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The map label for this gene is ispD [H]

Identifier: 221231959

GI number: 221231959

Start: 1139102

End: 1139809

Strand: Reverse

Name: ispD [H]

Synonym: SPN23F_11650

Alternate gene names: 221231959

Gene position: 1139809-1139102 (Counterclockwise)

Preceding gene: 221231963

Following gene: 221231958

Centisome position: 51.31

GC content: 38.7

Gene sequence:

>708_bases
ATGATTTATGCAGGAATTCTTGCCGGTGGAACTGGCACACGCATGGGGATCAGTAACTTGCCAAAACAATTTTTAGAGCT
AGGTAATCGACCTATTTTGATTCATACAATTGAAAAATTTGTCTTGGAGCCAAGTATTGAAAAAATTGTAGTTGGTGTTC
ATGGAGACTGGGTTTCTCATGCAGAAGATCTTGTAGATAAATATCTTCCTCTTTATAAGGAACGTATCATCATTACAAAG
GGTGGTGCTGACCGCAATACAAGTATTAAGAAAATCATTGAAGCCATTGATGCTTATCGTCCGCTTACTCCAGAGGATAT
CGTTGTTACCCACGATTCTGTTCGTCCATTTATTACACTTCGCATGATTCAGGACAATATCCAACTTGCCCAAAATCATG
ACGCAGTGGACACAGTGGTAGAAGCGGTTGATACTATCGTTGAAAGTACCAATGGTCAATTTATTACAGATATTCCAAAT
CGTGCTCACCTTTATCAAGGACAAACACCTCAAACATTCCGTTGCAAGGACTTCATGGACCTTTATGGATCTCTTTCTGA
TGAAGAGAAGGAAATCTTGACAGATGCATGTAAAATCTTTGTGATCAAAGGAAAAGATGTGGCCTTGGCCAAAGGTGAAT
ACTCAAATCTGAAGATTACAACCGTAACAGATTTGAAGATTGCAAAAAGTATGATTGAGAAAGACTAG

Upstream 100 bases:

>100_bases
CTATTCTAGAAGGGGAGGACCAGTATTTCTTTTATGATAGGACTAGATTGTGGTATAATAGAGAGAATAAGTTTTTTTAG
TAAGACAAAGGAGAAAATAG

Downstream 100 bases:

>100_bases
TAAAATGATTAATCAAATTTATCAACTAACTAAGCCTAAGTTTATCAATGTCAAATATCAGGAAGAGGCTATTGACCAAG
AGAATCATATCCTTATCCGT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MIYAGILAGGTGTRMGISNLPKQFLELGNRPILIHTIEKFVLEPSIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITK
GGADRNTSIKKIIEAIDAYRPLTPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLKITTVTDLKIAKSMIEKD

Sequences:

>Translated_235_residues
MIYAGILAGGTGTRMGISNLPKQFLELGNRPILIHTIEKFVLEPSIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITK
GGADRNTSIKKIIEAIDAYRPLTPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLKITTVTDLKIAKSMIEKD
>Mature_235_residues
MIYAGILAGGTGTRMGISNLPKQFLELGNRPILIHTIEKFVLEPSIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITK
GGADRNTSIKKIIEAIDAYRPLTPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLKITTVTDLKIAKSMIEKD

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=240, Percent_Identity=27.9166666666667, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI157671913, Length=202, Percent_Identity=26.2376237623762, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 26256; Mature: 26256

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYAGILAGGTGTRMGISNLPKQFLELGNRPILIHTIEKFVLEPSIEKIVVGVHGDWVSH
CEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHCCCHHEEEEEECCCHHHH
AEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPLTPEDIVVTHDSVRPFITL
HHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEE
RMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPNRAHLYQGQTPQTFRCKDFMD
EEHHCCHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCCHHHHHH
LYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLKITTVTDLKIAKSMIEKD
HHCCCCCHHHHHHHHHHEEEEECCCCEEEECCCCCCEEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MIYAGILAGGTGTRMGISNLPKQFLELGNRPILIHTIEKFVLEPSIEKIVVGVHGDWVSH
CEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHCCCHHEEEEEECCCHHHH
AEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPLTPEDIVVTHDSVRPFITL
HHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEE
RMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPNRAHLYQGQTPQTFRCKDFMD
EEHHCCHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCCHHHHHH
LYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLKITTVTDLKIAKSMIEKD
HHCCCCCHHHHHHHHHHEEEEECCCCEEEECCCCCCEEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA