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The map label for this gene is ybjI [H]

Identifier: 221231940

GI number: 221231940

Start: 1115167

End: 1115961

Strand: Reverse

Name: ybjI [H]

Synonym: SPN23F_11410

Alternate gene names: 221231940

Gene position: 1115961-1115167 (Counterclockwise)

Preceding gene: 221231941

Following gene: 221231939

Centisome position: 50.24

GC content: 39.87

Gene sequence:

>795_bases
ATGACAATTAAACTAGTAGCAACGGATATGGACGGAACCTTCCTAGATGGGAATGGACGCTTTGATATGGATCGTCTCAA
GTCTCTCTTGGCTTCCTACAAGGAAAAAGGGATTTACTTTGCGGTGGCTTCGGGTCGGGGATTTCTGTCTCTGGAAAAAT
TATTTGCTGATGTTCGTGATGACATTATTTTCATCGCGGAAAATGGCAGTTTGGTAGAGTATCAAGGTCAGGACTTGTAT
GAAGCGACTATGTCTCGTGACTTTTATCTGGTAACTTTTGAAAAGCTGAAAACGTCACCTTATGTAGATATCAATAAACT
GCTCTTGACGGGTAATAAGGGTTCATATGTTCTAGATACGGTTGATGAGACCTATTTGAAAGTGAGTCAGCATTATAATG
AAAATATCCAAAAAGTAGCGAGTTTGGAAGATATCACAGATGACATTTTCAAATTTACAACCAACTTCACAGAAGAAACG
CTGGAAGATGGGGAGGCTTGGGTAAACGAAAACGTTCCTGGTGTTAAGGCTATGACAACTGGCTTTGAATCTATTGATAT
TGTTCTGGACTATGTCGATAAGGGAGTGGCCATTGTTGAATTAGTTAAAAAACTTGGTATCACAATGGATCAGGTCATGG
CTTTTGGAGACAATCTTAATGACTTACATATGATGCAGGTTGTGGGACATCCTGTAGCTCCTGAAAATGCACGACCTGAA
ATTTTAGAATTAGCAAAGACTGTGATTGGTCACCATAAGGACCAGTCGGTTATAGCTTATATGGAGGGCTTATAA

Upstream 100 bases:

>100_bases
CCATGGCAGCGGCTGATTCTATCTATGGAGTGACCATGCAAGAATCAGGTGTCTCAAAAATTGTTTCAGTTAAGTTAAAA
GATTTAGAAAGTATTGAAGG

Downstream 100 bases:

>100_bases
TGGCAGATATAAAATTAATCGCATTGGACTTGGACGGGACCTTGCTGACTACTGATAAAAGGCTGACGGATCGTACCAAG
GAAACCTTGAAAGCTGCGCG

Product: haloacid dehalogenase-like hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVASGRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLY
EATMSRDFYLVTFEKLKTSPYVDINKLLLTGNKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDNLNDLHMMQVVGHPVAPENARPE
ILELAKTVIGHHKDQSVIAYMEGL

Sequences:

>Translated_264_residues
MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVASGRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLY
EATMSRDFYLVTFEKLKTSPYVDINKLLLTGNKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDNLNDLHMMQVVGHPVAPENARPE
ILELAKTVIGHHKDQSVIAYMEGL
>Mature_263_residues
TIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVASGRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYE
ATMSRDFYLVTFEKLKTSPYVDINKLLLTGNKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEETL
EDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDNLNDLHMMQVVGHPVAPENARPEI
LELAKTVIGHHKDQSVIAYMEGL

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=259, Percent_Identity=31.2741312741313, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787043, Length=248, Percent_Identity=29.0322580645161, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI2367265, Length=266, Percent_Identity=27.8195488721804, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 29582; Mature: 29451

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVASGRGFLSLEKLFADVRD
CEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCC
DIIFIAENGSLVEYQGQDLYEATMSRDFYLVTFEKLKTSPYVDINKLLLTGNKGSYVLDT
CEEEEECCCCEEEECCCHHHHHHHCCCEEEEEHHHHCCCCCEEEEEEEEECCCCCEEEEC
VDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEETLEDGEAWVNENVPGVKAMTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHCCCCCCCHHHHC
GFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDNLNDLHMMQVVGHPVAPENARPE
CCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHH
ILELAKTVIGHHKDQSVIAYMEGL
HHHHHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVASGRGFLSLEKLFADVRD
EEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCC
DIIFIAENGSLVEYQGQDLYEATMSRDFYLVTFEKLKTSPYVDINKLLLTGNKGSYVLDT
CEEEEECCCCEEEECCCHHHHHHHCCCEEEEEHHHHCCCCCEEEEEEEEECCCCCEEEEC
VDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEETLEDGEAWVNENVPGVKAMTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHCCCCCCCHHHHC
GFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDNLNDLHMMQVVGHPVAPENARPE
CCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHH
ILELAKTVIGHHKDQSVIAYMEGL
HHHHHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]