAAGCCGTCCTTGATGGTGATATCAATCCAGGTCGCGTCTTTACTTCAAGTTAT
AAACTGGAAGATATCGACCAAGCCTATAAAGATATGGATGAACGTAAGACAATTAAGTCTATGATTGTAATCGAATAA

Upstream 100 bases:

>100_bases
AGAGGAAAAGCAAAAATTAGAGGAGCGCTTGTCTCAGCTACAGACAGCTTTAAATCGTTTAAATCTCAAAATTAAACTTT
ATAAGGAAGGAAAATTTTAA

Downstream 100 bases:

>100_bases
AAAACGAATAGGAGTTTTAGAACTCTATTCGTTTTTTATGTTATCCTATTCTTGATTTAGGGTACTTTCTCTTAATGTCA
GTCTGGTTCCCAGCATGGTC

Product: zinc-binding dehydrogenase

Products: aldehyde; ketone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWRYRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDF
VIVPFTHGCGECDACLAGFDGSCDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVVAERGQEGITKVREILGGGADAA
LECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRALGSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSY
KLEDIDQAYKDMDERKTIKSMIVIE

Sequences:

>Translated_345_residues
MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWRYRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDF
VIVPFTHGCGECDACLAGFDGSCDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVVAERGQEGITKVREILGGGADAA
LECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRALGSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSY
KLEDIDQAYKDMDERKTIKSMIVIE
>Mature_345_residues
MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWRYRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDF
VIVPFTHGCGECDACLAGFDGSCDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVVAERGQEGITKVREILGGGADAA
LECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRALGSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSY
KLEDIDQAYKDMDERKTIKSMIVIE

Specific function: Unknown

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501933, Length=351, Percent_Identity=28.2051282051282, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI71565154, Length=373, Percent_Identity=25.7372654155496, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI34577061, Length=309, Percent_Identity=28.8025889967638, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI4501929, Length=353, Percent_Identity=27.1954674220963, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI156627571, Length=327, Percent_Identity=26.605504587156, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI156523966, Length=381, Percent_Identity=26.7716535433071, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4501939, Length=372, Percent_Identity=26.8817204301075, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786825, Length=387, Percent_Identity=26.6149870801034, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788073, Length=353, Percent_Identity=26.3456090651558, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082125, Length=339, Percent_Identity=26.2536873156342, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788075, Length=351, Percent_Identity=27.0655270655271, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790045, Length=341, Percent_Identity=24.9266862170088, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786552, Length=363, Percent_Identity=27.5482093663912, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788407, Length=291, Percent_Identity=26.4604810996564, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081918, Length=350, Percent_Identity=26.8571428571429, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI226510992, Length=327, Percent_Identity=22.0183486238532, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787863, Length=351, Percent_Identity=22.7920227920228, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790718, Length=329, Percent_Identity=22.1884498480243, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=328, Percent_Identity=25.609756097561, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=24.6648793565684, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71997431, Length=322, Percent_Identity=24.8447204968944, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17565904, Length=226, Percent_Identity=29.2035398230088, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17562878, Length=338, Percent_Identity=23.6686390532544, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320033, Length=346, Percent_Identity=26.878612716763, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319258, Length=387, Percent_Identity=24.031007751938, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6319955, Length=340, Percent_Identity=26.1764705882353, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322619, Length=340, Percent_Identity=26.1764705882353, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17737897, Length=343, Percent_Identity=26.8221574344023, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17137530, Length=247, Percent_Identity=28.7449392712551, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17737895, Length=360, Percent_Identity=25.5555555555556, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.1

Molecular weight: Translated: 36782; Mature: 36782

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00059 ADH_ZINC ; PS00222 IGF_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWRYRNPETKAGHKNSGHE
CCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCH
AIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGSCDNHIGNNLGGDFQAEYI
HEEEHHHCCCEEEEECCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
RFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHAARVANVQKGDKVVVIGDGAV
EEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCCC
GQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVVAERGQEGITKVREILGGGADAA
CHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCEEEEHHCCHHHHHHHHHHHCCCCCHH
LECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRALGSTFMQNISVAGGAASATTYDKQF
HHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHH
LLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDERKTIKSMIVIE
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWRYRNPETKAGHKNSGHE
CCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCH
AIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGSCDNHIGNNLGGDFQAEYI
HEEEHHHCCCEEEEECCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
RFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHAARVANVQKGDKVVVIGDGAV
EEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCCC
GQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVVAERGQEGITKVREILGGGADAA
CHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCEEEEHHCCHHHHHHHHHHHCCCCCHH
LECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRALGSTFMQNISVAGGAASATTYDKQF
HHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHH
LLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDERKTIKSMIVIE
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: alcohol; NAD+

Specific reaction: an alcohol + NAD+ = an aldehyde or ketone + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9141695; 9384377 [H]