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The map label for this gene is mtbP [H]

Identifier: 221232093

GI number: 221232093

Start: 1286193

End: 1287551

Strand: Reverse

Name: mtbP [H]

Synonym: SPN23F_13150

Alternate gene names: 221232093

Gene position: 1287551-1286193 (Counterclockwise)

Preceding gene: 221232094

Following gene: 221232092

Centisome position: 57.96

GC content: 38.78

Gene sequence:

>1359_bases
ATGAGATTTATTGATTTATTTTCAGGTATCGGTGGTTTTCGACTTGGAATGGAAAGTGTAGGACACGAGTGTATTGGATT
TTGTGAGATTGATAAATTTGCTAGAGAATCTTATAAGTCCATTTTTCAAACGGAAGGAGAAATAGAATTTCATGACATAC
GAGATGTTTCAGATGACGAATTTAAAAAACTTAGAGGGAAAGTCGATATCATCTGTGGGGGATTTCCTTGTCAAGCATTT
TCAATCGCAGGAAGACGATTGGGATTTGAAGATATTAGAGGAACTTTGTTCTTTGAAATTGCTCGGGCGGCCAAACAAAT
CCAACCACGTTTTCTTTTTCTTGAAAATGTTAAAGGTCTACTCAATCACGATAAGGGACGGACGTTCACCACAATCCTTA
CCACACTTGATGAGTTGGGGTTTGATGTTGAATGGCAGGTGCTTAACAGTAAGGATTTTGGCGTTCCCCAAAACAGAGAG
AGGGTGTTTATTATCGGACATTCTAGAAAGAAAGGTATCAGACTCTTATTTCCTTTCAGACGAGAAGGTCAAGCAACTAA
CTCTGAGACTTTAAAAACATTAGGAAATTTGAATCCATCAAAAAGTGGAATGAGCGGTAAAGTTTATTATTCAGAAGGTC
TTGCGCCAACCTTAGTTCGTGGGAAAGGAGAAGGATTTAAGATTGCGATTCCTTGTATGACACCAGATAGATTAGACAAG
AGACAAAATGGTAGACGCTTTAAGGAAAACCAAGAGCCAATGTTTACTTTAAATACTCAGGATCGTCATGGCATTGTCGT
TGTTGGAGATTTACCGACTAGCTTTAAGGAGACCGGTCGTGTCTATGGAAGTGAGGGGTTATCTCCAACACTGACTACGA
TGCAAGGTGGAGATAAAATCCCCAAAATACTGATTCCAGAACCAATTCAATTTCTAAAAGTCCGGGAAGCAACGAAAAAA
GGATACGCTCAAGCAGAAATAGGAGATTCAATCAATTTGGAAAGACCAAGTTCTCAGTATCGTCGTGGTAGAGTTGGAAA
AGGTATAGCTAATACGTTAACAACTAGTGGACAAATGGGAGTAGTAGTTGCTAGTTATGAAGGAGAAGATAAACAAGTTT
ATCATGTAGCCGGTATATTAATAGATGGACAATTTTACCGTCTGAGGATACGACGAATCACTCCTAAAGAGTGTTTTCGC
TTGCAGGGCTTTCCTGATTGGGCTTTTGAAGCTGCTAGAAGAGTCTCTAGTAATAGTCAGCTCTATAAACAAGCTGGTAA
TAGTGTAACCGTTCCTGTGATTGCCGCAATCGCAAAGAAATTAAAAGAAATAGAGGAAAAAGATGAAAGCATTAAATAA

Upstream 100 bases:

>100_bases
AAATGTCGCCAATCGAGTGGATATTAGTTATGCTCATTGTCATTTCAAGTGGTGTGACACTTTGGACATTGATAGTTGAT
CAGAAAGGGGTAATCAAGAA

Downstream 100 bases:

>100_bases
AGAATCAATACTAGATTGTGATGAATTAGAAACAGAATTACATGATGCAGAAATCAAACAGCTGGATGAACAATTATTTT
TGATACCCAATTATCCATGT

Product: DNA methylase

Products: NA

Alternate protein names: M.Phi3TI; Cytosine-specific methyltransferase Phi3TI [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQTEGEIEFHDIRDVSDDEFKKLRGKVDIICGGFPCQAF
SIAGRRLGFEDIRGTLFFEIARAAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRE
RVFIIGHSRKKGIRLLFPFRREGQATNSETLKTLGNLNPSKSGMSGKVYYSEGLAPTLVRGKGEGFKIAIPCMTPDRLDK
RQNGRRFKENQEPMFTLNTQDRHGIVVVGDLPTSFKETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKK
GYAQAEIGDSINLERPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGILIDGQFYRLRIRRITPKECFR
LQGFPDWAFEAARRVSSNSQLYKQAGNSVTVPVIAAIAKKLKEIEEKDESIK

Sequences:

>Translated_452_residues
MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQTEGEIEFHDIRDVSDDEFKKLRGKVDIICGGFPCQAF
SIAGRRLGFEDIRGTLFFEIARAAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRE
RVFIIGHSRKKGIRLLFPFRREGQATNSETLKTLGNLNPSKSGMSGKVYYSEGLAPTLVRGKGEGFKIAIPCMTPDRLDK
RQNGRRFKENQEPMFTLNTQDRHGIVVVGDLPTSFKETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKK
GYAQAEIGDSINLERPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGILIDGQFYRLRIRRITPKECFR
LQGFPDWAFEAARRVSSNSQLYKQAGNSVTVPVIAAIAKKLKEIEEKDESIK
>Mature_452_residues
MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQTEGEIEFHDIRDVSDDEFKKLRGKVDIICGGFPCQAF
SIAGRRLGFEDIRGTLFFEIARAAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRE
RVFIIGHSRKKGIRLLFPFRREGQATNSETLKTLGNLNPSKSGMSGKVYYSEGLAPTLVRGKGEGFKIAIPCMTPDRLDK
RQNGRRFKENQEPMFTLNTQDRHGIVVVGDLPTSFKETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKK
GYAQAEIGDSINLERPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGILIDGQFYRLRIRRITPKECFR
LQGFPDWAFEAARRVSSNSQLYKQAGNSVTVPVIAAIAKKLKEIEEKDESIK

Specific function: This enzyme methylates the first cytosine within the sequences GGCC and GCNGC [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4758184, Length=208, Percent_Identity=25.4807692307692, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788271, Length=291, Percent_Identity=32.6460481099656, Blast_Score=132, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 50804; Mature: 50804

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQTEGEIEFHDIRDVSDDE
CCHHHHHCCCCHHHHCHHHCCHHHEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
FKKLRGKVDIICGGFPCQAFSIAGRRLGFEDIRGTLFFEIARAAKQIQPRFLFLENVKGL
HHHHCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
LNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRERVFIIGHSRKKGIRLLFPFR
HCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECCC
REGQATNSETLKTLGNLNPSKSGMSGKVYYSEGLAPTLVRGKGEGFKIAIPCMTPDRLDK
CCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEECCCCCEEEEEECCCCHHHHH
RQNGRRFKENQEPMFTLNTQDRHGIVVVGDLPTSFKETGRVYGSEGLSPTLTTMQGGDKI
HHCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCHHHHCCEECCCCCCCEEEECCCCCCC
PKILIPEPIQFLKVREATKKGYAQAEIGDSINLERPSSQYRRGRVGKGIANTLTTSGQMG
CEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCE
VVVASYEGEDKQVYHVAGILIDGQFYRLRIRRITPKECFRLQGFPDWAFEAARRVSSNSQ
EEEEECCCCCCEEEEEEEEEECCEEEEEEEEECCHHHHHCCCCCCCHHHHHHHHHCCCHH
LYKQAGNSVTVPVIAAIAKKLKEIEEKDESIK
HHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQTEGEIEFHDIRDVSDDE
CCHHHHHCCCCHHHHCHHHCCHHHEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
FKKLRGKVDIICGGFPCQAFSIAGRRLGFEDIRGTLFFEIARAAKQIQPRFLFLENVKGL
HHHHCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
LNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRERVFIIGHSRKKGIRLLFPFR
HCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECCC
REGQATNSETLKTLGNLNPSKSGMSGKVYYSEGLAPTLVRGKGEGFKIAIPCMTPDRLDK
CCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEECCCCCEEEEEECCCCHHHHH
RQNGRRFKENQEPMFTLNTQDRHGIVVVGDLPTSFKETGRVYGSEGLSPTLTTMQGGDKI
HHCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCHHHHCCEECCCCCCCEEEECCCCCCC
PKILIPEPIQFLKVREATKKGYAQAEIGDSINLERPSSQYRRGRVGKGIANTLTTSGQMG
CEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCE
VVVASYEGEDKQVYHVAGILIDGQFYRLRIRRITPKECFRLQGFPDWAFEAARRVSSNSQ
EEEEECCCCCCEEEEEEEEEECCEEEEEEEEECCHHHHHCCCCCCCHHHHHHHHHCCCHH
LYKQAGNSVTVPVIAAIAKKLKEIEEKDESIK
HHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]