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The map label for this gene is fliY [H]

Identifier: 221232213

GI number: 221232213

Start: 1418667

End: 1419503

Strand: Reverse

Name: fliY [H]

Synonym: SPN23F_14650

Alternate gene names: 221232213

Gene position: 1419503-1418667 (Counterclockwise)

Preceding gene: 221232214

Following gene: 221232210

Centisome position: 63.9

GC content: 37.28

Gene sequence:

>837_bases
ATGAAAAAATGGATGCTTGTATTAGTCAGTCTGATGACTGCTTTGTTCTTAGTAGCTTGTGGGAAAAATTCTAGCGAAAC
TAGTGGAGATAATTGGTCAAAGTACCAGTCTAACAAGTCTATTACTATTGGATTTGATAGTACTTTTGTTCCAATGGGAT
TTGCTCAGAAAGATGGTTCTTATGCAGGATTTGATATTGATTTAGCTACAGCTGTTTTTGAAAAATACGGAATCACGGTA
AATTGGCAACCGATTGATTGGGATTTGAAAGAAGCTGAATTGACAAAAGGAACGATTGATCTGATTTGGAATGGCTATTC
CGCTACAGACGAACGCCGTGAAAAGGTGGCTTTCAGTAACTCATATATGAAGAATGAGCAGGTATTGGTTACGAAGAAAT
CATCTGGTATCACGACTGCAAAGGATATGACTGGAAAGACATTAGGAGCTCAAGCTGGTTCATCTGGTTATGCGGACTTT
GAAGCAAATCCAGAACTTTTGAAGAATATTGTCGCTAATAAGGAAGCGAATCAATACCAAACCTTTAATGAAGCCTTGAT
TGATTTGAAAAACGATCGAATTGATGGTCTATTGATTGACCGTGTCTATGCAAACTATTATTTAGAAGCAGAAGGTGTTT
TAAACGATTATAATGTCTTTACAGTTGGACTAGAAACAGAAGCTTTTGCGGTTGGAGCCCGTAAGGAAGATACAAACTTG
GTTAAGAAGATAAATGAAGCTTTTTCTAGTCTTTACAAGGACGGCAAGTTCCAAGAAATCAGCCAAAAATGGTTTGGAGA
AGATGTAGCAACCAAAGAAGTAAAAGAAGGACAGTAA

Upstream 100 bases:

>100_bases
ACTTGGGATGACCCAGATTGTGGTTACCCATGATTTGCAGTTTGCTGAAAATATCGCAGATGTATTATTGAAAGTAGAAC
CTAAATAGGAGGAAAAATGG

Downstream 100 bases:

>100_bases
GATAAAATAGTGGCTGAAACTGCGTTTTGATTAGCAAAACGTAGTTTTTTTGTAATCTAGGAAAACGATAATAGCGATTG
AATCTGGATAATTGAATATG

Product: amino acid ABC transporter,extracellular amino acid-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIGFDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITV
NWQPIDWDLKEAELTKGTIDLIWNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYNVFTVGLETEAFAVGARKEDTNL
VKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKEGQ

Sequences:

>Translated_278_residues
MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIGFDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITV
NWQPIDWDLKEAELTKGTIDLIWNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYNVFTVGLETEAFAVGARKEDTNL
VKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKEGQ
>Mature_278_residues
MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIGFDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITV
NWQPIDWDLKEAELTKGTIDLIWNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYNVFTVGLETEAFAVGARKEDTNL
VKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKEGQ

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=244, Percent_Identity=31.9672131147541, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787031, Length=235, Percent_Identity=27.2340425531915, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787085, Length=234, Percent_Identity=27.7777777777778, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788649, Length=272, Percent_Identity=27.2058823529412, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788648, Length=239, Percent_Identity=26.3598326359833, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787088, Length=236, Percent_Identity=25, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 31020; Mature: 31020

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIGFDSTFVPMGFAQKDGS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEECCCEECCCEECCCCC
YAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLIWNGYSATDERREKVAFSN
CCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHC
SYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADFEANPELLKNIVANKEANQYQ
CCCCCCEEEEEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHH
TFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYNVFTVGLETEAFAVGARKEDTNL
HHHHHHHHCCCCCCCEEEEEEHHHHEEEEECCCCCCCEEEEEEECCCEEEECCCCCHHHH
VKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKEGQ
HHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCCC
>Mature Secondary Structure
MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIGFDSTFVPMGFAQKDGS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEECCCEECCCEECCCCC
YAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLIWNGYSATDERREKVAFSN
CCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHC
SYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADFEANPELLKNIVANKEANQYQ
CCCCCCEEEEEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHH
TFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYNVFTVGLETEAFAVGARKEDTNL
HHHHHHHHCCCCCCCEEEEEEHHHHEEEEECCCCCCCEEEEEEECCCEEEECCCCCHHHH
VKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKEGQ
HHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]