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The map label for this gene is ogt [H]

Identifier: 221232188

GI number: 221232188

Start: 1387027

End: 1387539

Strand: Reverse

Name: ogt [H]

Synonym: SPN23F_14270

Alternate gene names: 221232188

Gene position: 1387539-1387027 (Counterclockwise)

Preceding gene: 221232189

Following gene: 221232187

Centisome position: 62.46

GC content: 42.5

Gene sequence:

>513_bases
ATGCAAAAGAAGTATGTAAAAATACTCTACTCCTCACCAATTGGTATTCTATCACTTGTAGCTGATGACCATTATTTGTA
TGGAATTTGGGTTCAGGAGCAGAAGCATTTTGAGAGGGGACTAGGAGATGAAACGATAGAAGAAGTTGTTAGTCATCCTA
TTTTAGACCCAGTTATTGCTTGCTTAGATGATTACTTTAAAGGCAAGCCTCAGGATTTATCCAACTTGCTCTTGGCGCCA
ATCGGAACGAATTTTGAAAAGAGAGTTTGGGACTATTTACAGGGCATTCCTTATGGTCAGACAGTGACCTATGGACAAAT
TGCTCAAGACCTGCAAGTGGCTTCTGCTCAAGCAATTGGTGGAGCAGTGGGACGCAATCCTTGGTCTATCCTAGTACCTT
GTCATCGTGTGTTGGGAGCAGGCAAGCGTCTGACAGGCTATGCTGCAGGAGTGGAAAAGAAAGCTTGGCTCTTGGAGCAT
GAAGGCGTAGATTTTAAAGATATAAACAAATAG

Upstream 100 bases:

>100_bases
ACAAGGTTATGCAACATATTTTTGAAAAACTTGGTTTTAAACAAGTTGGTAAGGTGCCAGTAGATGGCGAACGCTTGGCC
TATCAAAAATTAAAGAAATA

Downstream 100 bases:

>100_bases
AAGGAGAAGCACATGTTAGAATTTATCGAATACCCCAAATGTTCAACTTGTAAAAAAGCAAAACAAGAATTAAATCAATT
AGGTGTGGACTATAAAGCCG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDETIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAP
IGTNFEKRVWDYLQGIPYGQTVTYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
EGVDFKDINK

Sequences:

>Translated_170_residues
MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDETIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAP
IGTNFEKRVWDYLQGIPYGQTVTYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
EGVDFKDINK
>Mature_170_residues
MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDETIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAP
IGTNFEKRVWDYLQGIPYGQTVTYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
EGVDFKDINK

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=49.0196078431373, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788542, Length=82, Percent_Identity=40.2439024390244, Blast_Score=69, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115533068, Length=118, Percent_Identity=34.7457627118644, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=40, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6320001, Length=82, Percent_Identity=40.2439024390244, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18939; Mature: 18939

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDETIEEVVSHPILDPVIA
CCCCEEEEEECCCCHHHHHEECCCEEEEEEECCHHHHHHCCCHHHHHHHHHCCCHHHHHH
CLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTVTYGQIAQDLQVASAQAIG
HHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
GAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEHEGVDFKDINK
HHCCCCCCEEHEEHHHHHCCCCCHHHHHCCCCHHHEEEECCCCCHHCCCC
>Mature Secondary Structure
MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDETIEEVVSHPILDPVIA
CCCCEEEEEECCCCHHHHHEECCCEEEEEEECCHHHHHHCCCHHHHHHHHHCCCHHHHHH
CLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTVTYGQIAQDLQVASAQAIG
HHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
GAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEHEGVDFKDINK
HHCCCCCCEEHEEHHHHHCCCCCHHHHHCCCCHHHEEEECCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]