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The map label for this gene is rfaI [H]

Identifier: 221232503

GI number: 221232503

Start: 1711240

End: 1712460

Strand: Reverse

Name: rfaI [H]

Synonym: SPN23F_17750

Alternate gene names: 221232503

Gene position: 1712460-1711240 (Counterclockwise)

Preceding gene: 221232504

Following gene: 221232502

Centisome position: 77.09

GC content: 34.15

Gene sequence:

>1221_bases
ATGTTACAAGATAAGAAATCTGTAGTATTTGCAGGTGATTACGCTTATATTCGACAAATCGAAACGGCGATGAAGTCACT
CTGTAGACACAATAGTCATTTGAAAATTTATCTGCTAAATCAGGACATTCCTCAGGAATGGTTTAGTCAAATAAGAATAT
ATTTACAAGAGATGGGGGGCGACTTGATTGACTGCAAGTTAATTGGCTCACAGTTTCAAATGAATTGGTCTAATAAATTA
CCTCATATCAATCATATGACATTTGCACGCTATTTTATTCCAGATTTTGTAACAGAAGATAAAGTTCTCTATCTAGATAG
TGATTTGATTGTGACTGGTGATTTGACCGATTTGTTTGAATTAGACTTAGGTGAAAATTATTTGGCAGCAGCTCGTTCTT
GCTTTGGAGCAGGAGTCGGCTTCAATGCTGGTGTTCTCTTGATTAACAACAAAAAATGGGGATCTGAAACTATTCGACAA
AAATTGATTGACTTAACAGAAAAAGAACATGAGAATGTGGAAGAAGGAGACCAGTCAATTTTGAATATGTTGTTTAAAGA
TCAATATAGTTCCCTTGAAGATCAATATAATTTTCAAATAGGATATGATTATGGGGCGGCAGCCTTTAAACATCAATTCA
TTTTTGATATTCCGCTCGAACCACTGCCACTAATTTTACACTATATTTCTCAGGATAAGCCTTGGAATCAATTTTCTGTT
GGACGTCTAAGAGAAGTTTGGTGGGAATACTCTTTGATGGATTGGTCTGTTATTTTAAATGAATGGTTTTCAAAGAGTGT
GAAGTATCCTAGTAAATCACAAATATTTAAGTTGCAATGTGTTAATTTAACGAATTCTTGGTGTGTCGAGAAAATCGATT
ATTTGGCGGAGCAATTGCCAGAAGTTCATTTTCATATTGTTGCTTATACAAATATGGCAAATGAACTACTAGCTTTAACG
CGTTTTCCTAATGTTACCGTATATCCAAATTCCTTACCAATGTTATTGGAACAAATAGTAATAGCTTCAGATTTGTATTT
GGATTTGAATCATGATCGAAAATTAGAAGATGCATATGAGTTTGTGCTTAAGTACAAAAAACCAATGATAGCTTTCGACA
ATACTTGCTCTGAAAATCTTTCTGAGATTTCATATGAAGGTATCTATCCAAGCTCCATTCCGAAAAAAATGGTTGCAGCA
ATCAGATCTTACATGAGGTAG

Upstream 100 bases:

>100_bases
AGAACTTAAGGAGAATGTGGAAACAAGACATTTAGAAATGTTAGACTATTTATTGAATGGTAATAAATATAACTGAAAAT
TAAGATAGAGAGACGATAAA

Downstream 100 bases:

>100_bases
AGAACAGTATGAGAAAATCAATAGTATTAGCGGCAGATAATGTCTATCTTATTCCTTTAGAGACGACTATAAAGTCTGTA
TTGTATCACAATAGAGATGT

Product: glycosyltransferase

Products: NA

Alternate protein names: Lipopolysaccharide 3-alpha-galactosyltransferase [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MLQDKKSVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIPQEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKL
PHINHMTFARYFIPDFVTEDKVLYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIPLEPLPLILHYISQDKPWNQFSV
GRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIFKLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALT
RFPNVTVYPNSLPMLLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSSIPKKMVAA
IRSYMR

Sequences:

>Translated_406_residues
MLQDKKSVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIPQEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKL
PHINHMTFARYFIPDFVTEDKVLYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIPLEPLPLILHYISQDKPWNQFSV
GRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIFKLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALT
RFPNVTVYPNSLPMLLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSSIPKKMVAA
IRSYMR
>Mature_406_residues
MLQDKKSVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIPQEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKL
PHINHMTFARYFIPDFVTEDKVLYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIPLEPLPLILHYISQDKPWNQFSV
GRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIFKLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALT
RFPNVTVYPNSLPMLLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSSIPKKMVAA
IRSYMR

Specific function: Adds the galactose(I) group on the glucose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

Organism=Escherichia coli, GI1790057, Length=211, Percent_Identity=27.4881516587678, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.44 [H]

Molecular weight: Translated: 47356; Mature: 47356

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQDKKSVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIPQEWFSQIRIYLQEMGG
CCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
DLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKVLYLDSDLIVTGDLTDLFE
CEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCHHHHH
LDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQKLIDLTEKEHENVEEGDQSI
HCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
LNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIPLEPLPLILHYISQDKPWNQFSV
HHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCEEEECCCCHHHHHHHHHHCCCCCCCCHH
GRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIFKLQCVNLTNSWCVEKIDYLAEQLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCC
EVHFHIVAYTNMANELLALTRFPNVTVYPNSLPMLLEQIVIASDLYLDLNHDRKLEDAYE
CCEEEEEEEHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHH
FVLKYKKPMIAFDNTCSENLSEISYEGIYPSSIPKKMVAAIRSYMR
HHHHHCCCCEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLQDKKSVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIPQEWFSQIRIYLQEMGG
CCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
DLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKVLYLDSDLIVTGDLTDLFE
CEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCHHHHH
LDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQKLIDLTEKEHENVEEGDQSI
HCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
LNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIPLEPLPLILHYISQDKPWNQFSV
HHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCEEEECCCCHHHHHHHHHHCCCCCCCCHH
GRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIFKLQCVNLTNSWCVEKIDYLAEQLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCC
EVHFHIVAYTNMANELLALTRFPNVTVYPNSLPMLLEQIVIASDLYLDLNHDRKLEDAYE
CCEEEEEEEHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHH
FVLKYKKPMIAFDNTCSENLSEISYEGIYPSSIPKKMVAAIRSYMR
HHHHHCCCCEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]