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The map label for this gene is gla [H]

Identifier: 221232209

GI number: 221232209

Start: 1411751

End: 1412620

Strand: Reverse

Name: gla [H]

Synonym: SPN23F_14560

Alternate gene names: 221232209

Gene position: 1412620-1411751 (Counterclockwise)

Preceding gene: 221232210

Following gene: 221232208

Centisome position: 63.59

GC content: 43.91

Gene sequence:

>870_bases
ATGGATTTCACATGGGCACTAAAGTATGCCACTGAATTTTTGGGAACTGCCATTTTGATCATTCTTGGGAATGGTGCAGT
TGCCAACGTTGAACTTAAAGGTACGAAAGGTCACCAAAGTGGCTGGATCGTCATCGCTGTTGGTTATGGTATGGGAGTTA
TGATCCCAGCCTTGATGTTTGGTAACGTATCTGGGAACCACATCAACCCTGCTTTCACTCTAGGACTTGCAGTTAGCGGT
CTTTTCCCTTGGGCACAAGTGGTACCTTACATTATCGCACAAGTCTTGGGGGCTATCTTTGGTCAAGCCTTAGTTGTGGC
AACACACCGTCCATTCTACTTGAAAACTGAAAACTCAAATAACATCTTGGGAACTTTCTCAACTATTTCAAGTATTGACC
ATGGTACAAAAGAAAGTCGCTATGCAGCAACTGTCAATGGTTTGATTAACGAGTTTGTTGGTTCATTTGTTTTGTTCTTT
GCAGCTCTTGGTTTGACTAAAAACTTCTTTGGTGCTGAAGTGCTTCAATACATGAAACAAATGGCAACACAAGCAGGACA
AACAGTTGATTTTTCTGACTTGGCTATTAAAGCACAGGTGGCTCCACACACTGCTTCAGGACTTTCTGTGGCTCACTTGG
CACTTGGATTCCTCGTTATGGCTTTGGTAACATCACTTGGAGGACCTACAGGACCTGCCTTGAACCCAGCCCGTGACTTG
GGACCACGTCTCCTTCATGCTTTCCTTCCAAAATCAGTTCTTGGTGAGCATAAAGGCGATTCAAAATGGTGGTATTCTTG
GGTACCAGTAGTAGCACCGATTGCAGCAGCAATTGCGGCAGTAGCTGTATTCAAATTCCTTTATCTCTAA

Upstream 100 bases:

>100_bases
ATCCTATTTTTTAAAAAATCTTTCCAGAAACTAGTTGCTAAATAGGGTAAAAATTGATAAAATAAGGAATATCTAAAAAA
TTTTAAGGAGAATCTAGCAA

Downstream 100 bases:

>100_bases
GAAATAGCTCCTTTAACATTTGAGTGAGCACCATCTATAAGTAAGAGAGGATCAGATTGGTTCTCTCTTTTTGATTTTCT
AGGAAATGAAAGAAAGTCTA

Product: glycerol facilitator-aquaporin

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin; Glyceroaquaporin [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIVIAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSG
LFPWAQVVPYIIAQVLGAIFGQALVVATHRPFYLKTENSNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
AALGLTKNFFGAEVLQYMKQMATQAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFLVMALVTSLGGPTGPALNPARDL
GPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAAAIAAVAVFKFLYL

Sequences:

>Translated_289_residues
MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIVIAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSG
LFPWAQVVPYIIAQVLGAIFGQALVVATHRPFYLKTENSNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
AALGLTKNFFGAEVLQYMKQMATQAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFLVMALVTSLGGPTGPALNPARDL
GPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAAAIAAVAVFKFLYL
>Mature_289_residues
MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIVIAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSG
LFPWAQVVPYIIAQVLGAIFGQALVVATHRPFYLKTENSNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
AALGLTKNFFGAEVLQYMKQMATQAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFLVMALVTSLGGPTGPALNPARDL
GPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAAAIAAVAVFKFLYL

Specific function: Mixed channel protein that transports both water and glycerol [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=287, Percent_Identity=28.2229965156794, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI157266307, Length=268, Percent_Identity=29.1044776119403, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI22538420, Length=286, Percent_Identity=30.0699300699301, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1790362, Length=276, Percent_Identity=30.0724637681159, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17558372, Length=289, Percent_Identity=25.9515570934256, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321054, Length=238, Percent_Identity=30.672268907563, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 30654; Mature: 30654

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIVIAVGYGMGVMIPALMF
CCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHH
GNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQALVVATHRPFYLKTENSN
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCC
NILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFFAALGLTKNFFGAEVLQYMKQ
CEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MATQAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFLVMALVTSLGGPTGPALNPARDL
HHHHCCCCCCHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
GPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAAAIAAVAVFKFLYL
HHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIVIAVGYGMGVMIPALMF
CCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHH
GNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQALVVATHRPFYLKTENSN
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCC
NILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFFAALGLTKNFFGAEVLQYMKQ
CEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MATQAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFLVMALVTSLGGPTGPALNPARDL
HHHHCCCCCCHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
GPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAAAIAAVAVFKFLYL
HHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]