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The map label for this gene is rsmE [H]

Identifier: 221232521

GI number: 221232521

Start: 1741813

End: 1742556

Strand: Reverse

Name: rsmE [H]

Synonym: SPN23F_17940

Alternate gene names: 221232521

Gene position: 1742556-1741813 (Counterclockwise)

Preceding gene: 221232522

Following gene: 221232520

Centisome position: 78.45

GC content: 44.35

Gene sequence:

>744_bases
ATGCAGCAGTATTTTGTAAAAGGCAGTGCTATCTCTCCTGTCACTATCGAGGACAAGGAAATCAGTAAGCATATGTTTCA
GGTTATGCGCTTGAAAGAAGAGGATGAGGTTACCTTAGTCTTTGATGATGGCATCAAGCGCTTGGCGCGCGTGCTGGATA
TGGAAAATCGTCAGTTTGAGTTGGTCCAAGAATTAAATGACAATGTGGAACTACCAGTCCAAGTGACCATCGCATCTGGA
TTTCCCAAGGGAGACAAGCTGGAGTTCATCGCTCAAAAAGTAACCGAACTGGGTGCTAGCCAAATCTGGGCCTTTCCTGC
AGACTGGTCAGTTGCCAAGTGGGATGGCAAGAAATTGGGTAAAAAAGTTGAAAAACTAGAAAAAATTGCCCTTGGAGCAG
CCGAGCAAAGCAAGCGTAATATTGTACCAAGTATTAAACTTTTCGAGAAAAAAGCAGATTTTCTAGCTCAGCTGGACCAG
TTTGACTCTATCATAGTAGCCTATGAAGAATCAGCTAAAGAAGGAGAAGCCGCTGCGCTTCTGCAAGCAGTCACTGGTCT
TGAAACAGGAGCCAAACTGCTCTTTATCTTTGGTCCAGAAGGTGGTCTCTCACCTGCAGAAATCGAAAGTTTTGAAGCTA
AAGGAGCAGTTTTGGCAGGCCTAGGTCCTCGTATTTTGCGAGCAGAAACAGCACCGCTTTACGCCTTATCAGCCCTTAGT
GTTTTAGTAGAATTAGAGAAATAA

Upstream 100 bases:

>100_bases
TCGGCTGGATTTTTCCTCGAAACCCACATGATTCAAGGGGAGTGGAATGCCTGTGTCTTTAAGAAGACCAAGGATATTTC
AGGGGTGATTGGAGGCTAGC

Downstream 100 bases:

>100_bases
GAGGAAGAAAATGGAACAAAAACACCGTTCAGAATTTCCAGAGAAGGAACTCTGGGACTTAACAGCCCTATACCAAGACC
GTGAGGATTTCTTGCGTGCA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MQQYFVKGSAISPVTIEDKEISKHMFQVMRLKEEDEVTLVFDDGIKRLARVLDMENRQFELVQELNDNVELPVQVTIASG
FPKGDKLEFIAQKVTELGASQIWAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLAGLGPRILRAETAPLYALSALS
VLVELEK

Sequences:

>Translated_247_residues
MQQYFVKGSAISPVTIEDKEISKHMFQVMRLKEEDEVTLVFDDGIKRLARVLDMENRQFELVQELNDNVELPVQVTIASG
FPKGDKLEFIAQKVTELGASQIWAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLAGLGPRILRAETAPLYALSALS
VLVELEK
>Mature_247_residues
MQQYFVKGSAISPVTIEDKEISKHMFQVMRLKEEDEVTLVFDDGIKRLARVLDMENRQFELVQELNDNVELPVQVTIASG
FPKGDKLEFIAQKVTELGASQIWAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLAGLGPRILRAETAPLYALSALS
VLVELEK

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=175, Percent_Identity=32, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27168; Mature: 27168

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYFVKGSAISPVTIEDKEISKHMFQVMRLKEEDEVTLVFDDGIKRLARVLDMENRQFE
CCCEEECCCCCCCEEECHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCHHH
LVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQIWAFPADWSVAKWDGKKLG
HHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHEEECCCCCCEEECCHHHHH
KKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQFDSIIVAYEESAKEGEAAAL
HHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHH
LQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLAGLGPRILRAETAPLYALSALS
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCHHEECCCCCHHHHHHHH
VLVELEK
HHHEECC
>Mature Secondary Structure
MQQYFVKGSAISPVTIEDKEISKHMFQVMRLKEEDEVTLVFDDGIKRLARVLDMENRQFE
CCCEEECCCCCCCEEECHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCHHH
LVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQIWAFPADWSVAKWDGKKLG
HHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHEEECCCCCCEEECCHHHHH
KKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQFDSIIVAYEESAKEGEAAAL
HHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHH
LQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLAGLGPRILRAETAPLYALSALS
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCHHEECCCCCHHHHHHHH
VLVELEK
HHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]