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The map label for this gene is ltrBE1 [H]

Identifier: 221232025

GI number: 221232025

Start: 1209814

End: 1211643

Strand: Reverse

Name: ltrBE1 [H]

Synonym: SPN23F_12430

Alternate gene names: 221232025

Gene position: 1211643-1209814 (Counterclockwise)

Preceding gene: 221232026

Following gene: 221232024

Centisome position: 54.55

GC content: 28.31

Gene sequence:

>1830_bases
ATGGTCATCACTAAACACTTTGCGATTCATGGAAAAAATTATCGTAGCAAACTAGTCAAATATATTTTGAATCCAAGTAA
AACAAAAAATCTAGCACTAGTTTCAGATTTTGGTATGAGAAATTATTTAGATTTTCCTAGTTATAAAGAACTAGTGAAGA
TGTACAATGATAATTTTTTAAGTAATGATGGTCTTTATGAATTTCGTCATGATAGGCAAGAAGTAAATCAACGAAGAATT
TATTCTCATCATATTATTCAGTCTTTTTCTCCAGATGATCATCTTACTCCTGAACAAATCAATCGGATTGGTTATGAGAC
AGTTAAAGAGTTGACAGGAGGTAGATTTCGTTTTATCGTAGCAACACATGTCGATAAAGGTCATATCCACAATCACATCA
TCCTAAATTCAATTGATCAGAATTCTGATAAAAAGTTTCTATGGAATTATAAGTCAGAACGTAATCTACGAATGGTTTCA
GATCGTCTCTCAAAAATTGCAGGTGCAAAGATTATAGAAAATCGTTATTCGCATCGTCAGTATGAAGTTTATCGAAAAAC
CAATTACAAATATGAAATAAAACAACGGGTATATTTTCTAATTGAGAACTCGAAAAATTTTGAAGATTTCAGGAAAAAAG
CAAAAGCTTTACATTTAATAATTGATTTTAGACACAAGCATGTTACTTTTTTTATGACTGATTCAAATATGAAACAAGTC
GTACGTGATGATAAATTGAATAGAAAACAACCTTATAATGAAACTTATTTTAAGCAAAAGTTTGTTCAAAGGGAAATTAT
AAACATCTTAGAATTTCTACTTCCGAAAATGAAGAATATGAATGAATTGATTCAACAAGCAGAATTTTTTGACTTAAAAA
TAATTCCGAAAGAAAAACATGTTCTATTTGAATTTAATGGGATTAAGCTTTCAGAACAGGAATTGGGAAAAATGAATCAG
TACAGTGTTAGTTATTTTCAAGACTATTTTAATAACAAAAATGAAACTTTTGTCTTAGATAATAATAATTTAATTGAACT
TTACAATAAAGAAAAGCTAATTAAAGAAAAAGAGTTGCCGACAGAAGAGGTGGTATGGAAATCCTATCAAGATTTCAAGA
GAAATAGGGATGCTGTTCATGAGTTAGAAGTAGAGTTGAATCTTAATCAAATAGAAGCAGTAGTAGACGATGGAATTTAC
ATTAAGGTACAGTTTGGTATCCGACAAGAAGGACTTATTTTTGTACCAAATATTCAAATCAATATGGAAGAAGAAAAAGT
TAAAGTATTTCTCAGAGAAACTAGTTCTTACTATGTATATCATAAAGATTCAGCAGACAAAAATCGCTTTATGAAAGGTA
AAACTTTAATTAGGCAATTTACGCTTCAGCATGAACCGCAGCATATGTATAGAAGAATACCTCTTAGCAAAATTAAAGAA
AAAATAGAACAATTAGATTTTCTTATATCTGCGGAAAATAGTCCGAATGATTTTGAAGATATAACAAATGATTTCATTGC
CCAAATATCATATCTAGAGAATATGATTGAACAAGTCCAAAATAAAATTGATGATTTAACTAATTTAGAGGAAGTATTGT
TGAATAATACGACAAATAGTTCTAGCAATTTAGAAAATAGTATTCAAGGTAAAAGTTCAGTAGATACGATAGAGAAGGAT
TTATACATATATAAAGGAAAGATTGAAACACTGAAGGAACAACATGGAGAAGCAATAAATTTATTTGAAATGTTTAATAA
AACAATAAAGAAATATAAGAAAAAACAAAATATGAAATCTATTGAGGAAAATGAGATACATTTAGAGTAA

Upstream 100 bases:

>100_bases
ACGGAGGTTGAATTAAATGAGTTGAGAAAAGGTATAGAAGAGTTAATAGTAGAAGTGGAGAAAGATTTTCTTATTCAATC
TGAAAAATTGAGGAAATTTT

Downstream 100 bases:

>100_bases
ACAGTTTCCTAAAAAGTATATATAGAATAGTTTCATGTGCCAATTTGTCACATATTTAAAAATAAGAAAATAACTATATT
ATACTTGTTTTAGGAGACAA

Product: relaxase

Products: NA

Alternate protein names: Conjugative nickase [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MVITKHFAIHGKNYRSKLVKYILNPSKTKNLALVSDFGMRNYLDFPSYKELVKMYNDNFLSNDGLYEFRHDRQEVNQRRI
YSHHIIQSFSPDDHLTPEQINRIGYETVKELTGGRFRFIVATHVDKGHIHNHIILNSIDQNSDKKFLWNYKSERNLRMVS
DRLSKIAGAKIIENRYSHRQYEVYRKTNYKYEIKQRVYFLIENSKNFEDFRKKAKALHLIIDFRHKHVTFFMTDSNMKQV
VRDDKLNRKQPYNETYFKQKFVQREIINILEFLLPKMKNMNELIQQAEFFDLKIIPKEKHVLFEFNGIKLSEQELGKMNQ
YSVSYFQDYFNNKNETFVLDNNNLIELYNKEKLIKEKELPTEEVVWKSYQDFKRNRDAVHELEVELNLNQIEAVVDDGIY
IKVQFGIRQEGLIFVPNIQINMEEEKVKVFLRETSSYYVYHKDSADKNRFMKGKTLIRQFTLQHEPQHMYRRIPLSKIKE
KIEQLDFLISAENSPNDFEDITNDFIAQISYLENMIEQVQNKIDDLTNLEEVLLNNTTNSSSNLENSIQGKSSVDTIEKD
LYIYKGKIETLKEQHGEAINLFEMFNKTIKKYKKKQNMKSIEENEIHLE

Sequences:

>Translated_609_residues
MVITKHFAIHGKNYRSKLVKYILNPSKTKNLALVSDFGMRNYLDFPSYKELVKMYNDNFLSNDGLYEFRHDRQEVNQRRI
YSHHIIQSFSPDDHLTPEQINRIGYETVKELTGGRFRFIVATHVDKGHIHNHIILNSIDQNSDKKFLWNYKSERNLRMVS
DRLSKIAGAKIIENRYSHRQYEVYRKTNYKYEIKQRVYFLIENSKNFEDFRKKAKALHLIIDFRHKHVTFFMTDSNMKQV
VRDDKLNRKQPYNETYFKQKFVQREIINILEFLLPKMKNMNELIQQAEFFDLKIIPKEKHVLFEFNGIKLSEQELGKMNQ
YSVSYFQDYFNNKNETFVLDNNNLIELYNKEKLIKEKELPTEEVVWKSYQDFKRNRDAVHELEVELNLNQIEAVVDDGIY
IKVQFGIRQEGLIFVPNIQINMEEEKVKVFLRETSSYYVYHKDSADKNRFMKGKTLIRQFTLQHEPQHMYRRIPLSKIKE
KIEQLDFLISAENSPNDFEDITNDFIAQISYLENMIEQVQNKIDDLTNLEEVLLNNTTNSSSNLENSIQGKSSVDTIEKD
LYIYKGKIETLKEQHGEAINLFEMFNKTIKKYKKKQNMKSIEENEIHLE
>Mature_609_residues
MVITKHFAIHGKNYRSKLVKYILNPSKTKNLALVSDFGMRNYLDFPSYKELVKMYNDNFLSNDGLYEFRHDRQEVNQRRI
YSHHIIQSFSPDDHLTPEQINRIGYETVKELTGGRFRFIVATHVDKGHIHNHIILNSIDQNSDKKFLWNYKSERNLRMVS
DRLSKIAGAKIIENRYSHRQYEVYRKTNYKYEIKQRVYFLIENSKNFEDFRKKAKALHLIIDFRHKHVTFFMTDSNMKQV
VRDDKLNRKQPYNETYFKQKFVQREIINILEFLLPKMKNMNELIQQAEFFDLKIIPKEKHVLFEFNGIKLSEQELGKMNQ
YSVSYFQDYFNNKNETFVLDNNNLIELYNKEKLIKEKELPTEEVVWKSYQDFKRNRDAVHELEVELNLNQIEAVVDDGIY
IKVQFGIRQEGLIFVPNIQINMEEEKVKVFLRETSSYYVYHKDSADKNRFMKGKTLIRQFTLQHEPQHMYRRIPLSKIKE
KIEQLDFLISAENSPNDFEDITNDFIAQISYLENMIEQVQNKIDDLTNLEEVLLNNTTNSSSNLENSIQGKSSVDTIEKD
LYIYKGKIETLKEQHGEAINLFEMFNKTIKKYKKKQNMKSIEENEIHLE

Specific function: Mediates initition of conjugal transfer of plasmid pRS01 possibly by introducing a single-stranded nick at the potential origin of transfer, to initiate single strand transfer from donor to recipient [H]

COG id: COG3843

COG function: function code U; Type IV secretory pathway, VirD2 components (relaxase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mobilization (MOB) protein type 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005094 [H]

Pfam domain/function: PF03432 Relaxase [H]

EC number: NA

Molecular weight: Translated: 72988; Mature: 72988

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVITKHFAIHGKNYRSKLVKYILNPSKTKNLALVSDFGMRNYLDFPSYKELVKMYNDNFL
CEEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCC
SNDGLYEFRHDRQEVNQRRIYSHHIIQSFSPDDHLTPEQINRIGYETVKELTGGRFRFIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
ATHVDKGHIHNHIILNSIDQNSDKKFLWNYKSERNLRMVSDRLSKIAGAKIIENRYSHRQ
EEECCCCCCCCEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
YEVYRKTNYKYEIKQRVYFLIENSKNFEDFRKKAKALHLIIDFRHKHVTFFMTDSNMKQV
EEEHHHCCCEEEEEEEEEEEEECCCCHHHHHHHHHHEEEEEEECCCEEEEEEECCHHHHH
VRDDKLNRKQPYNETYFKQKFVQREIINILEFLLPKMKNMNELIQQAEFFDLKIIPKEKH
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEECCCCE
VLFEFNGIKLSEQELGKMNQYSVSYFQDYFNNKNETFVLDNNNLIELYNKEKLIKEKELP
EEEEECCCEECHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEECCHHHHHHHHCCC
TEEVVWKSYQDFKRNRDAVHELEVELNLNQIEAVVDDGIYIKVQFGIRQEGLIFVPNIQI
HHHHHHHHHHHHHHCCCCHHHEEEEECHHHHHHHHCCCEEEEEEECCCCCCEEEECCEEE
NMEEEKVKVFLRETSSYYVYHKDSADKNRFMKGKTLIRQFTLQHEPQHMYRRIPLSKIKE
EECHHHHHHHEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHH
KIEQLDFLISAENSPNDFEDITNDFIAQISYLENMIEQVQNKIDDLTNLEEVLLNNTTNS
HHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SSNLENSIQGKSSVDTIEKDLYIYKGKIETLKEQHGEAINLFEMFNKTIKKYKKKQNMKS
CCCCHHHCCCCCHHHHHHHHHEEEECHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHC
IEENEIHLE
CCCCCCCCC
>Mature Secondary Structure
MVITKHFAIHGKNYRSKLVKYILNPSKTKNLALVSDFGMRNYLDFPSYKELVKMYNDNFL
CEEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCC
SNDGLYEFRHDRQEVNQRRIYSHHIIQSFSPDDHLTPEQINRIGYETVKELTGGRFRFIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
ATHVDKGHIHNHIILNSIDQNSDKKFLWNYKSERNLRMVSDRLSKIAGAKIIENRYSHRQ
EEECCCCCCCCEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
YEVYRKTNYKYEIKQRVYFLIENSKNFEDFRKKAKALHLIIDFRHKHVTFFMTDSNMKQV
EEEHHHCCCEEEEEEEEEEEEECCCCHHHHHHHHHHEEEEEEECCCEEEEEEECCHHHHH
VRDDKLNRKQPYNETYFKQKFVQREIINILEFLLPKMKNMNELIQQAEFFDLKIIPKEKH
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEECCCCE
VLFEFNGIKLSEQELGKMNQYSVSYFQDYFNNKNETFVLDNNNLIELYNKEKLIKEKELP
EEEEECCCEECHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEECCHHHHHHHHCCC
TEEVVWKSYQDFKRNRDAVHELEVELNLNQIEAVVDDGIYIKVQFGIRQEGLIFVPNIQI
HHHHHHHHHHHHHHCCCCHHHEEEEECHHHHHHHHCCCEEEEEEECCCCCCEEEECCEEE
NMEEEKVKVFLRETSSYYVYHKDSADKNRFMKGKTLIRQFTLQHEPQHMYRRIPLSKIKE
EECHHHHHHHEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHH
KIEQLDFLISAENSPNDFEDITNDFIAQISYLENMIEQVQNKIDDLTNLEEVLLNNTTNS
HHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SSNLENSIQGKSSVDTIEKDLYIYKGKIETLKEQHGEAINLFEMFNKTIKKYKKKQNMKS
CCCCHHHCCCCCHHHHHHHHHEEEECHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHC
IEENEIHLE
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8655550; 10532373 [H]